FastQCFastQC Report
Mon 12 Feb 2024
sRNA-POR-79-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-79-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12801426
Total Bases328.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4875293.808396033379406No Hit
TGAAAATCTTTGCTCTGAAGTGGAA4170073.2575042811636763No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2438561.9049127808105126No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT2003861.5653412362029044No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT1757051.3725424026979496No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC1108380.8658254166371778No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT641730.5012957150242481No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT602190.47040853104958774No Hit
GTTCAAGATAGTCGAACTCGACTCTGTT589670.46062837062058554No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC583530.45583202996291194No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT555430.4338813504058064No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC487390.3807310216846155No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG440870.34439132015448903No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC437340.3416338148578135No Hit
TCAGGTCTAGGCTGGTTAGTTT426290.33300196399994814No Hit
CGACTTGAGCCCCTGACGAAGTAGGCT425090.33206456843167315No Hit
TCCCGTAGATCCGAACTTGT380920.2975605998894186No Hit
GTTCAAGATAGTCGAACTCGACTCTGT373580.29182686366347No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT370380.2893271421480701No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG356600.2785627163723791No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC305690.23879370938831346No Hit
TGAAAATCTTTTCTCTGAAGTGGAA301640.23562999934538545No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT301020.23514567830177668No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC299800.23419265947403045No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC291800.22794335568553067No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT276590.21606186685764536No Hit
CACCCCTTCTCCAAATACTAGACCTCTT267120.2086642534980087No Hit
CACCCCTCTTCCACTAACTTGACCTCAT257010.20076669583529208No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT253770.19823572780094967No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT232870.1819094216534939No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT229740.17946438154624336No Hit
CACCCCTCTTCCACTAACTTGACCTCATT209010.16327087310429322No Hit
TGGGTTGAGTGAATTT205730.1607086585510083No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA203660.15909165119573399No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGT185280.14473387574165567No Hit
TGGGAATACCCCGTGTTGTAGGCTT168960.13198529601311604No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGT160880.12567349918673124No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA160750.1255719480001681No Hit
TTGTGTGATTCCATCTTCTTGGTGT159680.12473610361845625No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT157970.12340031493366442No Hit
CACCCCTTCTCCAAATACTAGACCTCTTT153280.1197366605876564No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT150590.11763533218877335No Hit
TGAAAATCTTTGCTCTGAAGTGGA146510.11444818725663843No Hit
TCAAGTCTAGGCTGGTTAGTTT141480.11051893749961918No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC141110.11022990719940107No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTT141070.11019866068045857No Hit
GAGGTCCGGAACTCAGGGGAGGGTT140030.10938625118795359No Hit
GAGGTCCGGACGGAGGAGGGTTAT134850.10533982698489996No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCGT132060.10316038228866066No Hit
GTTCAAGATAGTCGAACTCGACCCTGT130730.10212143553382255No Hit
CAATGTTTCGGCTTGTTCCCG130630.10204331923646631No Hit
CACCCCTTCTCCAAATACTAGACCTCT128130.10009041180256012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)