FastQCFastQC Report
Wed 15 Nov 2023
sRNA-POR-73-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-73-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14761972
Total Bases352.3 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA11884858.050990748390527No Hit
TCGGACTGTAGAACTCTGAACGTGT4346032.9440714289391687Illumina RNA PCR Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAAT4112432.7858269884267495No Hit
CCGCTTCAAGTCTCAGCCCGAG2920871.9786448585595473No Hit
ACCCGCTTCAAGTCTCAGCCCGAG2877301.9491298317054115No Hit
ACCCGCTTCAAGTCTCAGCCCGAGA2266191.535153975363183No Hit
GGCGAGAATTCTACCACTGAACCAC1953201.323129457229698No Hit
CAGCCCAGGCCGGCTCCAC1003910.6800649669298926No Hit
AGTAGATAACCCTCCCCAAGTCCGG794830.5384307733411228No Hit
CGCTTCAAGTCTCAGCCCGAG767420.5198627933991475No Hit
ACCCGCTTCAAGTCTCAGCCCG752440.5097150976847808No Hit
TGTCTTTCACCTACAGCATTAATA646750.4381189721806815No Hit
AAGAGGTCAAGTATTTGGAGGAGGG615670.41706487453031343No Hit
AATGAGGTCAAGTTAGTGGAAGAGG607180.4113136104038132No Hit
AGCCCAGGCCGGCTCCAC594290.4025817146923189No Hit
ATGAGGTCAAGTTAGTGGAAGAGGG584170.3957262620468322No Hit
AAAGAGGTCAAGTATTTGGAGGAGG584150.3957127137214459No Hit
CACCCGCTTCAAGTCTCAGCCCGAG568480.3850976007812506No Hit
CCGCTTCAAGTCTCAGCCCGAGA564190.3821914849858813No Hit
ACAAGTTCGGATCTACGGGA549390.3721657241999917No Hit
GTGGATAACCCTCCTCCGTCCGGAC527170.357113534695771No Hit
AGTGAGGTCTAAGAGCGGGGAGGGG516520.3498990514275464No Hit
AATTCAGAAAAACTGAACAGTCATC469570.31809435758311966No Hit
CGGGAACAAGCCGAAACATTG469200.3178437135634724No Hit
TCCGACACTCAGACAGACATGCTCC453590.3072692455994362No Hit
GCAAATCTTAGAACAAAGGCTTAAT386960.2621329995748536No Hit
AGCGAGAATTCTACCACTGAACCAC384360.2603717172746297No Hit
AGGCGAGAATTCTACCACTGAACCA369230.25012240911986555No Hit
CGCTCTTCTAGGTGCAGGAGTCGAC352070.2384979459383882No Hit
AAACTAACCAGCCTAGACCTGA349230.2365740837335283No Hit
TCCACTTCAGAGCAAAGATTTTCA340060.23036217654389263No Hit
AATGAGGTCAATGAAGGCGGGGAGG325070.22020770666683287No Hit
GTGAGGTCTAAGAGCGGGGAGGGGG323700.21927964637786876No Hit
ACAGAGTCGAGTTCGACTATCTTGA323120.218886744941665No Hit
TTTTTTTGGTCTTTCACCTACAGCA316730.21455805498073025No Hit
GTCTCATGCTCTACCGACTGAGCTA312460.2116654875107472No Hit
TAGTAGATAACCCTCCCCAAGTCCG302190.20470842242486303No Hit
GCCCGCTTCAAGTCTCAGCCCGAG299440.20284552768424163No Hit
AAGGATGATAACCCTCGACCGTCCG285210.19320589417186268No Hit
GGTGAGGTCTAAGAGCGGGGAGGGG284870.19297557264029494No Hit
ATCATCAGAGGACAATTTCACATTC276700.18744108171997617No Hit
ACACTCTTCTTGGTGCAGGAGTCGA266130.18028079175329692No Hit
AAGCCTACAACACGGGGTATTCCCA262030.1775033850490978No Hit
GCTTTAAGAAAAGACACACCTCACA255240.17290372858043626No Hit
CCGCTTCAAGTCTCAGCCCG253790.17192147498992683No Hit
TCGCTTCAAGTCTCAGCCCGAG252690.17117631709367828No Hit
AGTAGTAGATAACCCTCCCCAAGTC247410.16759955919168523No Hit
CCACTTCAGAGCAAAGATTTTCA237630.1609744280777663No Hit
GCCCGCTTCAAGTCTCAGCCCGAGA236360.16011410941573387No Hit
ACACATCCACTAGCGGTAGGTTGCC227420.15405800796804114No Hit
AGTCTCATGCTCTACCGACTGAGCT225570.15280478786980492No Hit
TTTTTTTTTCCTTCACGAGTAAACA210830.14281967206007437No Hit
GTAGTAGATAACCCTCCCCAAGTCC210410.14253515722696128No Hit
AATGAGGTCAAGTTAGTG201400.13643163664041633No Hit
GCTATTCAACCTCCTGACGATTTTA187340.12690716389382123No Hit
GCTGAGTTCATCAACAGAATATTTA183700.12444136867350786No Hit
GCTCTTCTAGGTGCAGGAGTCGACA180200.12207041173089882No Hit
ACGGGATCGGTCACGGTAA178770.1211017064657757No Hit
TTTTTTGAGCCAAGACGCTATCACT172340.11674591985406828No Hit
CGCTTCAAGTCTCAGCCCGAGA171960.11648850167172786No Hit
TCATTCCGAAGAACAATGGAAGATC171670.11629205095362598No Hit
ACACGTCAGATGCTGAACAGATGCT166490.11278303467856463No Hit
AGAATCGTGGATAACCCTCCTCCGT166450.11275593802779196No Hit
ATCAGAGGACAATTTCACATTCCTC166230.11260690644854224No Hit
GATCTCATTGTGGAAGAAACAAACC152560.10334662604698072No Hit
GTCAAGTAGCTTCTGCTCAGCTCAA151020.10230340499223275No Hit
TTCCACCTCAGAGCAAAGATTTTCA148550.10063018680702009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)