FastQCFastQC Report
Fri 9 Feb 2024
POR-79-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-79-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12259576
Total Bases324.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5101064.160877994475502No Hit
TGAAAATCTTTGCTCTGAAGTGGAA4428453.6122374868429383No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2513042.0498588205660617No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT2078391.6953196423758865No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT1817711.4826858612402256No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC1163580.9491192843863442No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT665320.542694135588376No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT623230.5083617899999152No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC612030.49922607437647115No Hit
GTTCAAGATAGTCGAACTCGACTCTGTT610100.4976517948092169No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT574390.4685235443705394No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC510790.4166457306516963No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG468530.3821747179510939No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC458680.37414018233583285No Hit
TCAGGTCTAGGCTGGTTAGTTT442780.361170728906122No Hit
CGACTTGAGCCCCTGACGAAGTAGGCT439330.3583566022185433No Hit
TCCCGTAGATCCGAACTTGT395650.3227273112871114No Hit
GTTCAAGATAGTCGAACTCGACTCTGT386290.315092463230376No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT383090.31248225876653485No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG381110.3108671947545331No Hit
TGAAAATCTTTTCTCTGAAGTGGAA319900.26093887749462136No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC318670.2599355801538324No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT310110.25295328321305727No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC308440.2515910827584902No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC300950.24548157293531195No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT286160.23341753417899608No Hit
CACCCCTTCTCCAAATACTAGACCTCTT277310.22619868745868535No Hit
CACCCCTCTTCCACTAACTTGACCTCAT265800.2168101082778067No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT262960.21449355181614765No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT240750.19637710145930004No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT237800.19397081921919648No Hit
CACCCCTCTTCCACTAACTTGACCTCATT216740.17679241109154184No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA213230.17392934307026603No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGT192270.15683250383210645No Hit
TGGGAATACCCCGTGTTGTAGGCTT175630.14325944062013238No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA172920.1410489237148169No Hit
TTGTGTGATTCCATCTTCTTGGTGT165740.13519227744907328No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGT165230.1347762761126486No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT162740.1327452107642222No Hit
CACCCCTTCTCCAAATACTAGACCTCTTT159060.12974347563080485No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT155610.12692934894322608No Hit
TGAAAATCTTTGCTCTGAAGTGGA152410.12431914447938494No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC148610.12121952667857355No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTT146880.11980838489030943No Hit
TCAAGTCTAGGCTGGTTAGTTT146760.11971050222291538No Hit
GAGGTCCGGAACTCAGGGGAGGGTT145150.1183972431020453No Hit
CAATGTTTCGGCTTGTTCCCG140020.11421275907094992No Hit
GAGGTCCGGACGGAGGAGGGTTAT139350.11366624751133318No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCGT136550.11138231860547217No Hit
GTTCAAGATAGTCGAACTCGACCCTGT135000.11011800081829909No Hit
CACCCCTTCTCCAAATACTAGACCTCT132420.10801352346932716No Hit
TCTCAGCTCACCAATCTCTGCT131530.10728756035282133No Hit
CTGGCAACCTACCGCTAGTGGATGTGT131490.10725493279702333No Hit
AACTTTTGACGGTGGATCT123650.10085993186061248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)