FastQCFastQC Report
Mon 12 Feb 2024
sRNA-POR-82-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-82-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14464188
Total Bases371.9 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5074943.508624196532844No Hit
TGAAAATCTTTGCTCTGAAGTGGAA3781432.614339636625298No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2585491.7875113348913882No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT2548601.7620069650643368No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT1845801.2761172628563733No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT1206690.8342604507076374No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT1103010.7625799664661439No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC860660.5950282172770431No Hit
TCAGGTCTAGGCTGGTTAGTTT768310.5311808723725107No Hit
TCCCGTAGATCCGAACTTGT765540.5292657977067223No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC665750.46027471434967515No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT439830.3040820542432109No Hit
TTACCGTGACCGATCCCGT422260.2919348116880118No Hit
CTGGCAACCTACCGCTAGTGGATGTGT415460.2872335453604447No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT413570.28592686986645915No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC393830.27227937026261No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC383870.26539339781811466No Hit
TGGGTTGAGTGAATTT382520.26446005817955354No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC367190.25386146806167065No Hit
TGAAAATCTTTTCTCTGAAGTGGAA363360.251213548938938No Hit
GAGGTCCGGACGGAGGAGGGTTAT345230.2386791432744099No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC326140.2254810294224605No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT292230.20203692042719576No Hit
TCTCAGCTCACCAATCTCTGCT287890.1990364063298956No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT269950.18663335957746124No Hit
CACCCCTCTTCCACTAACTTGACCTCAT250570.17323475054389503No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC242190.16744113115786383No Hit
GAGGTCCGGACGGTTGAGGGTTATC233920.16172356166830798No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA211010.14588444232057823No Hit
TGACCACTTTGGATTCTTGCGCCATTCTT210270.14537283392610773No Hit
CACCCCTCTTCCACTAACTTGACCTCATT205830.14230318355928448No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT185250.12807493929144173No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG181920.12577270151632433No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG181540.12550998369213676No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT165470.11439978517978333No Hit
TGGATTTACAAACTTTCATAATGTCTGT164970.1140541038321681No Hit
GTGCTTAGGGTACTGTCGGCT164090.11344570466036531No Hit
TCAAGTCTAGGCTGGTTAGTTT161040.11133704843991242No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGT153290.1059789875518764No Hit
TATCAGCATCTGTTCAGCATCTGACGTGT149740.1035246499838083No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACC146050.10097352163840792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)