FastQCFastQC Report
Mon 12 Feb 2024
sRNA-POR-73-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POR-73-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13037178
Total Bases316.1 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA11154938.556245837864605No Hit
GAGACTTGAAGCGGGT3346992.5672657073486302No Hit
CTCGGGCTGAGACTTGAAGCGGGT2733692.0968418165342224No Hit
CTCGGGCTGAGACTTGAAGCGG2701842.072411682957769No Hit
TCTCGGGCTGAGACTTGAAGCGGGT2116601.6235108548797907No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1661151.2741637799223116No Hit
GTGGAGCCGGCCTGGGCTG931420.7144337524577788No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT736340.5648001430984527No Hit
CGGGCTGAGACTTGAAGCGGGT714090.5477335662671784No Hit
CTCGGGCTGAGACTTGAAGCG710430.5449262102580789No Hit
TATTAATGCTGTAGGTGAAAGACA603040.46255408954299776No Hit
GTGGAGCCGGCCTGGGCT564540.43302315884618586No Hit
TCTCGGGCTGAGACTTGAAGCGG523110.4012448092677725No Hit
TCCCGTAGATCCGAACTTGT520600.39931954599377256No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC491650.37711382018409195No Hit
GGAGCCGGCCTGGGCTG457540.35095018262387767No Hit
CAATGTTTCGGCTTGTTCCCG434740.3334617353540774No Hit
CTCGGGCTGAGACTTGAAGCGGGTG400750.3073901422531778No Hit
CACCCCTCTTCCACTAACTTGACCTCAT368540.28268387529878014No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT368320.2825151271233698No Hit
GAGACTTGAAGCGGGC352230.2701734992035853No Hit
TCAGGTCTAGGCTGGTTAGTTT331610.25435719294466946No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT328360.251864322171562No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG323990.24851236977818358No Hit
TGAAAATCTTTGCTCTGAAGTGGA319500.24506837292549047No Hit
ACTAACTTGACCTCATT294200.22566233275329983No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA292340.2242356436339214No Hit
CTCGGGCTGAGACTTGAAGCGGGC288860.22156635431379396No Hit
GAGACTTGAAGCGGGTG268930.2062793036959379No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTA266250.20422364410457539No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT263410.20204525856745995No Hit
CACCCCTCTTCCACTAACTTGACCTCATT255710.19613907242809753No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT251190.19267206446057575No Hit
CTCGGGCTGAGACTTGAAGCGA240650.18458749278409792No Hit
CGGGCTGAGACTTGAAGCGG235530.18066026252000242No Hit
TCTCGGGCTGAGACTTGAAGCGGGC225050.17262171307318194No Hit
TGAAAATCTTTGCTCTGAAGTGG221270.1697223126047677No Hit
CACCCCTCCTCCAAATACTTGACCTCTT210390.16137694829356475No Hit
TGAGACTTGAAGCGGGT207780.15937498130346922No Hit
CTGGCAACCTACCGCTAGTGGATGTGT201000.1541744693521865No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA198780.15247164685486383No Hit
CACTAACTTGACCTCATT192580.1477160164569357No Hit
TGCCTAAAATCGTCAGGAGGTTGAATAGC175570.13466871434907154No Hit
TTACCGTGACCGATCCCGT169880.13030427290323104No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC169850.13028126178840235No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC168360.1291383764185777No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA162040.12429070156133482No Hit
TCTCGGGCTGAGACTTGAAGCG158950.1219205567339803No Hit
TGAGACTTGAAGCGG158860.12185152338949426No Hit
TATCAGCATCTGTTCAGCATCTGACGTGT154690.11865297842830712No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC151020.11583795204759802No Hit
TAACGGTTTGTTTCTTCCACAATGAGATC143200.10983972144892093No Hit
TGAAAATCTTTGCTCTGAGGTGGAA139280.10683293577797282No Hit
TGACGATCTTCCATTGTTCTTCGGAATGA137350.10535255405732744No Hit
TCGGGCTGAGACTTGAAGCGGGT135860.10420966868750277No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT130890.10039749399755069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)