FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-150-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-150-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21281386
Total Bases521.7 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCCGGACTTGGGGAGGGTTAT8377063.9363319663484324No Hit
GCACTGGTGGTTCAGTGGTAGAATT6729303.1620590876928785No Hit
GGAAGAGCACACGTCTGAACTCCAG5515892.5918847578818407Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAGGTGGAA3980041.8701977399404344No Hit
TCCAAGACCTGAGTTACCACTGACA3921991.8429203812195316No Hit
GCACTGTGGTTCAGTGGTAGAATTC3250651.5274616042394984No Hit
TCTTTTATGTGCGGAACCTGTCTAC3099561.4564652884920184No Hit
TCCCGTAGATCCGAACTTGT2033740.9556426447036861No Hit
TGAAAATCTTTCTTCTGAGGCGGAA2023030.950610077745876No Hit
GGCTGTTTGGTCTAGTGGTATGATT1698130.7979414498660943No Hit
CACCCCTCCCCGCACATTGACCTCT1439990.6766429592508684No Hit
GAGGTCCGGACCTGGGGAGGGTTAT1263820.5938616967898613No Hit
TTCGCTACCGGCTCTATACTTTGGT1239520.5824432675578555No Hit
TCCAAGACCTGAGTTACCACTGACG1185650.5571300666225405No Hit
TCCCGTAGATCCGAACTTGTGG1169540.5495600709465069No Hit
TAGTAACAATGACCTGATCTGGAGA1048570.4927169687162293No Hit
TCTGTCTGGATTTGAGTCAAGTGAA1000850.47029361715444656No Hit
GAGACTCTGCACGGTGGATCTCTCG893620.41990686132942656No Hit
TCTGATCTGACCTACTGACAACATT698470.32820700681807097No Hit
TCCAAGGCCTGAGTTACTACTGACG678160.31866345547230807No Hit
TCTTTTATGAGTGGAACCTCTCGAC640460.3009484438654513No Hit
TGAAAATCTTTCTTCTGAGACGGAA606300.2848968577516521No Hit
TCTTTTATGTGCGGAACCTGTCAAC570830.2682297102265802No Hit
ACCCCTCCCCGCACATTGACCTCTT546210.2566609148483092No Hit
TTATGGAATTTAAGGGACATACGAC543730.2554955772147547No Hit
TTAACGAGTAGATAAATGAAGAGT514170.2416055044535163No Hit
TTTTATGATGAATCTTTTAGACTAG485990.22836388569804617No Hit
CGGACCTCGGATCAGCCTGGATCAC485620.22819002484142717No Hit
TCTAAGGCCTGAGTTACCACTGACA474230.22283792982280382No Hit
GTGCTAACGATTTGAAGTCTCTGGA439300.20642452516955426No Hit
TCACATCGAAAATCGTACATGGATT437050.20536726320362783No Hit
AAAACTGCCGATTTTCCATGA418830.19680579075065882No Hit
GCACTGGTGGTTCAATGGTAGAATT410740.1930043466153943No Hit
TCTTTTATGTGCAGAACCTGTCGAC410260.19277879739599668No Hit
TCCCGTAGATCCGAACTTGC381890.1794478987411816No Hit
TCCCGTAGATCCGAACTTGTGA380300.1787007669519269No Hit
TCCCATATGGTCTAGTGGCTAGGAT377520.17739446105624887No Hit
CACCCCTCCCCGAACATTGACCTCA367290.17258744331783654No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC354950.1667889488024887No Hit
TAAGATTTAGGAAAATTTGCAGGCA350470.16468382275477733No Hit
GACCGTGGAATTGAACTACTGCTTC347610.16333992532253302No Hit
CCACCCCTCCCCGCACATTGACCTC330300.1552060565980054No Hit
TGCTTACAATGTCGTCTGCGACCGA323560.1520389696422968No Hit
CGGACCTCGGATCAGCCT310800.146043119559976No Hit
TGACTGTAGTCTTTTACAATTGAAC293760.13803612227135958No Hit
AGGTTTTGGTCTAAGTGCTCTTGAA281740.13238799390227687No Hit
TCTTCTGTTCTGCATATAAATTAGT278500.13086553667134274No Hit
TATTAATGCTGTAGGTTAAAGGCCA273670.12859594765115392No Hit
GCGGGAGACCGGGGTTCGATTCCCC264120.12410845797355492No Hit
TGATTGGTTCTCATGTGACCTCATA264040.12407086643698864No Hit
AAAACTGCCGATTTTCCATGAA257820.12114812446896081No Hit
CACAACTTTCAGCGACGGATATCTC257370.12093667207577552No Hit
TTGACATGATTTCCGGACTGTTGAC253400.11907119207367416No Hit
TCCTCGGTAGTATAGTGGTGAGTAT253260.11900540688468317No Hit
CGGACCTCGGATCAGCCTG251590.11822068355886219No Hit
CACCCCTCCCCGCACATTGACCTCA241810.11362511821363513No Hit
TTCTTTATGCTGTAGGTAAAAGCCC236090.11093732334914652No Hit
TCCAAGGCCTGAGTTACCACTGACG235630.11072117201389045No Hit
TCCAAGGCCTGAGTTACCACTGACA228690.10746010621676616No Hit
ACGCGGGAGACCGGGGTTCGATTCC227690.10699021200968771No Hit
TCGATCTGTAGTTTGAGATCATCTG227260.106788157500644No Hit
GGCCGCGATCGTCTAGTGGTTAGGA222750.10466893462672028No Hit
CGCGGGAGACCGGGGTTCGATTCCC221010.10385131870640381No Hit
GCACTGTGGTTCAATGGTAGAATTC213290.10022373542775832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)