FastQCFastQC Report
Wed 15 Nov 2023
sRNA-POR-82-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-82-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17260661
Total Bases416.6 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATT10195945.907039133669331No Hit
GTGTGCTTAAGCGTGAGTAGTTGTC6224893.6064030224566714No Hit
TGAAAATCTTTGCTCTGAAGTGGAA4023692.331133205153615No Hit
GGAAGAGCACACGTCTGAACTCCAG3116361.8054696746549854Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTCCGGACGGAGGAGGGTTATC2991891.7333577201939139No Hit
GAGGTCCGGACTTGGGGAGGGTTAT2684211.5551026695906953No Hit
GAGGTCCGGACGGTCGAGGGTTATC2153001.247345046635236No Hit
GCACTGTGGTTCAGTGGTAGAATTC1745411.0112069288655863No Hit
GTTGCTACGATCTTCTGAGATTAAG1248930.7235702039452603No Hit
TTGCTACGATCTTCTGAGATTAAGC1171880.6789311255229449No Hit
AACTTTTGACGGTGGATCTCTTGGC1143650.6625760160633477No Hit
CTTCGGTACGCCCCTCCCCGCCTTC1045190.6055330094253053No Hit
AGCTGTCGACTCCTGCACCTAGAAG958500.5553089768694258No Hit
AGCTGTCGACTCCTGCACCAAGAAG841560.48755954363509024No Hit
GATTCTATAGACACCCCTCCTCCAA819460.4747558624782678No Hit
TCAGGTCTAGGCTGGTTAGTTT794310.46018515745138616No Hit
TCCCGTAGATCCGAACTTGT790660.4580705223282005No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC775840.4494845243759784No Hit
GGTGACCGCCTGGGAATACCCCGTG638320.3698120251594073No Hit
CACCCCTCTTCCACTAACTTGACCT621600.3601252582389516No Hit
GAGGTCCGGACGGTTGAGGGTTATC613330.35533401646669266No Hit
CTGGCAACCTACCGCTAGTGGATGT505600.29292041596784735No Hit
GAGGTCCGGATTTGGGGAGGGTTAT489460.28356967325874716No Hit
GGGGACTAGTTGGGAAGTGCTTAGG442540.25638647326426256No Hit
TTACCGTGACCGATCCCGT436720.25301464410893654No Hit
GAGGTCCGGAACTCAGGGGAGGGTT423140.24514704274650898No Hit
TGAAAATCTTTTCTCTGAAGTGGAA387820.22468432697913482No Hit
GAGGTCCGGACGGAGGAGGGTTAT355930.2062087888754666No Hit
TGACTGTTAGTGAGGAATGTGAAAT353920.20504429117749315No Hit
TTGTTTAAATATTCTGTTGATGAAC351240.20349162758019523No Hit
CACCCCTCCTCCAAATACTTGACCT335320.19426834233057472No Hit
GCCTACGACCATACCACAGGGAGAA330370.19140054949228189No Hit
TATTAATGCTGTAGGTGAAAGACCA312750.1811923656921366No Hit
GCTACGATCTTCTGAGATTAAGCCT298810.1731161975778332No Hit
TCTCAGCTCACCAATCTCTGCT297490.17235145282095513No Hit
CGATTCTATAGACACCCCTCCTCCA276550.1602198200868437No Hit
TGACCACTTTGGATTCTTGCGCCAT254900.14767684736986608No Hit
TGGGTGACCGCCTGGGAATACCCCG243710.1411938974990587No Hit
GACTGTTAGTGAGGAATGTGAAATT236770.13717319400456332No Hit
GAGATTGCTGTAGGCTGCTATGAGC235840.1366343965622174No Hit
TCCGATCTGTTTTCAAGGTTTTGAC221240.12817585606947499No Hit
TATCAGCATCTGTTCAGCATCTGAC219050.1269070749955636No Hit
CTATAGACACCCCTCCTCCAAATAC200150.11595732052208198No Hit
TGGATTTACAAACTTTCATAATGTC199620.11565026391515366No Hit
CCACGATTCTATAGACACCCCTCCT198410.1149492478880154No Hit
GTGACCTCTGGCAGCATGAATGTGC194650.11277088403508996No Hit
ACGATTCTATAGACACCCCTCCTCC189740.10992626528033891No Hit
GAGGTCCGGATTAGGGGAGGGTTAC185880.10768996621855907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)