FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-48-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-48-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24884997
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG384795315.462943395170994Illumina RNA PCR Primer (100% over 37bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA303353312.190208421564206Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT9514963.8235728941417997Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG5889722.366775451088059Illumina RNA PCR Primer (100% over 36bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG5575312.240430247992395No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT4142541.6646736987752098Illumina RNA PCR Primer (100% over 50bp)
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG3537641.4215955099371722Illumina RNA PCR Primer (100% over 37bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG2993681.2030059718311399Illumina RNA PCR Primer (100% over 37bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG2275120.9142536766229066Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1751200.7037171834901166No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1668450.6704642158486095Illumina RNA PCR Primer (100% over 45bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG1599050.642575926370415Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG1343230.5397750299105923Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCGCGACCTCGGAAAGGGGATCGTCGGACTGTAGAACTCTGAACGTGT1121000.4504722262976363Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGACCTCGGAAAGGGAGATCGTCGGACTGTAGAACTCTGAACGTG1111610.44669886839849726Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG1077560.43301592521791343Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGACCTCGGAAAGGGAGGATCGTCGGACTGTAGAACTCTGAACGT905910.3640386213428115Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG905770.36398236254559324Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT815180.32757890225986364Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC779150.313100298947193Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG741010.29777379519073277Illumina Small RNA Adapter 1 (100% over 26bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC709190.2849869742801255Illumina RNA PCR Primer (100% over 32bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT707080.284139073836336No Hit
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT674190.2709222749755606Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC664080.2668595861192991Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC637960.25636330195257806No Hit
TAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG565060.22706854254392717Illumina RNA PCR Primer (100% over 36bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA564860.22698817283361536Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT535560.21521401027293674Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGACCTCGGAAAGGGATCGTCGGACTGTAGAACTCTGAACGTGTA512750.20604784481187602Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGTA487070.19572837400784093Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA477880.19203538581901375Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT469040.18848304462323223Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG463550.18627689607517334Illumina RNA PCR Primer (100% over 37bp)
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT458820.18437615242629926Illumina RNA PCR Primer (100% over 35bp)
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG456320.18337153104740178Illumina RNA PCR Primer (100% over 36bp)
GTAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG455610.18308621857579488No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT429590.17263011926422978No Hit
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT400200.16081979033391083Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT398210.16002011171630842Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG380170.1527707638461841Illumina Small RNA Adapter 1 (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG361830.1454008614105921No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG360560.14489051375011217Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTGA358300.14398233602358881Illumina DpnII expression Sequencing Primer (100% over 24bp)
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA347790.13975890774670377Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA329010.1322121919484258Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG320550.12881255320223667Illumina Small RNA Adapter 1 (100% over 26bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC319900.12855135164372333No Hit
TGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT316690.12726141779321895Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGAATTCTACCACTGAACCACAAGTGCGATCGTCGGACTGTAGAACTCT298070.11977899776319041Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCGCGACCTCGGAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA296150.11900744854419713Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT286900.11529034944227641Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG283780.11403658196141232Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG266780.10720515658490938Illumina RNA PCR Primer (100% over 37bp)
CAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG266550.1071127314180508Illumina RNA PCR Primer (100% over 37bp)
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA254140.10212579089320364Illumina DpnII expression Sequencing Primer (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)