FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-48-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-48-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574535
Total Bases397.3 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA323701719.53006223100678No Hit
GCACTGGTGGTTCAGTGGTAGAATT168466910.164200684966426No Hit
AACTTTTGACGGTGGATCTCTAGGC5798613.4985053879339603No Hit
GGAAGAGCACACGTCTGAACTCCAG4304872.5972795013555436Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTC4063682.4517610901301303No Hit
CTCGGGCTGAGACTTGAAGCG1451980.8760306096068456No Hit
CGACCAAGATAGTCGAACTCGACCC1304070.7867913036474325No Hit
CCCTTTCCGAGGTCGCGGTC1223760.7383374556209269No Hit
TCCCTTTCCGAGGTCGCGGTC1208340.729034027198953No Hit
CTTTCCGAGGTCGCGGTC1176220.7096549013290568No Hit
CTCCCTTTCCGAGGTCGCGGTC989050.5967286563393784No Hit
CTCGGGCTGAGACTTGAAGCA974450.587919962762153No Hit
GGATCCGGCCCGGACTGGCC893860.539297180886221No Hit
CTCGGGCTGAGACTTGAAGCGGTT838800.5060775460669032No Hit
GTTGGATCCGGCCCGGACTGGCC801240.4834162768367257No Hit
CAACTTTTGACGGTGGATCTCTAGG794220.4791808638975392No Hit
TCAAGTCTAGGCTGGTTAGTTT723670.43661556719389105No Hit
TGAAAATCTTTGCTCTGAAGTGGA722680.436018265369134No Hit
TCTCGGGCTGAGACTTGAAGCGGTT679540.40999038585396214No Hit
GCCGAGCTGTTCTTCCTCGCCGAGA609040.36745525590914013No Hit
TGAAAATCTTTGCTCTGAAGTGGAT586070.35359664690442294No Hit
ACCCGTAGATCCGAACTTGT584100.352408076606674No Hit
CACTACCGATAGCCAACTCGAACGT571010.34451041914599717No Hit
TTCCTGCACGGCTTCAGGCAATTCT562230.33921313629613137No Hit
GGCCGAGCTGTTCTTCCTCGCCGAG561650.3388632018937484No Hit
CCTTTCCGAGGTCGCGGTC560020.3378797655560171No Hit
GGATCCGGCCCGGACTGGC539710.32562602812084923No Hit
GCACTTGTGGTTCAGTGGTAGAATT526140.31743876977544166No Hit
TTCTGCTGAACTACTTTGAGAACGA523060.315580497431753No Hit
TCTCGGGCTGAGACTTGAAGCG505460.30496179832496056No Hit
ACCCGTAGATCCGAACTTGTGG449620.2712715620679555No Hit
GGATCCGGCCCGGACTGGCA434070.2618897000730337No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC411310.24815779145538625No Hit
GTTGGATCCGGCCCGGACTGGCA406290.2451290488692443No Hit
AGACTTGAAGCGGTTGGATCCGGCC400980.24192533908191088No Hit
TGAAAATCTTTGCTCTGAAGTGGAG373640.22543015535579128No Hit
ACACTGGTGGTTCAGTGGTAGAATT366410.22106804202953506No Hit
CTGGAGTCTCGGCTCCGGACTGTTA345700.20857297052375828No Hit
TGAAAATCTTTGCTCTGAAGTGG344050.20757746748249647No Hit
TCTCGGGCTGAGACTTGAAGCA338530.2042470573080934No Hit
TATTAATGCTGTAGGTGAAAGACCA320520.19338099077892684No Hit
GGATCCGGCCCGGACTGGCCGTGGT306880.1851514989711627No Hit
CTTTCCGAGGTCGCGGTCGGACCGG304350.18362506097456127No Hit
CGGACTGGCCGTGGTCGAGGCGAGG297240.17933534786948774No Hit
TGAAAATCTTTGCTCTGAAGTGGGA277000.16712384389667645No Hit
TGAAAATCTTTGCTCTGAGGTGGAA266160.16058369058317473No Hit
CTCGGGCTGAGACTTGAAGC260300.1570481464487541No Hit
CTCGGGCTGAGACTTGAAGCGGTTG253860.15316266791195046No Hit
GACCAAGATAGTCGAACTCGACCCT251830.1519378975036102No Hit
GGACCGGGAAGGAATCGGCCGTGGA247930.14958489031517325No Hit
GGAAGGAATCGGCCGTGGATTGGCC234190.14129506498975689No Hit
CGGACTGGCCGTGGTCGAGGC232750.14042626233556477No Hit
TCCCTTTCCGAGGTCGCGGTA224760.1356056142751516No Hit
AGGAATCGGCCGTGGATTGGCCCAG224100.1352074130586469No Hit
CCCTTTCCGAGGTCGCGGTA222520.13425414347974166No Hit
TAGAATGTTTACTCGTGAAGGAAAA221860.13385594226323694No Hit
CTTTCCGAGGTCGCGGTA221320.1335301412679149No Hit
TTGCTACGATCTTCTGAGATTAAGC215300.12989806350525068No Hit
GGATCCGGCCCGGACTGGCT203320.12267010809051354No Hit
CGAAAATCTTTGCTCTGAAGTGGAA201980.12186163895397367No Hit
GTTGGATCCGGCCCGGACT200720.12110143663155559No Hit
TGATCATGGATTCTAAACTGGTAAT195600.11801236052776141No Hit
CTCCCTTTCCGAGGTCGCGGTA189460.11430788254391451No Hit
GACCAAGATAGTCGAACTCGAC189450.1143018491921493No Hit
TGGATCCGGCCCGGACTGGCC188350.11363818049797475No Hit
TTTCTGCTGAACTACTTTGAGAACG181570.10954756800115359No Hit
GGAGTCTCGGCTCCGGACTGTTACA180170.10870289875402235No Hit
GGTGCAGGAATTAACTGAGTTGGAC179770.10846156468341343No Hit
GCGACTGCTGCCGGTCGTTGGGGCG178220.10752639515980388No Hit
GTTGGATCCGGCCCGGACTGGCT174890.10551728902198462No Hit
ACCCGTAGATCCGAACTTGTGA170860.10308584826059977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)