FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-178-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-178-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16505612
Total Bases404.9 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATT13056497.910333770114068No Hit
GGAAGAGCACACGTCTGAACTCCAG7046494.269147972216965Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTC5325303.2263571929353483No Hit
TCCAAGACCTGAGTTACCACTGACA3716622.2517311081830838No Hit
GAGGTCCGGACTTGGGGAGGGTTAT3205561.942103085907993No Hit
TCTTTTATGTGCGGAACCTGTCTAC2171001.3153102108543444No Hit
TGAAAATCTTTGCTCTGAGGTGGAA2096151.2699619983797026No Hit
TCCCGTAGATCCGAACTTGT1300670.7880168272463935No Hit
TCCAAGACCTGAGTTACCACTGACG1137630.6892383027057706No Hit
TTGACATGATTTCCGGACTGTTGAC775260.4696947922924639No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC678640.4111571264367538No Hit
TCCCGTAGATCCGAACTTGTGG653100.39568360143204623No Hit
TTCTGGTGGAAATTCTGAGCAGACT598770.36276752416087327No Hit
GAGGTCCGGACCTGGGGAGGGTTAT501960.30411474594216803No Hit
GGGGGTGTAGCTCAGTGGTAGAGCA489200.2963840419852351No Hit
TCCAACGTAGTCTAGGTGGTTAGGA438640.2657520363377014No Hit
TACTTCCTTCTTTGAGTTCTTTGTG421090.25511928912420817No Hit
TACGATATGTCTTAATTCTCTGAAT410030.2484185378888102No Hit
TCACATCGAAAATCGTACATGGATT378820.22950981763051256No Hit
GCACTTGTGGTTCAGTGGTAGAATT372510.22568687546999167No Hit
TCTTTTATGAGTGGAACCTCTCGAC342280.20737189266293185No Hit
CACCCCTCCCCGCACATTGACCTCT337600.20453649340600033No Hit
TGATCTTTCGTCGCGTGGTTGCTCG329130.19940490543458791No Hit
TATGGTCTCTTGTTATGGACAAAGC328990.19932008579869684No Hit
GAGACTCTGCACGGTGGATCTCTCG324190.19641198399671578No Hit
TCTTCTGTTCTGCATATAAATTAGT309710.1876392102274063No Hit
TTAACGAGTAGATAAATGAAGAGT303120.18364662879510316No Hit
TCGGTACTCTGTATTTCTTCCTGAG299170.18125350335388957No Hit
TCCCGTAGATCCGAACTTGC292630.1772912146486904No Hit
TTAAATGACGGGCTAAGAGTGGTAT291210.17643090119893767No Hit
GGCCGCGATCGTCTAGTGGTTAGGA290770.17616432520042274No Hit
TAGAGCCTCCAGACTTGTTAAGCCA276920.16777323979262326No Hit
TCCAAGGCCTGAGTTACTACTGACG266950.16173287000809178No Hit
TAAGATTGTGGATTCCGTAAATAAA265270.16071503437739842No Hit
TCTTTTATGTGCGGAACCTGTCAAC263180.1594487983844525No Hit
CGGACCTCGGATCAGCCTGGATCAC263080.15938821293024458No Hit
TGATCTAACTCGTATATATAAGCTT239230.14493858210165125No Hit
TCCCGTAGATCCGAACTTGTGA227810.13801972323110465No Hit
TCCTCGGTAGTATAGTGGTGAGTAT220370.13351216543803404No Hit
GACCGTGGAATTGAACTACTGCTTC218890.13261550071575656No Hit
AGGTTTTGGTCTAAGTGCTCTTGAA217960.13205205599162273No Hit
TCCAAGGCCTGAGTTACCACTGACG217340.13167642617553352No Hit
TTTTATGATGAATCTTTTAGACTAG216800.13134926472281064No Hit
TCTAAGGCCTGAGTTACCACTGACA212590.12879861710065643No Hit
TGGATTCCAAAGACTGTGTAAGATA201960.12235838331835257No Hit
TCCAAGGCCTGAGTTACCACTGACA199800.12104973750746109No Hit
TTATTCAGGATCTTGTACCAAATGG199020.12057717096463919No Hit
TCCATCGTAGTCTAGGTGGTTAGGA197540.11968050624236168No Hit
TCACGTGTAACTGTGCATTTCCTTG192290.11649976989644491No Hit
TAGTACTTTTGCTGAGTTCCTGAGA191560.11605749608072696No Hit
GGCTGTTTGGTCTAGTGGTATGATT186740.11313727718790434No Hit
TGATAAACTTTCTCGATTTATTCTG173530.10513393868703566No Hit
TGTGATTGTGTCCTCTTCTGGAGTG172190.10432209360064928No Hit
TAAGACCATGCCGATTAAAGAATTT170730.1034375459692134No Hit
GGCCCTGGCCCAATGGATAAGGCAT169650.10278322306376765No Hit
TCCGTAAACTCCTGTCCACTTACAA168040.10180779725101982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)