FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-145-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-145-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19892972
Total Bases490 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGT6802673.419634833849864Illumina RNA PCR Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCAC6413903.2242040053140375No Hit
GAGAATTCTACCACTGAACCACCAG5040022.5335681365258043No Hit
TTCCGCCTCAGAAGAAAGATTTTCA3717021.8685091398107836No Hit
AGCGAGAATTCTACCACTGAACCAC2529731.2716702159938695No Hit
GAGAATTCTACCACTGAACCACAGT1788400.8990109672903577No Hit
TGCGAGAATTCTACCACTGAACCAC1514890.7615201991939666No Hit
AGAATCATACCACTAGACCAAACAG1498160.7531101938915915No Hit
GCTTGTCAGTGGTAACTCAGGTCTT1345090.6761634209307689No Hit
AGGCGAGAATTCTACCACTGAACCA1274680.6407690112870013No Hit
TTCCGCCCCAGAAGAAAGATTTTCA1007280.5063496796758171No Hit
ACAAGTTCGGATCTACGGGA1003680.5045399953310145No Hit
CCACAAGTTCGGATCTACGGGA987910.49661257252058666No Hit
ACTGCTTGTCAGTGGTAACTCAGGT772800.38847890601766294No Hit
AGGGACGCTCGGACAGCCTTGCTCG747610.3758161425050012No Hit
TGGCTCCCCGGCGGGGAATCGAACC729090.36650632193118254No Hit
AAGAGGTCAATGTGCGGGGAGGGGT671410.3375111572066758No Hit
TTTTTTTGGCCTTTAACCTACAGCA626050.3147091344621608No Hit
CTGCTTGTCAGTGGTAACTCAGGTC472570.23755625856206908No Hit
GGAGTCTCCAGATCAGGTCATTGTT465460.23398213198108356No Hit
GCGAGAATTCTACCACTGAACCACC427330.21481455862904747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG411600.20690724342245093No Hit
TGGCGAGAATTCTACCACTGAACCA408450.20532376962074847No Hit
ACTTGTCAGTGGTAACTCAGGTCTT396580.1993568381838571No Hit
GCCGGATCCTAGCCACTAGACCATA392500.1973058625930806No Hit
GCTCGTCAGTGGTAACTCAGGTCTT371410.18670412847311102No Hit
GAGTGGATAACCCTCCCCAAGTCCG360190.1810639455984757No Hit
AGTGGATAACCCTCCCCAAGTCCGG297720.1496608953151897No Hit
TCACAAGTTCGGATCTACGGGA288770.1451618189579717No Hit
GGGCGAGAATTCTACCACTGAACCA286030.14378444809553848No Hit
AAGCCTACGACACGGGGTATTCCCA284420.142975117041335No Hit
AACCCTTTCTTTGAAATACAGCGTC280120.14081354962948725No Hit
GCCCAGTTCAATTGTAAAAGACTAC275670.1385765787032727No Hit
TTTCATTTGTATCCTCACGGTTGAC272630.1370484008121059No Hit
ACTCTTCATTTATCTACTCGTTAA271760.13661106042877857No Hit
AGTTTTGGCTTAACAAGTCTGGAGG268510.1349773176174983No Hit
AGGGACTCTCGGACAGCCTTGCTCG267250.1343439280968173No Hit
AGTGAATCCATGTACGATTTTCGAT255170.12827143173981245No Hit
GAGGGACTCTCGGACAGCCTTGCTC247460.12439569110135981No Hit
GAGGGACGCTCGGACAGCCTTGCTC233150.11720219583076878No Hit
GGAATCATACCACTAGACCAAACAG232670.11696090458479508No Hit
TAAGAGGTCAATGTGCGGGGAGGGG224240.11272322707738192No Hit
GAGAATCATACCACTAGACCAAACA215080.10811858580005039No Hit
TGCTTGTCAGTGGTAACTCAGGTCT214570.10786221385120333No Hit
AGGCTGATCCGAGGTCCG204860.1029810930211936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)