FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-173-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-173-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18566189
Total Bases457.1 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGT4457162.4006865383089657Illumina RNA PCR Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCAC4306112.319328969450866No Hit
GCTTGTCAGTGGTAACTCAGGTCTT2338691.2596500014084742No Hit
AGAATTCTACCACTGAACCACCAGT2302501.2401575789194001No Hit
TTCCGCCTCAGAAGAAAGATTTTCA2263901.2193670979003823No Hit
AGCGAGAATTCTACCACTGAACCAC2108201.1355049762770377No Hit
AGAATCATACCACTAGACCAAACAG1907381.0273406136283543No Hit
AGGCGAGAATTCTACCACTGAACCA1378620.7425433404776822No Hit
ACTGCTTGTCAGTGGTAACTCAGGT1287680.6935618289784726No Hit
AGAATTCTACCACTGAACCACAGTG1057570.5696214769762389No Hit
TGCGAGAATTCTACCACTGAACCAC1041920.5611921757340723No Hit
ACTTGGTAGACAGGTTCCGCACATA984560.5302973054944125No Hit
ACAAGTTCGGATCTACGGGA982090.5289669301545945No Hit
GAGAATTCTACCACTGAACCACCAG929080.5004150286308083No Hit
TTCCGCCCCAGAAGAAAGATTTTCA829190.44661292632537564No Hit
ACTTGTCAGTGGTAACTCAGGTCTT776960.4184811433299532No Hit
AAGAGGTCAATGTGCGGGGAGGGGT690230.371767194656911No Hit
CCACAAGTTCGGATCTACGGGA658320.35458003793885756No Hit
GAGTGGATAACCCTCCCCAAGTCCG643460.34657624136003357No Hit
GCTCGTCAGTGGTAACTCAGGTCTT626090.33722052490147547No Hit
AGGGACGCTCGGACAGCCTTGCTCG559660.3014404302358443No Hit
AGTGAATCCATGTACGATTTTCGAT555410.2991513228697607No Hit
TTCCGTCTCAGAAGAAAGATTTTCA542250.29206316923737013No Hit
GGAGTCTCCAGATCAGGTCATTGTT540680.29121754604566397No Hit
CTGCTTGTCAGTGGTAACTCAGGTC517050.27849010909023925No Hit
CCAGGTCAACAGTCCGGAAATCATG458490.24694890265309696No Hit
GAGAATTCTACCACTGAACCACAGT421750.22716024274017677No Hit
GGAATTCTACCACTGAACCACCAGT400540.21573625044967493No Hit
AGCCGAGATATCCGTCGCTGAAAGT399280.21505759744231842No Hit
TGGCTCCCCGGCGGGGAATCGAACC393400.21189055007465452No Hit
AGTGGATAACCCTCCCCAAGTCCGG367900.19815590587815304No Hit
GGAATCATACCACTAGACCAAACAG357030.19230117715595807No Hit
TGGCGAGAATTCTACCACTGAACCA354880.19114315813546873No Hit
GCCGGATCCTAGCCACTAGACCATA348560.18773912082872798No Hit
GGGCGAGAATTCTACCACTGAACCA335750.1808394819206031No Hit
ACTTGGTCGAGAGGTTCCACTCATA331280.17843187958498105No Hit
GCTTGTCAGTGGTAACTCAGGCCTT306410.16503656189215782No Hit
TTTTTTTGGCCTTTAACCTACAGCA301570.16242967256231206No Hit
ACTGCTCGTCAGTGGTAACTCAGGT300010.1615894355055849No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG286680.154409717578551No Hit
GAGAATCATACCACTAGACCAAACA281830.15179744211372617No Hit
AGTTTTGGCTTAACAAGTCTGGAGG258260.1391023219681756No Hit
ATTTCAGATTGTGCCGAAAGTATGT256530.1381705206168051No Hit
ACTCGTCAGTGGTAACTCAGGTCTT254020.13681860073707103No Hit
TGCTTGTCAGTGGTAACTCAGGTCT248060.13360846428957499No Hit
ACTGCTCGTCAGTAGTAACTCAGGC233160.1255831231708349No Hit
GCTGCTTGTCAGTGGTAACTCAGGT230480.12413963899645748No Hit
TTTCATTTGTATCCTCACGGTTGAC223220.12022930500168882No Hit
ATTGTAAGCTTATATATACGAGTTA217540.11716998033360534No Hit
CGGATCCTAGCCACTAGACCATATG214670.11562415959462656No Hit
AGAGTCTCCAGATCAGGTCATTGTT209530.11285568621541017No Hit
TGTCAGTGGTAACTCAGGTCTTGGA207570.11180000375952222No Hit
GCAAGTTCGGATCTACGGGA206800.11138527136613766No Hit
ACTTGGTTGACAGGTTCCGCACATA206490.11121830118178803No Hit
TCACAAGTTCGGATCTACGGGA206490.11121830118178803No Hit
AGGCTGATCCGAGGTCCG201270.10840673872273948No Hit
ACTTGGTCGACAGGTTCTGCACATA197980.10663470031464185No Hit
AAGCCTACGACACGGGGTATTCCCA196590.10588602755255805No Hit
ACACGCTTTGTCCATAACAAGAGAC186890.10066147662290846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)