FastQCFastQC Report
Wed 15 Nov 2023
sRNA-POR-82-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-82-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17260661
Total Bases416.7 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCAC6400633.708218358497395No Hit
TTCCACTTCAGAGCAAAGATTTTCA3982262.307130648125237No Hit
TCGGACTGTAGAACTCTGAACGTGT3357911.9454121716427895Illumina RNA PCR Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCAC3263331.8906170511082978No Hit
ACAAATCTTAGAACAAAGGCTTAAT3003911.7403215322982126No Hit
AGACAACTACTCACGCTTAAGCACA2710931.570582957396591No Hit
AGCAGACAACTACTCACGCTTAAGC1991651.1538665871486613No Hit
AAGGATGATAACCCTCGACCGTCCG1309560.7586963210736831No Hit
AGTAGATAACCCTCCCCAAGTCCGG1192550.6909063331931494No Hit
AAAGAGGTCAAGTATTTGGAGGAGG1191770.6904544385640852No Hit
AGGCGAGAATTCTACCACTGAACCA1183700.6857790672095351No Hit
TCCGACACTCAGACAGACATGCTCC868940.5034222038194249No Hit
AAGCCTACAACACGGGGTATTCCCA855140.4954271449975177No Hit
AAACTAACCAGCCTAGACCTGA795690.46098466333357685No Hit
ACAAGTTCGGATCTACGGGA792230.4589801051072146No Hit
GTGGATAACCCTCCTCCGTCCGGAC708840.4106679344435303No Hit
AAGAGGTCAAGTATTTGGAGGAGGG693310.40167059650844195No Hit
AATGAGGTCAAGTTAGTGGAAGAGG692760.40135195285974273No Hit
AATGAGGTCAATGAAGGCGGGGAGG685090.39690832234061024No Hit
AGAATCGTGGATAACCCTCCTCCGT581090.336655705132034No Hit
AGCCGACAGTACCCTAAGCACTTCC475930.2757310395007468No Hit
ACACATCCACTAGCGGTAGGTTGCC467410.2707949597063519No Hit
ATGAGGTCAAGTTAGTGGAAGAGGG451060.2613225530586575No Hit
GCTCTTCTAGGTGCAGGAGTCGACA450880.26121826968271955No Hit
ACACTCTTCTTGGTGCAGGAGTCGA442700.25647916959842965No Hit
ACGGGATCGGTCACGGTAA436510.25289298017034223No Hit
GGACAACTACTCACGCTTAAGCACA409430.2372041256125707No Hit
AATCGTGGATAACCCTCCTCCGTCC405690.23503734880141613No Hit
AAACACAGCAGACAACTACTCACGC398930.23112092868285866No Hit
GCGAGAATTCTACCACTGAACCACC390820.22642238324476682No Hit
GCAAATCTTAGAACAAAGGCTTAAT388830.22526947258856422No Hit
TTCCACTTCAGAGAAAAGATTTTCA382110.22137622655354858No Hit
AGTGAGGTCTAAGAGCGGGGAGGGG377300.21858954300765193No Hit
ATAACCCTCCTCCGTCCGGACCTC356130.2063246592931754No Hit
AGTAGTAGATAACCCTCCCCAAGTC348640.20198531214998083No Hit
GGCAGACAACTACTCACGCTTAAGC343610.1990711711446045No Hit
AGGATGATAACCCTCGACCGTCCGG314360.18212512255469243No Hit
GAATCGTGGATAACCCTCCTCCGTC309720.17943692886384827No Hit
AGCAGAGATTGGTGAGCTGAGA296180.17159250158496248No Hit
ATCAGAGGACAATTTCACATTCCTC291590.1689332754985455No Hit
AGTAGATAACCCTCCCCAAATCCGG289210.16755441752781078No Hit
AGTCTCATGCTCTACCGACTGAGCT274990.15931603082871507No Hit
TGCGAGAATTCTACCACTGAACCAC273940.1587077111357439No Hit
GCTGAGTTCATCAACAGAATATTTA258070.14951339349055057No Hit
GATAACCCTCAACCGTCCGGACCTC244140.14144301889713262No Hit
AATGAGGTCAAAGAGCGGGGAGGGG239730.13888807618665358No Hit
AAAAGAGGTCAAGTATTTGGAGGAG238950.13843618155758924No Hit
GTCTCATGCTCTACCGACTGAGCTA236060.13676185402169708No Hit
AGCCTTCATAAATTCCATCATCAAC228330.13228346237725194No Hit
ACTCTTCTTGGTGCAGGAGTCGACA226140.1310146813033406No Hit
ATAGAGGTCAAGTATTTGGAGGAGG225620.1307134182172977No Hit
AAGAATGGCGCAAGAATCCAAAGTG220490.12774134200306697No Hit
ATCATCAGAGGACAATTTCACATTC220150.127544362292962No Hit
GGGCGAGAATTCTACCACTGAACCA200430.1161195391068743No Hit
CGTGGATAACCCTCCTCCGTCCGGA198080.1147580616987959No Hit
CGCTCTTCTAGGTGCAGGAGTCGAC197520.11443362452921126No Hit
ACGAGAATTCTACCACTGAACCACC196490.11383689187801094No Hit
AATTCAGAAAAACTGAACAGTCATC193060.11184971421430501No Hit
AAGTAGTAGATAACCCTCCCCAAGT178550.10344331540953153No Hit
GCTTTAAGAAAAGACACACCTCACA177930.10308411711463425No Hit
AAATGAGGTCAATGAAGGCGGGGAG177670.10293348557161282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)