FastQCFastQC Report
Fri 9 Feb 2024
POR-73-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-73-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12238952
Total Bases309.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA11960109.772160230712565No Hit
CTCGGGCTGAGACTTGAAGCGG2927412.3918796315240063No Hit
CTCGGGCTGAGACTTGAAGCGGGT2881792.3546051982228544No Hit
TCTCGGGCTGAGACTTGAAGCGGGT2233001.8245026208126316No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1772031.447860895279269No Hit
GTGGAGCCGGCCTGGGCTG1006010.8219739729349376No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT774110.6324969654264515No Hit
CTCGGGCTGAGACTTGAAGCG769580.628795668125833No Hit
CGGGCTGAGACTTGAAGCGGGT753980.6160494787462194No Hit
TATTAATGCTGTAGGTGAAAGACA651000.5319082875723347No Hit
GTGGAGCCGGCCTGGGCT595660.48669199781157735No Hit
TCTCGGGCTGAGACTTGAAGCGG565510.46205753564520885No Hit
TCCCGTAGATCCGAACTTGT551110.4502918223717194No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC525600.42944853448236414No Hit
CAATGTTTCGGCTTGTTCCCG471850.38553137556222133No Hit
CTCGGGCTGAGACTTGAAGCGGGTG432700.35354334259992193No Hit
CACCCCTCTTCCACTAACTTGACCTCAT386710.3159665958327151No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT386630.3159012307589735No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG350500.2863807293304198No Hit
TCAGGTCTAGGCTGGTTAGTTT349660.2856943960561329No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT343120.28035080127775647No Hit
TGAAAATCTTTGCTCTGAAGTGGA341650.27914971804775446No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA310820.25395965275458227No Hit
CTCGGGCTGAGACTTGAAGCGGGC302570.24721887952497892No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTA286200.23384355131060242No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT277320.22658802812528392No Hit
CACCCCTCTTCCACTAACTTGACCTCATT268160.2191037271818698No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT264110.21579462032370092No Hit
CGGGCTGAGACTTGAAGCGG254460.20790995830361944No Hit
CTCGGGCTGAGACTTGAAGCGA253510.20713374805293788No Hit
TGAAAATCTTTGCTCTGAAGTGG238720.1950493800449581No Hit
TCTCGGGCTGAGACTTGAAGCGGGC235120.19210795172658574No Hit
CACCCCTCCTCCAAATACTTGACCTCTT221300.18081613523772297No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA212320.17347890571022748No Hit
CTGGCAACCTACCGCTAGTGGATGTGT211620.17290696131498842No Hit
CACTAACTTGACCTCATT203390.1661825293538205No Hit
TGCCTAAAATCGTCAGGAGGTTGAATAGC187320.15305232016597498No Hit
GAGACTTGAAGCGGGT184590.15082173702454263No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC181810.14855030071202174No Hit
TTACCGTGACCGATCCCGT179580.14672824928147443No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC179300.1464994715233788No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA172990.14134380133200947No Hit
TCTCGGGCTGAGACTTGAAGCG172270.14075551566833502No Hit
TAACGGTTTGTTTCTTCCACAATG166840.13631886128812337No Hit
TATCAGCATCTGTTCAGCATCTGACGTGT163120.1332793853591386No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC160420.13107331412035933No Hit
TGAAAATCTTTGCTCTGAGGTGGAA149830.12242061248381396No Hit
TGACGATCTTCCATTGTTCTTCGGAATGA147340.12038612456360644No Hit
TCGGGCTGAGACTTGAAGCGGGT143710.11742018434258096No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT137620.11244426810400107No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT133950.10944564534610479No Hit
CCCCCCTCCCCGCTCTTAGACCTCAC131110.10712518522827771No Hit
TCTCGGGCTGAGACTTGAAGCGGGTG130810.10688006620174668No Hit
GAGATTAAGCCTTTGTTCTAAGATTTGT127770.10439619339956559No Hit
TGAGATTAAGCCTTTGTTCTAAGATTTGT123990.10130769366527462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)