FastQCFastQC Report
Wed 14 Feb 2024
sRNA-ACR-178-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-178-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14295261
Total Bases396.9 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5569373.895955449851528No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT3958452.769064517255054No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC2207381.544134101503988No Hit
TGAAAATCTTTGCTCTGAGGTGGAA2091691.463205183871774No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT1647571.1525287995791051No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1623261.135523163935237No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT1476531.032880756776669No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1436031.004549689578945No Hit
TCCCGTAGATCCGAACTTGT1303090.911553835918071No Hit
TCCAAGACCTGAGTTACCACTGACAAGT877130.613580962250357No Hit
TCCCGTAGATCCGAACTTGTGG654470.4578230505899822No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA612180.42823982017537143No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC579570.40542806458727826No Hit
TTGACATGATTTCCGGACTGTTGACCTGG473630.33131958905822007No Hit
TCCAAGACCTGAGTTACCACTGACGAGC451890.3161117519994913No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT355540.24871179336984472No Hit
TTAACGAGTAGATAAATGAAGAGT303690.21244103203152429No Hit
TCCCGTAGATCCGAACTTGC292540.20464124439560777No Hit
TCCAAGACCTGAGTTACCACTGACGAGT283070.19801667139900417No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT280460.19619089151292868No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC279840.19575718134842027No Hit
TACTTCCTTCTTTGAGTTCTTTGTGCTT272410.1905596546995539No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG262350.18352235751414403No Hit
TCACATCGAAAATCGTACATGGATTCACT252680.17675787801286036No Hit
TTCTGGTGGAAATTCTGAGCAGACTGCC243350.17023123956953287No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC242850.16988147330783257No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT232470.16262032571493448No Hit
TCCCGTAGATCCGAACTTGTGA228420.1597872189951621No Hit
TTAAATGACGGGCTAAGAGTGGTATGATT228130.15958435456337594No Hit
TTCTGGTGGAAATTCTGAGCAGACTGCT224890.15731786918755802No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT212130.14839183418896654No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC206470.1444324801065192No Hit
TAAGATTGTGGATTCCGTAAATAAAAAGT199270.139395845938035No Hit
TGATCTAACTCGTATATATAAGCTTACAAT198140.13860537418659233No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT195020.1364228327135825No Hit
GGGGGTGTAGCTCAGTGGTAGAGCATTC181240.1267832745411224No Hit
TTGACATGATTTCCGGACTGTTGACCTGA173180.12114504240251368No Hit
CACCCCTCCCCGCACATTGACCTCTT160850.11251980638898444No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTCGCC155900.10905712039815153No Hit
TCTCAGATTACAGTAGTTAAGT154770.10826664864670886No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCT150380.10519570086898028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)