FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-47-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-47-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15063975
Total Bases365 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA223176714.815259584538609No Hit
GCACTGGTGGTTCAGTGGTAGAATT7958155.282901757338285No Hit
AACTTTTGACGGTGGATCTCTAGGC6968354.625837469857723No Hit
GGAAGAGCACACGTCTGAACTCCAG6480204.301786215125822Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTC2493901.6555391256291914No Hit
TTCCTGCACGGCTTCAGGCAATTCT1431730.9504330696247173No Hit
TGAAGATCTTTGCTCTGAAGTGGAA1406050.9333857763306166No Hit
TTTCCATATTTGAACGCTGACGACA1347440.8944783830297115No Hit
CGACCAAGATAGTCGAACTCGACCC1307240.8677921996020306No Hit
CAACTTTTGACGGTGGATCTCTAGG1028430.6827082493166645No Hit
ACCCGTAGATCCGAACTTGT1015120.6738726000275492No Hit
TCAAGTCTAGGCTGGTTAGTTT955770.6344739685242441No Hit
CTCGGGCTGAGACTTGAAGCGGTT827370.5492375020537408No Hit
CCCTTTCCGAGGTCGCGGTC751430.49882584112095246No Hit
CTTTCCGAGGTCGCGGTC743220.493375752415946No Hit
CTCCCTTTCCGAGGTCGCGGTC605830.4021714056216902No Hit
ACCCGTAGATCCGAACTTGTGG537890.3570704279580921No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC521170.34597109992548447No Hit
TGAAAATCTTTGCTCTGAAGTGGAT493730.32775545631216196No Hit
CTCGGGCTGAGACTTGAAGCG467750.3105090123954667No Hit
TGAAAATCTTTGCTCTGAAGTGGA459340.30492615660873046No Hit
TATTAATGCTGTAGGTGAAAGACCA453910.30132153033976755No Hit
CTCGGGCTGAGACTTGAAGCA368820.24483577541784288No Hit
GGATCCGGCCCGGACTGGC351690.2334642748676893No Hit
TAGGAATTGCCCTGGACTGCAGTAG336010.22305533566007643No Hit
TGAAAATCTTTGCTCTGAAGTGGAG317830.21098680793084162No Hit
TCTCGGGCTGAGACTTGAAGCGGTT314760.20894883322628988No Hit
TAAGAAATCTTCCACTGATCGTTGA314410.20871649083326282No Hit
TCTGTTTCCATATTTGAACGCTGAC295490.1961567249016279No Hit
CACCCCTCATCCGCTACTTTGACCT292180.19395942969900043No Hit
TTCATTCTGACAGACGCTTTGTAAA284960.18916653804855624No Hit
ACCCGTAGATCCGAACTTGC282080.18725469207164774No Hit
TTCTGCTGAACTACTTTGAGAACGA280020.18588719112983126No Hit
TGATATCTCTTGATCTTTGACGTGT266730.177064818548889No Hit
GTGGTCCGGACGGTCGAGGGTTATC266290.17677273096908352No Hit
GCACTTGTGGTTCAGTGGTAGAATT263150.17468828778592635No Hit
TCCCGATCGTTGTTAAAGTCTTAGA261780.17377883327607752No Hit
TGAAAATCTTTGCTCTGAGGTGGAA261120.17334070190636933No Hit
TTTCCATATTGAACGCTGACGACAG257930.17122306695277972No Hit
CCCTTTCCGAGGTCGCGGTCGGACC254190.16874032252443327No Hit
TGTGACTTCTCTATCCGCACGCGGC251470.16693469021290863No Hit
ACCCGTAGATCCGAACTTGTGA244710.16244716285044286No Hit
CACCAAGGTCTTGTAGTCCAGTACA236440.15695724402091743No Hit
TTGCTACGATCTTCTGAGATTAAGC235200.15613408811419296No Hit
TGAAAATCTTTGCTCTGAAGTGG231750.15384385595435468No Hit
CTTTCCGAGGTCGCGGTCGGACCGG229580.15240333311758683No Hit
GGATCCGGCCCGGACTGGCC228660.15179260454162996No Hit
TAGAATGTTTACTCGTGAAGGAAAA228090.15141421835870014No Hit
GGCCGAGCTGTTCTTCCTCGCCGAG224560.14907087936616997No Hit
GAGGTCCGGACGGCTGAGGGTTATC224230.14885181368131586No Hit
CTCGGGCTGAGACTTGAAGCGGTC218980.14536667778590975No Hit
TGAAAATCTTTGCTCTGAAGTGGGA214640.14248563211237406No Hit
TCAAGTCTAGGCTGGTTAGTTC213710.1418682651823307No Hit
TCCGAAGCCTCGTTTGTAGTCTTCC212640.14115796129507652No Hit
TCAGACTCACTGAAGGTACCGCTTG209270.13892083596793012No Hit
ACACTGGTGGTTCAGTGGTAGAATT201420.1337097280100372No Hit
CTCGGGCTGAGACTTGAAGCGGTTG199820.13264759135619913No Hit
TGGAACAACCTTTCGACTGACGATC197850.13133983560116105No Hit
CAGCTGTGACCCTCGGGTC194430.12906951850358223No Hit
AACTGCTGAGATTCTATGGATTT193870.1286977706747389No Hit
GGTGCAGGAATTAACTGAGTTGGAC188890.12539187033966798No Hit
TACAATGATCTTTCACGTCTTTTAG185570.12318793678295405No Hit
TATAGACTTGGAAGCGGGAAAAAAA183060.12152170990724559No Hit
TTCATTCTAACAGACGCTTTGTAAA180140.11958331051399117No Hit
TTTGTAGGCTCTAACAGTACATTGT176260.1170076291284339No Hit
AGCATTTCCCGAAGGTCTGACATAC174120.11558702135392551No Hit
GATACGATCCACTGAGATTCATCCT170430.11313746869601152No Hit
AACTTTTGACGGTGGATCT168900.11212180052077889No Hit
TGATGACTCTTTTCTATTCTGACGA167000.11086051324434619No Hit
GGACTGGCCGTGGTCGAGGCGAGGG166910.1108007680575678No Hit
CGGACCTCAGATCAGGCAAGGCTAC163250.10837113046191327No Hit
GTGGTCGAGGCGAGGGGGGGTTCTC160700.10667835016985888No Hit
TCGTTCTTTTTCATACTTCTGAAGC159230.10570251211914516No Hit
TCAGTCCCACCATCTCACCAAT158750.10538387112299377No Hit
TGATCATGGATTCTAAACTGGTAAT158460.1051913588544856No Hit
TGATCTTCTTGAATTTGCCCATTTA156560.10393007157805294No Hit
TCCCTTTCCGAGGTCGCGGTC156080.10361143058190152No Hit
TGCGTTTCTGGTAGACTTATGTTTT155550.10325959781531766No Hit
TGTGCTAGAATTAACTGAGTTGGAG153330.10178588320811739No Hit
TTGTGTAACTCCCTAAGGAAGG152910.1015070723364849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)