FastQCFastQC Report
Wed 14 Feb 2024
sRNA-ACR-173-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-173-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16332044
Total Bases453.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2888241.7684498033436598No Hit
TCCAAGACCTGAGTTACCACTGACAAGC2330911.4272004165553314No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT2272981.3917302696465916No Hit
TGAAAATCTTTCTTCTGAGGCGGAA2270551.3902423970937134No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1865871.1424595721147945No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1375010.8419093164333871No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1259000.7708771786311621No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT1252740.7670442230011136No Hit
GCACTGTGGTTCAGTGGTAGAATTCT1045580.6402015571351632No Hit
TCCCGTAGATCCGAACTTGT984380.6027292113589701No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT964800.5907405098835149No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC916890.5614055411557793No Hit
TGAAAATCTTTCTTCTGGGGCGGAA832180.509538181503797No Hit
TCCAAGACCTGAGTTACCACTGACAAGT762740.46702053949891387No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA677240.4146694681939383No Hit
TCCCGTAGATCCGAACTTGTGG661510.40503809566028603No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC629750.38559166262349037No Hit
TCCAAGACCTGAGTTACCACTGACGAGC626320.38349149683897493No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT622830.38135459346056133No Hit
TGAAAATCTTTCTTCTGAGACGGAA542710.3322976597417935No Hit
TCACATCGAAAATCGTACATGGATTCACT541660.33165475184857446No Hit
TAGTAACAATGACCTGATCTGGAGACTCC529170.3240072093854266No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG506340.31002855490715064No Hit
TTGACATGATTTCCGGACTGTTGACCTGG443420.27150306477254166No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC418140.2560242918767547No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC400850.2454377419017485No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT396850.2429885689751999No Hit
CACCCCTCCCCGCACATTGACCTCTT375760.2300753047199726No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC353240.21628646114350414No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT353150.21623135475265678No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC343760.21048192130758403No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT324660.1987871205833146No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA310310.19000071270932162No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA293060.17943865446358093No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGT292220.17892432814900572No Hit
CACAACTTTCAGCGACGGATATCTCGGCT288120.17641392589929344No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC277070.16964808568970302No Hit
TACGAACATACTTTCGGCACAATCTGAAAT251640.15407746880917048No Hit
TCCAAGACCTGAGTTACCACTGACGAGT249670.15287125114284533No Hit
TCCAAGACCTGAGTTACCACTGACAAGCA244670.1498097849846596No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGC226890.1389232113261512No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT226710.13881299854445653No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT221170.13542089404118676No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA217760.1333329741213041No Hit
TGATCTAACTCGTATATATAAGCTTACAAT214890.1315756925465055No Hit
TCCCATATGGTCTAGTGGCTAGGATCCG210850.12910202789069147No Hit
TCCCGTAGATCCGAACTTGC209620.12834890721577777No Hit
TCCAAGACCTGAGTTACCACTGACA208140.1274427132329548No Hit
TCCCGTAGATCCGAACTTGTGA207070.12678755947510303No Hit
TAGTAACAATGACCTGATCTGGAGACTCT203650.12469351662290404No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT202070.12372609331691735No Hit
CGGACCTCGGATCAGCCT201960.12365874106143725No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT194300.11896857490709675No Hit
GCACTGTGGTTCAGTGGTAGAATTCC185790.11375795950586466No Hit
TCTAAGGCCTGAGTTACCACTGACAAGC184200.11278441326756161No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT183630.11243540612552844No Hit
TTAACGAGTAGATAAATGAAGAGT177850.10889635124666575No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC170410.10434088960328543No Hit
TGAGACCATAGACATATCCAACTTCGACA170200.10421230802464161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)