FastQCFastQC Report
Wed 14 Feb 2024
sRNA-ACR-150-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-150-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18647411
Total Bases510.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAGGTGGAA3912342.0980606905698598No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2690791.442983157286553No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT2313131.2404563829262947No Hit
TCCCGTAGATCCGAACTTGT2001071.0731087548829166No Hit
TGAAAATCTTTCTTCTGAGGCGGAA1988961.0666145557686266No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC1800010.9652868164915762No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1560980.8371028020994443No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1430830.7673075903137438No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1280460.6866690501968342No Hit
TCCCGTAGATCCGAACTTGTGG1146370.6147609445622237No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1085760.5822577729423135No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT1068730.5731251378542577No Hit
CACCCCTCCCCGCACATTGACCTCTT746490.4003183069220709No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT742150.39799090608342363No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA730410.39169512593464045No Hit
TCTGTCTGGATTTGAGTCAAGTGAAATCT715340.3836135750962962No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT704840.377982766615698No Hit
TTCGCTACCGGCTCTATACTTTGGTCGT696060.3732743381909692No Hit
TCCAAGACCTGAGTTACCACTGACAAGT606770.3253910154069109No Hit
TGAAAATCTTTCTTCTGAGACGGAA596010.3196207773829836No Hit
TAGTAACAATGACCTGATCTGGAGACTCC577500.30969446643290055No Hit
TTAACGAGTAGATAAATGAAGAGT506610.2716784651767476No Hit
TCTGATCTGACCTACTGACAACATTCATGT489540.26252437938971795No Hit
TCCAAGACCTGAGTTACCACTGACGAGC441050.2365207695588412No Hit
AAAACTGCCGATTTTCCATGA412540.22123178386533124No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT398210.21354707095800055No Hit
TCCCGTAGATCCGAACTTGC375610.2014274260378559No Hit
TCCCGTAGATCCGAACTTGTGA375160.20118610567440168No Hit
TTATGGAATTTAAGGGACATACGACGAGT369830.19832780003615516No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA363580.19497612832151337No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC345380.1852160602884765No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT344640.1848192223574629No Hit
ACCCCTCCCCGCACATTGACCTCTT334120.17917768852737787No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG325500.17455506289854392No Hit
GTGCTAACGATTTGAAGTCTCTGGATTTT321590.17245825707386403No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC316500.16972865562945977No Hit
CGGACCTCGGATCAGCCT305690.16393160423181533No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT290970.15603774700949102No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT267990.14371432044909613No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA256740.13768131136274092No Hit
TAGTAACAATGACCTGATCTGGAGACTCT255830.13719330796108906No Hit
AAAACTGCCGATTTTCCATGAA254450.13645325884649617No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTT253170.13576683647933754No Hit
TCACATCGAAAATCGTACATGGATTCACT251840.13505360073846176No Hit
CGGACCTCGGATCAGCCTG246310.13208804160534673No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT232580.12472508918262165No Hit
GAGGTCCGGACTTGGGGAGGGTTATC230960.1238563358741865No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCT220250.11811291122397634No Hit
TAAGATTTAGGAAAATTTGCAGGCACTCCT215320.11546911257546691No Hit
TTCGCTACCGGCTCTATACTTTGGTCGTAT213000.11422497203499188No Hit
TCCAAGACCTGAGTTACCACTGACGAGT207460.1112540502271334No Hit
TCTCAGATTACAGTAGTTAAGT206190.1105729905347182No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGT192430.10319395008776286No Hit
GCACTGTGGTTCAGTGGTAGAATTCT190960.10240563690047912No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT190740.10228765805612372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)