FastQCFastQC Report
Wed 15 Nov 2023
sRNA-POR-79-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-79-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15794990
Total Bases382.3 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCAC5841063.698046025986721No Hit
ACAAATCTTAGAACAAAGGCTTAAT4503342.851119247305633No Hit
TTCCACTTCAGAGCAAAGATTTTCA4396322.7833635855419976No Hit
AGCGAGAATTCTACCACTGAACCAC2935741.8586526487196258No Hit
TCGGACTGTAGAACTCTGAACGTGT2703411.7115617040593252Illumina RNA PCR Primer (100% over 25bp)
AGGCGAGAATTCTACCACTGAACCA2360531.4944802117633502No Hit
AGACAACTACTCACGCTTAAGCACA2139421.354492785370551No Hit
AGCAGACAACTACTCACGCTTAAGC1905121.206154609784495No Hit
AGTCTCATGCTCTACCGACTGAGCT1277640.8088894010062684No Hit
GCTGAGTTCATCAACAGAATATTTA1173960.7432483338071122No Hit
AATGAGGTCAATGAAGGCGGGGAGG871520.5517698966571046No Hit
AAAGAGGTCTAGTATTTGGAGAAGG870980.5514280160987756No Hit
AAGAGGTCTAGTATTTGGAGAAGGG858820.543729372414924No Hit
AAGCCTACAACACGGGGTATTCCCA780950.494428929679601No Hit
AACAGAGTCGAGTTCGACTATCTTG718640.4549797119213117No Hit
AGTAGATAACCCTCCCCAAGTCCGG689740.436682770929263No Hit
TCCGACACTCAGACAGACATGCTCC679000.4298831464913874No Hit
AAGGATGATAACCCTCGACCGTCCG649220.4110290668116916No Hit
AGTGAGGTCTAAGAGCGGGGAGGGG642160.40655929506761324No Hit
ACACTCTTCTTGGTGCAGGAGTCGA587660.3720546831621926No Hit
GTCTCATGCTCTACCGACTGAGCTA562260.3559736346778314No Hit
GCAAATCTTAGAACAAAGGCTTAAT559490.3542199140360329No Hit
AATGAGGTCAAGTTAGTGGAAGAGG540520.34220977664436636No Hit
AGCCGACAGTACCCTAAGCACTTCC523120.3311936253204339No Hit
GCTCTTCTAGGTGCAGGAGTCGACA513220.3249258150844034No Hit
ACAGAGTCGAGTTCGACTATCTTGA511920.32410276929583365No Hit
AAAGAGGTCAAGTATTTGGAGGAGG486030.30771149586039626No Hit
CGTGGATAACCCTCCTCCGTCCGGA480860.3044383060704692No Hit
ATGAGGTCAAGTTAGTGGAAGAGGG480540.30423571018405204No Hit
GTGGATAACCCTCCTCCGTCCGGAC464980.2943844852070182No Hit
AGCCTACTTCGTCAGGGGCTCAAGT450420.2851663723750379No Hit
AAACTAACCAGCCTAGACCTGA442210.27996852166414793No Hit
AAGAGGTCAAGTATTTGGAGGAGGG419070.26531830662760786No Hit
AAAGTAAACGTCACAGATCCTGAGC406690.25748037827184445No Hit
ACAAGTTCGGATCTACGGGA394350.24966777440188315No Hit
ACTGAGTTCATCAACAGAATATTTA393870.2493638805722574No Hit
CGCTCTTCTAGGTGCAGGAGTCGAC387580.24538160517987032No Hit
GGGCGAGAATTCTACCACTGAACCA373560.236505372906219No Hit
GCGAGAATTCTACCACTGAACCACC323370.20472947434597932No Hit
AGGCACGACCGATCCGCCTAGAAAG322730.20432428257314506No Hit
AGTCTGATGCTCTACCGACTGAGCT319460.20225400585881978No Hit
TTCCACTTCAGAGAAAAGATTTTCA318180.20144362231315122No Hit
GGCAGACAACTACTCACGCTTAAGC318170.20143729119170067No Hit
ATCATCAGAGGACAATTTCACATTC314700.1992403920483647No Hit
GGACAACTACTCACGCTTAAGCACA304980.19308654199844383No Hit
AGAATTCTACCACTGAACCACCAGT293820.18602101045964575No Hit
AGAATCGTGGATAACCCTCCTCCGT275770.17459333624142845No Hit
TGCGAGAATTCTACCACTGAACCAC252450.15982916101877875No Hit
AGGATGATAACCCTCGACCGTCCGG250290.158461638785463No Hit
AGCACACACAGTCTTTGCGAAGAAG248360.15723973234550956No Hit
AGTAGATAACCCTCCCCAAATCCGG243480.15415014507764804No Hit
AATCGTGGATAACCCTCCTCCGTCC239500.15163035874033473No Hit
AATTCAGAAAAACTGAACAGTCATC235980.14940180398974612No Hit
ATCAGAGGACAATTTCACATTCCTC233200.14764175222649714No Hit
GGTGAGGTCTAAGAGCGGGGAGGGG206790.13092126047563182No Hit
AGAGGTCTAGTATTTGGAGAAGGGG197820.12524224453450114No Hit
AAACACAGCAGACAACTACTCACGC196670.12451416556768952No Hit
ACTCTTCTTGGTGCAGGAGTCGACA196170.1241976094951627No Hit
AGTAGTAGATAACCCTCCCCAAGTC186740.11822736196730735No Hit
AACCGGTGTTCTCCCTGTGGTATGG177520.11239006798991326No Hit
TCTCATGCTCTACCGACTGAGCTAG176680.11185825378806824No Hit
GCTTTAAGAAAAGACACACCTCACA170000.10762906465911026No Hit
ACTCTTCTAGGTGCAGGAGTCGACA168960.10697062802825454No Hit
ACACCAAGAAGATGGAATCACACAA168270.10653378064816754No Hit
GAGAATTCTACCACTGAACCACCAG164840.10436220599063374No Hit
AGCCTTCATAAATTCCATCATCAAC163480.10350117347336085No Hit
ACGAGAATTCTACCACTGAACCACC159750.10113966517231096No Hit
AAATGAGGTCAATGAAGGCGGGGAG159620.10105736059345401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)