FastQCFastQC Report
Wed 14 Feb 2024
sRNA-ACR-140-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-140-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15076001
Total Bases424.9 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA2763831.833264670120412No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2616771.7357189084824285No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT1635091.0845647993788274No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT1452470.9634318809079411No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1449710.961601156699313No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1189740.7891615289757542No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1121480.7438842701058457No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1097490.7279715622199813No Hit
TGAAAATCTTTCTTCTGAGACGGAA873000.57906602685951No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT867240.5752453850328081No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT738930.4901366085077867No Hit
CACCCCTCCCCGCACATTGACCTCTT730510.4845515730597259No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA655380.43471740284442806No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT537200.3563279148097695No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG494440.32796495569348927No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA487880.32361366916863427No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC484270.3212191349682187No Hit
TCCAAGACCTGAGTTACCACTGACAAGT436850.28976517048519695No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT433660.2876492247513117No Hit
TCCAAGACCTGAGTTACCACTGACGAGC415640.2756964529254144No Hit
GCACTGTGGTTCAGTGGTAGAATTCT410520.2723003268572349No Hit
TCACATCGAAAATCGTACATGGATTCACT396550.26303394381573736No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT392890.26060624432168716No Hit
TCCCGTAGATCCGAACTTGT382470.2536945971282437No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT381270.2528986300810142No Hit
TAGTAACAATGACCTGATCTGGAGACTCC379980.2520429655052424No Hit
TCCCGTAGATCCGAACTTGTGG361350.23968557709700336No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGT356150.23623638655900858No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA324250.21507692922015592No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC284340.1886043918410459No Hit
GAGGTCCGGACTTGGGGAGGGTTATC267030.1771225671847594No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC255150.1692424934171867No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA250240.1659856615822724No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC245740.1630007851551615No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT240790.15971742108533954No Hit
TTGACATGATTTCCGGACTGTTGACCTGG236210.15667948018841335No Hit
AGGTTTTGGTCTAAGTGCTCTTGAAGCTT230190.1526863788348117No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC228310.15143936379415204No Hit
CACCCCTCCCCGAACATTGACCTCAT226560.15027857851694226No Hit
TTAACGAGTAGATAAATGAAGAGT226400.15017244957731163No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGC220680.1463783399855174No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT214280.14213318240029302No Hit
TTATGGAATTTAAGGGACATACGACGAGT189170.12547757193701434No Hit
ACCCCTCCCCGCACATTGACCTCTT188470.12501325782613043No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT179720.11920933144008149No Hit
TCTTTTATGTGCGGATCCTGTCAACCAAGT178720.11854602556739019No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA177770.11791588498833346No Hit
CACCCCTCCCCGCACATTGACCTCT173300.11495090773740331No Hit
TCCAAGACCTGAGTTACCACTGACAAGCA163730.10860307053574751No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT163120.10819845395340581No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAG158600.10520031140884112No Hit
TCTAAGGCCTGAGTTACCACTGACAAGCAGT156680.1039267641332738No Hit
CGGACCTCGGATCAGCCT155880.10339611943512075No Hit
AGGTTTTGGTCTAAGTGCTCTTGAAGCTTT155300.10301140202895981No Hit
TTTCAAAGGGTACTGTGCATGGAAATGAGT153340.10171132251848485No Hit
TCCAAGACCTGAGTTACCACTGACGAGT153340.10171132251848485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)