FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-173-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-173-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18566189
Total Bases457.1 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATT10797095.815458412062917No Hit
TCCAAGACCTGAGTTACCACTGACA6070043.2694054768051752No Hit
GCACTGTGGTTCAGTGGTAGAATTC4840712.6072717454292857No Hit
GGAAGAGCACACGTCTGAACTCCAG4302472.3173684163185024Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTCCGGACTTGGGGAGGGTTAT3484011.8765348128256154No Hit
GGCTGTTTGGTCTAGTGGTATGATT2852181.5362226464461823No Hit
TGAAAATCTTTCTTCTGAGGCGGAA2277331.2266006771772064No Hit
TCCAAGACCTGAGTTACCACTGACG1723320.9282034132045085No Hit
TCTTTTATGTGCGGAACCTGTCTAC1445050.7783234351433135No Hit
TCCCATATGGTCTAGTGGCTAGGAT1191880.6419626558794591No Hit
TCCCGTAGATCCGAACTTGT982930.5294193654928322No Hit
TAGTAACAATGACCTGATCTGGAGA941140.5069107074155067No Hit
TCACATCGAAAATCGTACATGGATT876580.4721378199909524No Hit
TGAAAATCTTTCTTCTGGGGCGGAA834240.44933292448978085No Hit
TCTTTTATGAGTGGAACCTCTCGAC802100.4320218866672099No Hit
TTGACATGATTTCCGGACTGTTGAC774220.4170053423457017No Hit
CACCCCTCCCCGCACATTGACCTCT731080.39376955604620856No Hit
TCCAAGGCCTGAGTTACTACTGACG663970.357623204202004No Hit
TCCCGTAGATCCGAACTTGTGG661730.35641670996670344No Hit
CACAACTTTCAGCGACGGATATCTC643680.346694736329572No Hit
CGGACCTCGGATCAGCCTGGATCAC637520.3433768771824956No Hit
GAGACTCTGCACGGTGGATCTCTCG605020.3258719385006799No Hit
GAGGTCCGGACCTGGGGAGGGTTAT575040.30972430583357735No Hit
TATTAATGCTGTAGGTTAAAGGCCA562360.30289468668017977No Hit
TCTAAGGCCTGAGTTACCACTGACA550310.2964043940304604No Hit
TGAAAATCTTTCTTCTGAGACGGAA544080.2930488319385308No Hit
TCTTTTATGTGCGGAACCTGTCAAC518540.2792926432021133No Hit
TCCAAGGCCTGAGTTACCACTGACG457100.2462002298910132No Hit
TCTTTTATGTGCAGAACCTGTCGAC440540.23728079036575575No Hit
TCCAAGGCCTGAGTTACCACTGACA420480.2264762035978412No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC396280.2134417569486123No Hit
GCGGGAGACCGGGGTTCGATTCCCC394110.21227296565816495No Hit
TCATCACTCGAGTCTGGTTGAATGG376180.20261562564078175No Hit
AGTGATTGATAGGCGGGATGGATTC354790.19109468292065757No Hit
TAGAGCCTCCAGACTTGTTAAGCCA340810.1835648662199873No Hit
TGAGACCATAGACATATCCAACTTC335650.1807856205708129No Hit
TTACGTATCTGTTGTCTCAATCTGG319520.1720977848496533No Hit
TACGAACATACTTTCGGCACAATCT311120.16757343146727635No Hit
TTGCAAGTTCCCTGAAATCTCCGAA308070.16593066029867518No Hit
TACGATTTGGCGATACTACATCTGA302150.16274206839109523No Hit
GGCCGCGATCGTCTAGTGGTTAGGA300160.16167022753027022No Hit
TATGGTCTCTTGTTATGGACAAAGC297490.16023212949087182No Hit
GCACTTGTGGTTCAGTGGTAGAATT294030.15836852678813082No Hit
TGCTTACAATGTCGTCTGCGACCGA289880.15613328077183744No Hit
AGGGGTCAACCGTGAGGATACAAAT286180.15414041082959998No Hit
TGATCTTTCGTCGCGTGGTTGCTCG279870.15074175965783823No Hit
TGGATCGATGACATCAAATATTGTA277220.14931443388839788No Hit
AGACTCTGCACGGTGGATCTCTCGG275730.14851189977652388No Hit
TGATCTAACTCGTATATATAAGCTT262250.14125138982480465No Hit
TCAGTCACAAGGTATTGCTCAGCAA261640.14092283559108443No Hit
TTGGATCCACATATCAAACACTTGA253660.1366246998778263No Hit
ATAGCATGAAGTCAAACAAGAGAAC242810.13078074342558937No Hit
TTATTCAGGATCTTGTACCAAATGG240050.1292941701713798No Hit
TCCTCGGTAGTATAGTGGTGAGTAT218880.11789172242079407No Hit
ACAACTTTCAGCGACGGATATCTCG215670.11616277309252857No Hit
TCCCGTAGATCCGAACTTGC210010.11311422069440315No Hit
AAAGGAAAGGTTAACCGCTGAAAAA209760.11297956731992763No Hit
TCCCGTAGATCCGAACTTGTGA206950.11146606339082296No Hit
CCACCCCTCCCCGCACATTGACCTC206520.11123445958672509No Hit
CGGACCTCGGATCAGCCT202730.10919311442967644No Hit
TCCAACGTAGTCTAGGTGGTTAGGA197310.10627382927104749No Hit
TAGTACTTTTGCTGAGTTCCTGAGA193970.10447486018805474No Hit
TGATTATCTTCTCTTAGTAACACTC192720.10380159331567723No Hit
CACCCCTCCCCGAACATTGACCTCA187940.10122702079570557No Hit
TGATTGGTTCTCATGTGACCTCATA187130.10079074386240494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)