FastQCFastQC Report
Wed 14 Feb 2024
sRNA-ACR-150-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-150-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18395038
Total Bases508.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAGGTGGAA3968242.157233923626578No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2722531.4800349963941364No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT2349581.277290103994349No Hit
TCCCGTAGATCCGAACTTGT2036621.1071572670847432No Hit
TGAAAATCTTTCTTCTGAGGCGGAA2018551.09733396582274No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC1826120.9927242335677697No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1584800.861536681794297No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1450510.7885332990342288No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1295040.7040159416903623No Hit
TCCCGTAGATCCGAACTTGTGG1170200.6361498138791559No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1104280.6003140629554558No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT1080850.5875769324314525No Hit
CACCCCTCCCCGCACATTGACCTCTT758810.4125079817720409No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT753820.409795293709097No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA739350.40192904195142193No Hit
TCTGTCTGGATTTGAGTCAAGTGAAATCT726230.3947966837578699No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT715000.3886917765540903No Hit
TTCGCTACCGGCTCTATACTTTGGTCGT707960.3848646575234039No Hit
TCCAAGACCTGAGTTACCACTGACAAGT615780.33475331771535344No Hit
TGAAAATCTTTCTTCTGAGACGGAA604400.3285668667822268No Hit
TAGTAACAATGACCTGATCTGGAGACTCC585760.3184336993487048No Hit
TTAACGAGTAGATAAATGAAGAGT515060.2799994215831465No Hit
TCTGATCTGACCTACTGACAACATTCATGT497990.27071974518345654No Hit
TCCAAGACCTGAGTTACCACTGACGAGC447720.24339172335496126No Hit
AAAACTGCCGATTTTCCATGA418470.22749069613229395No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT403750.21948853815904049No Hit
TCCCGTAGATCCGAACTTGTGA381160.20720805251938051No Hit
TCCCGTAGATCCGAACTTGC381010.20712650879003347No Hit
TTATGGAATTTAAGGGACATACGACGAGT375920.2043594582408582No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA367690.19988542562401884No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT351460.19106239410867212No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC350260.19041004427389605No Hit
ACCCCTCCCCGCACATTGACCTCTT339180.18438668079946344No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG330660.17975499697255315No Hit
GTGCTAACGATTTGAAGTCTCTGGATTTT327550.17806432365075842No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC321060.17453619829434439No Hit
CGGACCTCGGATCAGCCT309760.1683932373502028No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT295030.16038564312832623No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT272190.14796925127308788No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA260580.14165776662162916No Hit
TAGTAACAATGACCTGATCTGGAGACTCT259120.14086407432265158No Hit
AAAACTGCCGATTTTCCATGAA257810.140151925753021No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTT256950.13968440837143148No Hit
TCACATCGAAAATCGTACATGGATTCACT254820.1385264874147039No Hit
CGGACCTCGGATCAGCCTG250640.13625413549023382No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT235730.12814868879314084No Hit
GAGGTCCGGACTTGGGGAGGGTTATC234480.12746915771524908No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCT223530.12151646547291721No Hit
TAAGATTTAGGAAAATTTGCAGGCACTCCT217860.11843411250360016No Hit
TTCGCTACCGGCTCTATACTTTGGTCGTAT217570.11827646129352926No Hit
TCCAAGACCTGAGTTACCACTGACGAGT210320.11433518104175702No Hit
TCTCAGATTACAGTAGTTAAGT209870.11409054985371599No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGT195480.10626778808502596No Hit
GCACTGTGGTTCAGTGGTAGAATTCT193870.10539255205670138No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT193690.10529469958148495No Hit
CACCCCTCCCCGCACATTGACCTCTTA189260.10288644144143655No Hit
TGAAAATCTTTGCTCTGAGGTGGA184010.10003241091429112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)