FastQCFastQC Report
Wed 14 Feb 2024
sRNA-ACR-145-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-145-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17265123
Total Bases481.1 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTC4948182.8659975373474027No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4635052.684631902130092No Hit
TGAAAATCTTTCTTCTGAGGCGGAA3728142.1593474891548703No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1771751.0262017826342738No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC1757661.0180408213715015No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1549640.8975551462911674No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1460320.8458207914302145No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1339560.7758763143477171No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1051870.6092455871875341No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1010680.5853882419488121No Hit
TCCCGTAGATCCGAACTTGT1006410.5829150478684687No Hit
TCCCGTAGATCCGAACTTGTGG991220.5741169640088866No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT752490.4358439844303455No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT630340.3650944160664248No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA609410.3529717106562172No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG463950.2687209352635368No Hit
TAGTAACAATGACCTGATCTGGAGACTCC452780.262251244894114No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC419820.24316073508425048No Hit
TCCAAGACCTGAGTTACCACTGACAAGT388380.22495061286270593No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA386980.2241397295576753No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC383630.2221994016492092No Hit
TCCAAGACCTGAGTTACCACTGACGAGC370890.21482036357343068No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC350440.2029756752963764No Hit
CACCCCTCCCCGCACATTGACCTCTT346080.2004503530035668No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT300780.17421248606221917No Hit
TCCCGTAGATCCGAACTTGTGA289770.16783546807051417No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT284180.1645977268739991No Hit
TAAAAGACGCTGTATTTCAAAGAAAGGGTT274700.15910688849422042No Hit
TTAACGAGTAGATAAATGAAGAGT272510.15783843532420821No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC272070.15758358628548433No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA263010.15233601289721482No Hit
TCACATCGAAAATCGTACATGGATTCACT241820.14006271487321578No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC228820.13253308418364584No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT216760.12554790371316787No Hit
TCCAAGACCTGAGTTACCACTGACAAGCA211370.12242600298880003No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC209790.1215108632588369No Hit
CGGACCTCGGATCAGCCT205540.11904925322570827No Hit
GAGGTCCGGACTTGGGGAGGGTTATC196460.11379009579022402No Hit
TCCCGTAGATCCGAACTTGC196190.11363371115282526No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT173570.10053215375297356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)