FastQCFastQC Report
Wed 14 Feb 2024
sRNA-ACR-145-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-145-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17548180
Total Bases484.5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTC4879572.780670132173251No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4592412.617029230381726No Hit
TGAAAATCTTTCTTCTGAGGCGGAA3679172.0966105886764326No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1749960.9972316217408301No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC1733370.9877776498759415No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1533980.8741533310007078No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1437920.8194126114503042No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1324520.7547905252852433No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1039880.592585669852942No Hit
TGAAAATCTTTCTTCTGGGGCGGAA997930.5686800568492003No Hit
TCCCGTAGATCCGAACTTGT991040.5647537237479898No Hit
TCCCGTAGATCCGAACTTGTGG973440.5547241936200791No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT740770.42213494504843235No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT621990.3544470138783623No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA599170.3414428162920599No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG457710.2608304678889777No Hit
TAGTAACAATGACCTGATCTGGAGACTCC448130.2553712122852626No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC430950.24558102321722253No Hit
TCCAAGACCTGAGTTACCACTGACAAGT382580.21801691115545885No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA382300.21785735044887847No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC378380.2156235005567529No Hit
TCCAAGACCTGAGTTACCACTGACGAGC366020.20858003508056108No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC346280.19733100526664304No Hit
CACCCCTCCCCGCACATTGACCTCTT341350.1945215971114953No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT297620.16960163390163538No Hit
TCCCGTAGATCCGAACTTGTGA285740.16283170106529565No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT281820.1605978511731701No Hit
TAAAAGACGCTGTATTTCAAAGAAAGGGTT270560.1541812313299727No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC268730.1531383881405365No Hit
TTAACGAGTAGATAAATGAAGAGT267650.15252293970086925No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA259130.1476677353434943No Hit
TCACATCGAAAATCGTACATGGATTCACT238850.13611098130974267No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC225610.1285660393271553No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT213820.12184739386078783No Hit
TCCAAGACCTGAGTTACCACTGACAAGCA211930.12077035909137016No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC207210.11808062146615775No Hit
CGGACCTCGGATCAGCCT203760.11611460561722071No Hit
GAGGTCCGGACTTGGGGAGGGTTATC194200.11066674720683284No Hit
TCCCGTAGATCCGAACTTGC193920.11050718650025244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)