FastQCFastQC Report
Wed 15 Nov 2023
sRNA-POR-73-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-73-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14761972
Total Bases352.3 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA11975188.112181759997918No Hit
GGAAGAGCACACGTCTGAACTCCAG3859552.614521962241901Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGG2924191.9808938805736795No Hit
CTCGGGCTGAGACTTGAAGCGGGT2875091.9476327417502215No Hit
GCACTGGTGGTTCAGTGGTAGAATT2866971.9421321216433685No Hit
TCTCGGGCTGAGACTTGAAGCGGGT2376281.6097307324522767No Hit
GAGGTCCGGACTTGGGGAGGGTTAT2260131.531048832771123No Hit
TTGCTACGATCTTCTGAGATTAAGC1867381.264993592996925No Hit
GAGGTCCGGACGGAGGAGGGTTATC1373350.9303296334663147No Hit
GTTGCTACGATCTTCTGAGATTAAG1112720.7537746311942605No Hit
GTGGAGCCGGCCTGGGCTG1005850.6813791544923673No Hit
CACCCCTCTTCCACTAACTTGACCT805710.5458010623512902No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC794440.5381665809960892No Hit
AGCTGTCGACTCCTGCACCTAGAAG773440.523940839340435No Hit
CTCGGGCTGAGACTTGAAGCG769520.5212853675647129No Hit
CGGGCTGAGACTTGAAGCGGGT752530.5097760651490194No Hit
TATTAATGCTGTAGGTGAAAGACCA701830.4754310602946544No Hit
TATTAATGCTGTAGGTGAAAGACA649810.44019186596479115No Hit
GAGGTCCGGACGGTCGAGGGTTATC623950.42267388124025707No Hit
GTGGAGCCGGCCTGGGCT595740.4035639682828283No Hit
TCTCGGGCTGAGACTTGAAGCGG564770.3825843864220851No Hit
TCCCGTAGATCCGAACTTGT549510.37224701415230976No Hit
AACTTTTGACGGTGGATCTCTTGGC503350.3409774791606433No Hit
GATTCTATAGACACCCCTCCTCCAA490030.3319542944533427No Hit
TAGAATGTTTACTCGTGAAGGAAAA489990.33192719780257No Hit
CAATGTTTCGGCTTGTTCCCG471980.3197269307921733No Hit
CACCCCTCCTCCAAATACTTGACCT458480.3105818111563956No Hit
CTCGGGCTGAGACTTGAAGCGGGTG447980.3034689403285686No Hit
CTTCGGTACGCCCCTCCCCGCCTTC444450.30107766089788No Hit
AGCTGTCGACTCCTGCACCAAGAAG439480.2977109020393752No Hit
AGCACTGATGACTGTTCAGTTTTTC413920.2803961421956362No Hit
GCTACGATCTTCTGAGATTAAGCCT410820.27829615176075395No Hit
ACAGCCCGTTCAAGATAGTCGAACT386830.26204493545984237No Hit
TGACTGTTAGTGAGGAATGTGAAAT360160.24397824355716163No Hit
TCAGGTCTAGGCTGGTTAGTTT348660.2361879564600177No Hit
TGAAAATCTTTGCTCTGAAGTGGA341330.23122249520592508No Hit
CCCCCCTCCCCGCTCTTAGACCTCA341220.23114797941630022No Hit
CTCGGGCTGAGACTTGAAGCGGGC302200.2047151965875562No Hit
TCCCCCCTCCCCGCTCTTAGACCTC273650.18537496209855975No Hit
GCACTGTGGTTCAGTGGTAGAATTC265870.18010466352327453No Hit
GAGGTCCGGATTTGGGGAGGGTTAT265650.17995563194402484No Hit
CGGGCTGAGACTTGAAGCGG254210.17220598982303992No Hit
CTCGGGCTGAGACTTGAAGCGA253140.17148115441487086No Hit
TAGTGATAGCGTCTTGGCTCAAAAA243820.1651676347848377No Hit
TATCAGCATCTGTTCAGCATCTGAC243500.1649508615786563No Hit
TGAAAATCTTTGCTCTGAAGTGG238370.16147571611706077No Hit
CTGGCAACCTACCGCTAGTGGATGT236220.16001927113802952No Hit
TCTCGGGCTGAGACTTGAAGCGGGC235030.15921314577754248No Hit
TGCTACGATCTTCTGAGATTAAGCC224360.1519851141839315No Hit
TTTGCTCTTTCAACACTTCTAACTT222500.15072511992300217No Hit
TGCCTAAAATCGTCAGGAGGTTGAA216360.1465657840293966No Hit
GACTGTTAGTGAGGAATGTGAAATT215260.14582062613314806No Hit
TTGTTTAAATATTCTGTTGATGAAC209220.1417290318664742No Hit
GCCCGGTTAGCTCAGTCGGTAGAGC205330.13909388257883162No Hit
CACTAACTTGACCTCATT202990.13750872850863013No Hit
TATAGACTTGGAAGCGGGAAAAAAA200940.13612002515653057No Hit
TGACGATCTTCCATTGTTCTTCGGA182430.12358105001147543No Hit
GAGGTCCGGACGGTTGAGGGTTATC182040.12331685766644185No Hit
TTACCGTGACCGATCCCGT179710.12173847775893355No Hit
GAGGTCCGGAACTCAGGGGAGGGTT177600.12030912943067498No Hit
TCTCGGGCTGAGACTTGAAGCG172170.11663075908828441No Hit
TAACGGTTTGTTTCTTCCACAATGA171440.11613624521168309No Hit
CTTGATGAAGAAATGCTATGTAAGC170850.11573656961278615No Hit
TTGTAGTTGATTAAGTGGTAATAGC162810.11029014280747856No Hit
TGTATCGTATCTGCAAAGTGCTTGA155770.10552113227148784No Hit
TAGAACTTGCCGATTTGTTATAAAG152090.10302824040040179No Hit
TGAAAATCTTTGCTCTGAGGTGGAA149790.101470182980973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)