FastQCFastQC Report
Wed 14 Feb 2024
sRNA-ACR-140-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-140-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15287011
Total Bases427.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA2731181.786601710432471No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2596121.6982521959328738No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT1612481.0548039770495357No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1433050.9374298219580008No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT1431990.9367364228363543No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1180550.7722569179808925No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1107930.7247525366469613No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1085510.7100864910740236No Hit
TGAAAATCTTTCTTCTGAGACGGAA863040.5645577150431828No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT857250.5607701858787175No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT728010.47622782504702854No Hit
CACCCCTCCCCGCACATTGACCTCTT721220.47178614576780253No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA649290.4247331280130563No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT530690.3471509244024224No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG488620.3196308290744345No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA481050.31467891270569504No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC478440.3129715809061693No Hit
TCCAAGACCTGAGTTACCACTGACAAGT431260.28210877849175353No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT428850.28053227671518No Hit
TCCAAGACCTGAGTTACCACTGACGAGC410830.2687444916471899No Hit
GCACTGTGGTTCAGTGGTAGAATTCT405520.2652709545378099No Hit
TCACATCGAAAATCGTACATGGATTCACT392400.2566885050321479No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT388660.25424198360294237No Hit
TCCCGTAGATCCGAACTTGT376890.2465426367522075No Hit
TAGTAACAATGACCTGATCTGGAGACTCC376350.24618939569023665No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT376290.24615014668335097No Hit
TCCCGTAGATCCGAACTTGTGG354960.23219712473550258No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGT351600.22999918034990618No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA321190.21010647536002952No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC281410.18408438379484388No Hit
GAGACTCTGCACGGT274770.179740827032832No Hit
GAGGTCCGGACTTGGGGAGGGTTATC264600.1730881203657144No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC259950.17004632233207656No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA247680.16201990042396122No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC242860.15886689687081404No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT237760.15553073128553382No Hit
TTGACATGATTTCCGGACTGTTGACCTGG233230.1525674312656673No Hit
AGGTTTTGGTCTAAGTGCTCTTGAAGCTT227260.14866215508054517No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC225410.14745197736823765No Hit
CACCCCTCCCCGAACATTGACCTCAT223850.14643150318921075No Hit
TTAACGAGTAGATAAATGAAGAGT223180.145993222612321No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGC218270.14278134554884536No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT210610.13777055566977744No Hit
TTATGGAATTTAAGGGACATACGACGAGT186210.12180929286961328No Hit
ACCCCTCCCCGCACATTGACCTCTT185970.1216522968420707No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT177890.11636676391480322No Hit
TCTTTTATGTGCGGATCCTGTCAACCAAGT176230.11528087472430026No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA176060.11516966920479092No Hit
CACCCCTCCCCGCACATTGACCTCT171640.11227832569754806No Hit
TCCAAGACCTGAGTTACCACTGACAAGCA164640.10769927489422228No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT161390.1055732870212496No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAG156450.10234178545433113No Hit
CGGACCTCGGATCAGCCT154100.10080453268464318No Hit
TCTAAGGCCTGAGTTACCACTGACAAGCAGT153770.10058866314677212No Hit
AGGTTTTGGTCTAAGTGCTCTTGAAGCTTT153390.10034008610316301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)