FastQCFastQC Report
Fri 9 Feb 2024
POR-82-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-82-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13933812
Total Bases368 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5307153.808828481394754No Hit
TGAAAATCTTTGCTCTGAAGTGGAA4004122.873671612621155No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2662731.9109845891418658No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT2639051.8939899576655692No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT1908391.3696108430341962No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT1247040.8949740386909197No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT1142180.8197182508275552No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC900390.646190719381028No Hit
TCAGGTCTAGGCTGGTTAGTTT795980.5712578869300088No Hit
TCCCGTAGATCCGAACTTGT793100.5691909722909998No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC697180.5003512319528928No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT452700.32489314481923537No Hit
TTACCGTGACCGATCCCGT437370.3138911304386768No Hit
CTGGCAACCTACCGCTAGTGGATGTGT429340.30812817052505087No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT427530.30682917208872923No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC404470.2902795014027748No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC401290.2879972831555356No Hit
TGAAAATCTTTTCTCTGAAGTGGAA384440.27590439715994447No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC383400.27515801131808004No Hit
GAGGTCCGGACGGAGGAGGGTTAT356630.2559457526770133No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC334420.24000610888104418No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT301940.21669590489666432No Hit
TCTCAGCTCACCAATCTCTGCT297920.21381083654638086No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT279630.20068449323128518No Hit
CACCCCTCTTCCACTAACTTGACCTCAT258410.18545535134247543No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC253920.18223297400596478No Hit
GAGGTCCGGACGGTTGAGGGTTATC245780.1763910694359878No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA219840.15777448411102432No Hit
TGACCACTTTGGATTCTTGCGCCATTCTT217010.1557434534067203No Hit
CACCCCTCTTCCACTAACTTGACCTCATT212620.1525928439396197No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG192350.13804549681020525No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG192030.13781583962809316No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT190560.1367608519477656No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT171440.12303883531656665No Hit
TGGATTTACAAACTTTCATAATGTCTGT170730.12252928344375538No Hit
GTGCTTAGGGTACTGTCGGCT169950.12196949406235709No Hit
TCAAGTCTAGGCTGGTTAGTTT166720.1196513918804129No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGT158140.1134937086850318No Hit
TATCAGCATCTGTTCAGCATCTGACGTGT154570.11093159574709348No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACC149360.10719248975083057No Hit
TGAAAATCTTTGCTCTGAAGTGGA148140.10631692174402811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)