FastQCFastQC Report
Wed 15 Nov 2023
sRNA-POR-79-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-79-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15794990
Total Bases382.2 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATT11572357.3265953318109105No Hit
GTGTGCTTAAGCGTGAGTAGTTGTC5169113.2726263201179613No Hit
TGAAAATCTTTGCTCTGAAGTGGAA4454382.820122076683809No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC2557611.6192539533105117No Hit
GGAAGAGCACACGTCTGAACTCCAG2452301.5525809133149182Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTCCGGACGGAGGAGGGTTATC2168301.3727770641196986No Hit
TTGCTACGATCTTCTGAGATTAAGC1828301.157518934801478No Hit
TTGTTTAAATATTCTGTTGATGAAC1604181.0156258408520678No Hit
GTTGCTACGATCTTCTGAGATTAAG1549270.980861652967175No Hit
GAGGTCCGGACTTGGGGAGGGTTAT1544930.9781139462576425No Hit
GCACTGTGGTTCAGTGGTAGAATTC1425710.9026343163243534No Hit
AGCTGTCGACTCCTGCACCTAGAAG1379460.8733528796156249No Hit
GAGGTCCGGACGGTCGAGGGTTATC1302680.8247425291184104No Hit
GTTCAAGATAGTCGAACTCGACTCT1212060.767369906533654No Hit
AACTTTTGACGGTGGATCTCTTGGC1142390.7232609833877703No Hit
CTTCGGTACGCCCCTCCCCGCCTTC1125910.7128272952372873No Hit
AGCTGTCGACTCCTGCACCAAGAAG1067490.6758408837232566No Hit
CACCCCTTCTCCAAATACTAGACCT700710.4436280111605009No Hit
CACCCCTCTTCCACTAACTTGACCT638690.40436239592427725No Hit
GCTACGATCTTCTGAGATTAAGCCT612940.38805975818914734No Hit
GCCCGGTTAGCTCAGTCGGTAGAGC574580.3637735763048916No Hit
CGACTTGAGCCCCTGACGAAGTAGG536230.33949372554208646No Hit
GATTCTATAGACACCCCTTCTCCAA527060.33368808717194504No Hit
GGGGACTAGTTGGGAAGTGCTTAGG493690.3125611348915067No Hit
GGTGACCGCCTGGGAATACCCCGTG467550.2960115834198059No Hit
TCAGGTCTAGGCTGGTTAGTTT441800.27970894568467597No Hit
TGCTACGATCTTCTGAGATTAAGCC437250.27682828542468213No Hit
GAGGTCCGGATTTGGGGAGGGTTAT431380.2731119171332176No Hit
GCCTACGACCATACCACAGGGAGAA401720.25433381091092805No Hit
TCCCGTAGATCCGAACTTGT394550.24979439683089386No Hit
GAGGTCCGGAACTCAGGGGAGGGTT379250.24010778101157393No Hit
TGACTGTTAGTGAGGAATGTGAAAT367060.23239014396337068No Hit
GTGTGCTCAGGATCTGTGACGTTTA336560.21308022353923617No Hit
GATTCTATAGACACCCCTCCTCCAA335480.21239646242257829No Hit
TGAAAATCTTTTCTCTGAAGTGGAA322710.20431162033024397No Hit
GTTCTTCTTCGCAAAGACTGTGTGT289930.1835582042153873No Hit
TTGTGTGATTCCATCTTCTTGGTGT277720.17582790492428296No Hit
CACCCCTCCTCCAAATACTTGACCT272800.17271299317061928No Hit
GACTGTTAGTGAGGAATGTGAAATT238980.1513011404249069No Hit
TATTAATGCTGTAGGTGAAAGACCA222470.1408484589100721No Hit
GCGCGGATGAGCCTCACTGGTGTGG219550.13899977144651562No Hit
GTTCAAGATAGTCGAACTCGACCCT211250.13374494064257086No Hit
TCCGATCTGTTTTCAAGGTTTTGAC206060.1304590886097427No Hit
TTGACCTCAGATCAGGCAAGGCTAC204020.1291675398338334No Hit
GTGACCTCTGGCAGCATGAATGTGC197480.1250269864051829No Hit
GACACCCCTTCTCCAAATACTAGAC194680.12325427239903285No Hit
GAGATTGCTGTAGGCTGCTATGAGC193780.12268447146848462No Hit
AGCACTGATGACTGTTCAGTTTTTC192740.12202603483762889No Hit
AGTGTGCTCAGGATCTGTGACGTTT192460.1218487634370139No Hit
TTGTATCTACTTTCAAATCAGCGGC187950.1189934276628222No Hit
TGGGAATACCCCGTGTTGTAGGCTT176240.11157968444424465No Hit
GAAACTCATGAATCACCCCTCTTCC169090.10705293260711149No Hit
GAGTGTGCTCAGGATCTGTGACGTT168850.10690098569229863No Hit
GAGGTCCGGACGGTTGAGGGTTATC165090.10452048402689715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)