FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-150-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-150-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21281386
Total Bases521.8 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGT5718932.6872920776870455Illumina RNA PCR Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCAC4167981.9585096572187544No Hit
TTCCACCTCAGAGCAAAGATTTTCA3955711.8587652138822162No Hit
ACTTGGTAGACAGGTTCCGCACATA2401331.1283710562836462No Hit
AGCGAGAATTCTACCACTGAACCAC2284421.0734357245341068No Hit
ACAAGTTCGGATCTACGGGA2030830.9542752525610879No Hit
TTCCGCCTCAGAAGAAAGATTTTCA2012670.9457419737605437No Hit
GAGTGGATAACCCTCCCCAAGTCCG1867690.8776167116183129No Hit
GCTTGTCAGTGGTAACTCAGGTCTT1591230.7477097591294101No Hit
AAGAGGTCAATGTGCGGGGAGGGGT1473880.6925676739287563No Hit
TGCGAGAATTCTACCACTGAACCAC1167060.5483947333129524No Hit
CCACAAGTTCGGATCTACGGGA1166030.5479107422796616No Hit
AGAATCATACCACTAGACCAAACAG1128770.5304024841239194No Hit
AGTGGATAACCCTCCCCAAGTCCGG1125320.5287813491094988No Hit
ACTGCTTGTCAGTGGTAACTCAGGT774910.36412572000714616No Hit
AGATTTCACTTGACTCAAATCCAGA739080.347289410567526No Hit
ACGACCAAAGTATAGAGCCGGTAGC718360.33755320259686095No Hit
GGAGTCTCCAGATCAGGTCATTGTT660620.31042151108015237No Hit
ACTTGTCAGTGGTAACTCAGGTCTT626840.29454848476504303No Hit
TTCCGTCTCAGAAGAAAGATTTTCA603880.2837597137705223No Hit
AGGGACGCTCGGACAGCCTTGCTCG587980.27628839587797527No Hit
TGGCTCCCCGGCGGGGAATCGAACC577100.27117594690496194No Hit
ACTCTTCATTTATCTACTCGTTAA513150.24112621236229634No Hit
ACATGAATGTTGTCAGTAGGTCAGA511070.2401488324115732No Hit
TAAGAGGTCAATGTGCGGGGAGGGG490460.23046431280368673No Hit
AGAGTGGATAACCCTCCCCAAGTCC483090.22700119249751874No Hit
AATCAGAATCAAGTTTTCTTCAAGA463170.2176408998925164No Hit
AGGCGAGAATTCTACCACTGAACCA451590.2121995249745482No Hit
GCTCGTCAGTGGTAACTCAGGTCTT447240.210155485173757No Hit
TCATGGAAAATCGGCAGTTTT412460.1938125646515692No Hit
AGAATTCTACCACTGAACCACCAGT411080.19316411064580097No Hit
ACTCGTCGTATGTCCCTTAAATTCC381070.1790625854913773No Hit
AAGAGTGGATAACCCTCCCCAAGTC380850.17895920876582003No Hit
TCACAAGTTCGGATCTACGGGA379750.1784423251380338No Hit
GCAAGTTCGGATCTACGGGA375640.17651105994694144No Hit
ACTGCTCGTCAGTAGTAACTCAGGC359770.16905383888060677No Hit
GCTTGGTAGACAGGTTCCGCACATA355330.1669675086011785No Hit
CTGCTTGTCAGTGGTAACTCAGGTC337100.15840133720613875No Hit
AAAATCCAGAGACTTCAAATCGTTA336470.1581053038556793No Hit
GAGAATTCTACCACTGAACCACCAG329440.15480194757991797No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG325910.1531432210289311No Hit
AGGCTGATCCGAGGTCCG308830.14511742797203153No Hit
AGTGGATAACCCTCCCCAGGTCCGG304180.14293241990911684No Hit
AGAGTCTCCAGATCAGGTCATTGTT294350.13831335985353585No Hit
ATGAGGTCAATGTTCGGGGAGGGGT280450.13178183037514568No Hit
TAAGAGTGGATAACCCTCCCCAAGT278610.13091722503412137No Hit
ACTTGGTCGAGAGGTTCCACTCATA278390.1308138483085641No Hit
AAGTGGATAACCCTCCCCAAGTCCG268900.12635455228338982No Hit
TGGATAACCCTCCCCAAGTCCGGAC268720.1262699713261157No Hit
AGTGAATCCATGTACGATTTTCGAT263040.12360097222991022No Hit
TTCATGGAAAATCGGCAGTTTT254190.1194424084972661No Hit
CAGGCTGATCCGAGGTCCG249900.11742656234889964No Hit
TGGATCGTAGAACCAAGGAAGAATC248410.11672641998035278No Hit
AAAGAGGTCAATGTGCGGGGAGGGG246580.11586651358139925No Hit
GGCGAGAATTCTACCATTGAACCAC244530.11490323045688848No Hit
AGAGGTCAATGTGCGGGGAGGGGTG243790.11455550874365043No Hit
ACTTGGTTGACAGGTTCCGCACATA241560.11350764466186553No Hit
AAGCCTACGACACGGGGTATTCCCA240100.11282159911953105No Hit
GATAACCCTCCCCAAGTCCGGACCT236310.11104070007470378No Hit
GCCGACCCTCTGCCAGGCGTGGCCA234490.11018549261782103No Hit
AGGGTGATAACCCTCCCCAAGTCCG231930.10898256344770026No Hit
ATACGACCAAAGTATAGAGCCGGTA230790.10844688405163086No Hit
GAGGGACGCTCGGACAGCCTTGCTC230300.10821663589016242No Hit
GCTTGTCAGTGGTAACTCAGGCCTT226320.10634645694599026No Hit
AGGAGTGCCTGCAAATTTTCCTAAA224980.10571679870850516No Hit
ACTCGTCAGTGGTAACTCAGGTCTT213950.10053386560443009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)