FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-47-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-47-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15063975
Total Bases365.1 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA221072414.675568699496647No Hit
TCGGACTGTAGAACTCTGAACGTGT6979084.632960423792524Illumina RNA PCR Primer (100% over 25bp)
ATAGACACTCAGACAGACATGCTCC6674764.430942032232528No Hit
GGCGAGAATTCTACCACTGAACCAC4117912.7336144676288963No Hit
TGCGAGAATTCTACCACTGAACCAC1486530.9868112500186703No Hit
TTCCACTTCAGAGCAAAGATCTTCA1394240.9255458801544745No Hit
GAGAATTCTACCACTGAACCACCAG1267100.8411458462988688No Hit
TGACAGGGTCGAGTTCGACTATCTT1004200.6666235173651045No Hit
ACAAGTTCGGATCTACGGGT989830.6570842025428215No Hit
AAACTAACCAGCCTAGACTTGA945690.6277825076050644No Hit
AACCGCTTCAAGTCTCAGCCCGAG817710.5428248520061936No Hit
AGCGAGAATTCTACCACTGAACCAC789830.5243171208130657No Hit
GACCGCGACCTCGGAAAGGG729420.48421482377659286No Hit
GACCGCGACCTCGGAAAG720370.4782071133283214No Hit
AGGCGAGAATTCTACCACTGAACCA618140.41034321950215663No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG615140.4083517132762103No Hit
GTAGACACTCAGACAGACATGCTCC590910.3922669813246503No Hit
GACCGCGACCTCGGAAAGGGAG586070.3890540179467903No Hit
AGAATTCTACCACTGAACCACCAGT521960.346495529898317No Hit
CCACAAGTTCGGATCTACGGGT510640.33898091307241285No Hit
GCCAGAATTGCCTGAAGCCGTGCAG501970.33322546007942794No Hit
ATCCACTTCAGAGCAAAGATTTTCA501690.33303958616500623No Hit
CGCTTCAAGTCTCAGCCCGAG459520.3050456469822872No Hit
TCCACTTCAGAGCAAAGATTTTCA450720.2992038953861779No Hit
CCAGAATTGCCTGAAGCCGTGCAGG405050.2688865322731882No Hit
ACAAATCTTAGAACAAAGGCTTAAT402580.2672468588138257No Hit
GAGAATTCTACCACTGAACCACAGT400090.26559390864629023No Hit
GACGTACTGTCGTCAGCGTTCAAAT370840.24617672294331344No Hit
TGCTTCAAGTCTCAGCCCGAG368030.24431134544501037No Hit
GCCAGTCCGGGCCGGATCC341120.22644753459827172No Hit
TGGCGAGAATTCTACCACTGAACCA324200.21521543948393435No Hit
CTCCACTTCAGAGCAAAGATTTTCA315140.2092010906815764No Hit
AGAGAGAACCCCCCCTCGCCTCGAC274690.18234894840173327No Hit
GCAAGTTCGGATCTACGGGT274370.18213652107096567No Hit
AACCGCTTCAAGTCTCAGCCCGAGA257650.17103719303835807No Hit
TTCCACCTCAGAGCAAAGATTTTCA257540.1709641711434067No Hit
TTTTTTTGGTCTTTCACCTACAGCA246890.1638943240412972No Hit
TCAGAATTGCCTGAAGCCGTGCAGG244570.16235422589323203No Hit
TGCGTGAGCCTAGAGATCCACCGTC240020.15933377478388008No Hit
TCACAAGTTCGGATCTACGGGT235690.15645936746443087No Hit
CCACTTCAGAGCAAAGATTTTCA228360.1515934539190353No Hit
GGCCAGTCCGGGCCGGATCC223520.14838049054117522No Hit
TTCCCGGTCCGACCGCGACCTCGGA221170.14682047733085057No Hit
GTACTGTCGTCAGCGTTCAAATATG220040.14607034331907748No Hit
GACCGCTTCAAGTCTCAGCCCGAG215510.1430631689178985No Hit
GGGCGAGAATTCTACCACTGAACCA213750.14189481859867664No Hit
GTCGTCAGCGTTCAAATATGGAAAC212690.14119115306550895No Hit
GAACTAACCAGCCTAGACTTGA210030.1394253508785032No Hit
TCCCACTTCAGAGCAAAGATTTTCA207830.13796491297947586No Hit
TGTACTGTCGTCAGCGTTCAAATAT201190.13355704586604797No Hit
ACTCGTTCTCAAAGTAGTTCAGCAG196240.1302710605932365No Hit
AAATCCATAGAATCTCAGCAGTT192880.1280405736201766No Hit
GACCCGAGGGTCACAGCTG184950.12277635882959179No Hit
GACGTACTGTCGTCAGCGTTCAATA184670.12259048491517013No Hit
GGCCAATCCACGGCCGATTCCTTCC184640.12257056985291066No Hit
GACAGGGTCGAGTTCGACTATCTTG177640.11792372199236922No Hit
AGATCCACCGTCAAAAGTT164720.10934696851262699No Hit
AGAATTCTACCACTGAACCACAGTG163480.10852381260590249No Hit
TGTGAGATAACCCTCAGCCGTCCGG163160.10831138527513488No Hit
ATTGGTGAGATGGTGGGACTGA155210.10303389377637709No Hit
GCCTCTACTGCAGTCCAGGGCAATT152110.10097600400956587No Hit
GACCGCGACCTCGGAAAGGGA151540.10059761782663607No Hit
CCTTCCTTAGGGAGTTACACAA150780.10009310291606299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)