FastQCFastQC Report
Wed 14 Feb 2024
sRNA-ACR-173-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-173-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16583674
Total Bases456.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2861211.725317321119554No Hit
TCCAAGACCTGAGTTACCACTGACAAGC2301771.3879734973082565No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT2244011.3531440620455997No Hit
TGAAAATCTTTCTTCTGAGGCGGAA2241581.3516787655136009No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1839311.1091088741855393No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1359280.8196494938335136No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1246660.7517393311035901No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT1234210.7442319476371761No Hit
GCACTGTGGTTCAGTGGTAGAATTCT1030250.6212435193793606No Hit
TCCCGTAGATCCGAACTTGT969100.5843699050041625No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT951590.5738113279361376No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC906020.5463324954409982No Hit
TGAAAATCTTTCTTCTGGGGCGGAA821890.4956018792940576No Hit
TCCAAGACCTGAGTTACCACTGACAAGT751800.45333742088755485No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA671070.40465701388003644No Hit
TCCCGTAGATCCGAACTTGTGG650930.39251253974239964No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC623020.37568273471849484No Hit
TCCAAGACCTGAGTTACCACTGACGAGC617740.3724988805255096No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT615840.37135317541818536No Hit
TGAAAATCTTTCTTCTGAGACGGAA535560.32294411962029645No Hit
TCACATCGAAAATCGTACATGGATTCACT535210.32273306867947354No Hit
TAGTAACAATGACCTGATCTGGAGACTCC524310.3161603393795609No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG499730.3013385333069138No Hit
TTGACATGATTTCCGGACTGTTGACCTGG436420.2631624331254944No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC411970.24841901740229577No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC397130.2394704575114055No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT392540.23670267517318538No Hit
CACCCCTCCCCGCACATTGACCTCTT370430.22337028574005977No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT349900.21099064055407746No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC349160.21054441856490907No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC338340.2040199294800416No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT320070.1930030703690871No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA307040.18514594534359516No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA288690.17408084601759538No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGT288320.1738577350230112No Hit
CACAACTTTCAGCGACGGATATCTCGGCT284890.17178943580294692No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC273470.16490314510524023No Hit
TACGAACATACTTTCGGCACAATCTGAAAT248590.14990043822617352No Hit
TCCAAGACCTGAGTTACCACTGACGAGT246600.1487004628769234No Hit
TCCAAGACCTGAGTTACCACTGACAAGCA246180.14844720174793594No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGC224140.1351570225029749No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT223310.13465653027188065No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT218660.1318525677723766No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA215340.12985059884799954No Hit
TGATCTAACTCGTATATATAAGCTTACAAT211320.12742652804197668No Hit
TCCCATATGGTCTAGTGGCTAGGATCCG208320.12561751997778056No Hit
TCCCGTAGATCCGAACTTGC207340.12502657734347647No Hit
TCCAAGACCTGAGTTACCACTGACA205610.12398338269312337No Hit
TCCCGTAGATCCGAACTTGTGA204520.1233261097631321No Hit
TAGTAACAATGACCTGATCTGGAGACTCT201080.12125178051618718No Hit
CGGACCTCGGATCAGCCT199710.12042566683353761No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT199490.12029300624216321No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT191680.11558355524837259No Hit
GCACTGTGGTTCAGTGGTAGAATTCC183990.1109464645771498No Hit
TCTAAGGCCTGAGTTACCACTGACAAGC180840.10904700610974384No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT180730.10898067581405664No Hit
TTAACGAGTAGATAAATGAAGAGT175480.10581491170171338No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC168670.10170846339598813No Hit
TGAGACCATAGACATATCCAACTTCGACA168480.10159389288525571No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC165920.10005020600380832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)