FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-178-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-178-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16505612
Total Bases404.9 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCAC8048984.876511092106128No Hit
TCGGACTGTAGAACTCTGAACGTGT7246264.390179534088164Illumina RNA PCR Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCAC5901993.5757474488071086No Hit
TGCGAGAATTCTACCACTGAACCAC2158271.307597682533674No Hit
TTCCACCTCAGAGCAAAGATTTTCA2083461.2622737042407153No Hit
GCTTGTCAGTGGTAACTCAGGTCTT1623490.9835987905204605No Hit
ACTTGGTAGACAGGTTCCGCACATA1509910.9147858316310841No Hit
ACAAGTTCGGATCTACGGGA1299470.7872898017958982No Hit
ACTTGTCAGTGGTAACTCAGGTCTT891530.540137499900034No Hit
AGGCGAGAATTCTACCACTGAACCA866240.5248154385308463No Hit
CCACAAGTTCGGATCTACGGGA651910.3949626345269718No Hit
GAGTGGATAACCCTCCCCAAGTCCG595040.36050768671891714No Hit
CCAGGTCAACAGTCCGGAAATCATG485910.2943907805417939No Hit
GCTCGTCAGTGGTAACTCAGGTCTT450620.2730101737518124No Hit
ACTGCTTGTCAGTGGTAACTCAGGT366460.22202145549041138No Hit
AAGAGGTCAATGTGCGGGGAGGGGT339040.20540892394659466No Hit
ACTCTTCATTTATCTACTCGTTAA302720.18340428697827138No Hit
AGTGGATAACCCTCCCCAAGTCCGG296650.17972674990784954No Hit
GCAAGTTCGGATCTACGGGA288160.17458304484559553No Hit
ACTCGTCAGTGGTAACTCAGGTCTT287460.17415894666613999No Hit
GCTTGGTAGACAGGTTCCGCACATA283100.17151742086267385No Hit
AGGGACGCTCGGACAGCCTTGCTCG275670.16701592161502404No Hit
AAGCACAAAGAACTCAAAGAAGGAA274680.16641612561836544No Hit
AGCCGGAGATCCTAACCACCTAGAC270080.16362919472480028No Hit
CTGCTTGTCAGTGGTAACTCAGGTC267640.16215090964212656No Hit
AGTGAATCCATGTACGATTTTCGAT258690.1567285114905161No Hit
GAGAATTCTACCACTGAACCACCAG249970.15144545988358385No Hit
GGCAGTCTGCTCAGAATTTCCACCA249730.1513000547934848No Hit
AGCAGTCTGCTCAGAATTTCCACCA234010.14177602139199685No Hit
AATCATACCACTCTTAGCCCGTCAT231120.14002510176538743No Hit
TCACAAGTTCGGATCTACGGGA227690.13794702068605516No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG222720.1349359236119206No Hit
AGAGTGGATAACCCTCCCCAAGTCC221100.133954439253752No Hit
GGGCGAGAATTCTACCACTGAACCA218170.1321792854454594No Hit
AGTTTTGGCTTAACAAGTCTGGAGG217470.13175518726600383No Hit
TGGCGAGAATTCTACCACTGAACCA217380.13170066035721667No Hit
ACACGCTTTGTCCATAACAAGAGAC216850.1313795574499146No Hit
AATCAGAATCAAGTTTTCTTCAAGA211530.1281564112860523No Hit
GCGAGAATTCTACCACTGAACCACC208930.12658118947664587No Hit
ACTTTTTATTTACGGAATCCACAAT202050.12241291022713971No Hit
ATTGTAAGCTTATATATACGAGTTA200370.12139507459644634No Hit
AAGCCTACGACACGGGGTATTCCCA186000.11268894482676559No Hit
CGCGAGAATTCTACCACTGAACCAC185220.11221637828394368No Hit
GAATGCTCTACCACTGAGCTACACC183780.11134394774334935No Hit
AAGAGTGGATAACCCTCCCCAAGTC177770.10770276194545225No Hit
TCAGGTCAACAGTCCGGAAATCATG177650.10763005940040273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)