FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-57-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-57-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26459749
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG18430806.965598955606117Illumina RNA PCR Primer (100% over 36bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG17107476.465469494816448Illumina RNA PCR Primer (100% over 37bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG9186423.471846992955224Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA6712042.5366982884078Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG4436051.6765276193663061Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG3001581.1343947366998832No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG2311070.8734285423493624Illumina Small RNA Adapter 1 (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1937160.7321157883999581No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG1739270.6573267191612437Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1601760.6053572163515232Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1591970.6016572568394356Illumina RNA PCR Primer (100% over 36bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC1564230.5911734083342968Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1545440.5840720560123227Illumina RNA PCR Primer (100% over 50bp)
GACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1545030.5839171036732056Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1449400.54777541540549Illumina RNA PCR Primer (100% over 37bp)
TAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1442260.5450769771096469Illumina RNA PCR Primer (100% over 36bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG1378970.5211576270054564Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC1326730.5014144314067379Illumina RNA PCR Primer (100% over 32bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT1288850.48709834700246024Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1235600.4669734395439654Illumina RNA PCR Primer (100% over 37bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG1202310.4543920654727299Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC1102770.41677266099538585Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1066170.402940330235181Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT990150.37420989896767354Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC910120.3439639582370944Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG896150.3386842407310818Illumina Small RNA Adapter 1 (100% over 26bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT772890.29210027653701476Illumina RNA PCR Primer (100% over 31bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC719540.2719375758250768Illumina RNA PCR Primer (100% over 32bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT719380.2718771066195677No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA698110.26383848161220275Illumina RNA PCR Primer (100% over 45bp)
CAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG685420.2590425177502628Illumina RNA PCR Primer (100% over 36bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC671100.2536305238571991Illumina RNA PCR Primer (100% over 30bp)
AGCTGCTCCCACTTATTCTACACCTCGATCGTCGGACTGTAGAACTCTGA660340.24956396978671266Illumina DpnII expression Sequencing Primer (100% over 24bp)
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT639610.24172942834794087Illumina RNA PCR Primer (100% over 35bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG622640.23531591323863277Illumina Small RNA Adapter 1 (100% over 26bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT604570.22848667234144968Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC604420.22842998246128485Illumina RNA PCR Primer (100% over 32bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT599610.2266121269706678Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT597800.22592806908334617No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC572390.21632480338343346No Hit
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG559070.2112907420248015Illumina Small RNA Adapter 1 (100% over 26bp)
AGCCTTAACTCCAGAAAGTGGATCGTCGGACTGTAGAACTCTGAACGTGT543710.20548569829592866Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTGA536380.20271545281854336Illumina DpnII expression Sequencing Primer (100% over 24bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG508280.19209554860100903Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA466560.1763282032645132Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG457080.17274540283809947Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC450510.17026238608688238Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGT449120.16973705986402216Illumina DpnII expression Sequencing Primer (96% over 28bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG444960.16816486052078575Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG440620.1665246333213516Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT436560.1649902272315584Illumina RNA PCR Primer (100% over 35bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT426460.16117310863379694Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAGGATCGTCGGACTGTA420130.15878079569084347No Hit
GCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAACG378020.14286605666591926Illumina Small RNA Adapter 1 (100% over 26bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC376030.1421139709223999No Hit
TAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA367450.13887130977697482Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA366680.13858030172546232Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC362220.1368947226218964Illumina RNA PCR Primer (100% over 32bp)
TGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT358030.13531118530262704Illumina RNA PCR Primer (100% over 31bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT343000.12963085931011667Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC341700.1291395470153553Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT337340.1274917611652325Illumina RNA PCR Primer (100% over 35bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA324910.12279405976224492Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG321730.12159223430275169No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAACTC316820.11973658555869143Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG313370.11843271831490164Illumina DpnII expression Sequencing Primer (100% over 23bp)
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA312130.11796408197220615Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG309190.1168529603209766Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTA305440.11543571331685724Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCTTAACTCCAGAAAGTGAGATCGTCGGACTGTAGAACTCTGAACGTG301640.1139995696860163Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGT296120.11191338209595261Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC294680.11136915924637078Illumina RNA PCR Primer (100% over 30bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT291960.11034118275271622Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT287730.10874252813206958Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT285270.10781281409736729Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)