FastQCFastQC Report
Wed 14 Feb 2024
sRNA-ACR-178-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-178-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14608168
Total Bases401.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5522103.7801454638254435No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT3916042.6807194440808733No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC2187171.497224018781821No Hit
TGAAAATCTTTGCTCTGAGGTGGAA2069091.4163925277967777No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT1628311.1146572246430901No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1606011.099391792317832No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT1458140.9981676004821413No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1421710.9732294973606547No Hit
TCCCGTAGATCCGAACTTGT1285360.879891304645456No Hit
TCCAAGACCTGAGTTACCACTGACAAGT865860.5927231943115661No Hit
TCCCGTAGATCCGAACTTGTGG643540.44053436406262575No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA605710.41463789299246834No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC574000.3929308589550723No Hit
TTGACATGATTTCCGGACTGTTGACCTGG467390.31995113966378264No Hit
TCCAAGACCTGAGTTACCACTGACGAGC446850.3058905127597109No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT351000.2402765357024919No Hit
TTAACGAGTAGATAAATGAAGAGT299870.20527556911996084No Hit
TCCCGTAGATCCGAACTTGC289350.19807411853423373No Hit
TCCAAGACCTGAGTTACCACTGACGAGT279270.1911738693038032No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT278220.19045509334229999No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC277040.1896473260712774No Hit
TACTTCCTTCTTTGAGTTCTTTGTGCTT268860.18404771905690023No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG259180.17742128924037567No Hit
TCACATCGAAAATCGTACATGGATTCACT250420.17142464407583485No Hit
TTCTGGTGGAAATTCTGAGCAGACTGCC241180.16509941561460684No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC240150.16439433062379896No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT229940.15740509008384898No Hit
TCCCGTAGATCCGAACTTGTGA225790.1545642136645745No Hit
TTAAATGACGGGCTAAGAGTGGTATGATT225150.15412610260232495No Hit
TTCTGGTGGAAATTCTGAGCAGACTGCT222500.15231204898519787No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC216660.14831428554217066No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT208980.14305695279517597No Hit
TAAGATTGTGGATTCCGTAAATAAAAAGT196650.1346164693615243No Hit
TGATCTAACTCGTATATATAAGCTTACAAT195250.13365810141285342No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT192640.13187142973711694No Hit
GGGGGTGTAGCTCAGTGGTAGAGCATTC179230.12269163388591915No Hit
TTGACATGATTTCCGGACTGTTGACCTGA171730.11755751987518215No Hit
CACCCCTCCCCGCACATTGACCTCTT158450.10846671533350383No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTCGCC155050.10613925031530305No Hit
TCTCAGATTACAGTAGTTAAGT152700.10453056125860546No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCT148920.10194296779719401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)