FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-145-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-145-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19892972
Total Bases490 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATT15471397.77731452092729No Hit
GGAAGAGCACACGTCTGAACTCCAG6390303.2123405190536634Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTC5388792.708891361230489No Hit
TCCAAGACCTGAGTTACCACTGACA3750531.8853542849203226No Hit
TGAAAATCTTTCTTCTGAGGCGGAA3737691.8788997440905262No Hit
GAGGTCCGGACTTGGGGAGGGTTAT3053241.534833508034898No Hit
GGCTGTTTGGTCTAGTGGTATGATT2250911.1315101634888944No Hit
GAGACTCTGCACGGTGGATCTCTCG1494770.7514060744669022No Hit
TATTAATGCTGTAGGTTAAAGGCCA1111550.5587651759626465No Hit
TCCAAGACCTGAGTTACCACTGACG1072690.5392306388406921No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1012840.5091446366083459No Hit
TCCCGTAGATCCGAACTTGT1004530.5049672819124261No Hit
TCCCGTAGATCCGAACTTGTGG990670.4979999971849355No Hit
TAGTAACAATGACCTGATCTGGAGA800460.4023833140668976No Hit
CGGACCTCGGATCAGCCTGGATCAC759220.38165237451699024No Hit
GCGGGAGACCGGGGTTCGATTCCCC743360.3736797095979424No Hit
TCCCATATGGTCTAGTGGCTAGGAT698690.3512245430195146No Hit
CACCCCTCCCCGCACATTGACCTCT670330.336968251903235No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC640000.32172166129827157No Hit
GAGGTCCGGACCTGGGGAGGGTTAT595970.2995882163811421No Hit
TGACTGTAGTCTTTTACAATTGAAC527960.2654002629672429No Hit
AGTGATTGATAGGCGGGATGGATTC449930.2261753547936427No Hit
TAAAAGACGCTGTATTTCAAAGAAA427720.21501060776640113No Hit
TCCAAGGCCTGAGTTACCACTGACA418910.2105819080225921No Hit
TCACATCGAAAATCGTACATGGATT408490.20534387722457964No Hit
GCACTTGTGGTTCAGTGGTAGAATT384230.19314861550099202No Hit
TCCAAGGCCTGAGTTACTACTGACG366420.18419570489517603No Hit
CACAACTTTCAGCGACGGATATCTC361000.1814711245760563No Hit
TTACGTATCTGTTGTCTCAATCTGG351010.17644925051922858No Hit
TCCAAGGCCTGAGTTACCACTGACG348260.17506685275583758No Hit
AGGGGTCAACCGTGAGGATACAAAT344820.17333759882635938No Hit
GGCCGCGATCGTCTAGTGGTTAGGA329770.16577211288489221No Hit
TAGAGCCTCCAGACTTGTTAAGCCA324630.16318828579259045No Hit
TCCTCGGTAGTATAGTGGTGAGTAT297800.14970111052285198No Hit
TCCCGTAGATCCGAACTTGTGA289100.14532770668957862No Hit
TTAATTGTTTACTCGCGATGGAAAA286540.14404082004438554No Hit
TTAACGAGTAGATAAATGAAGAGT271400.13643009199429829No Hit
TCTTTTATGTGCGGAACCTGTCTAC269690.13557049193051696No Hit
ATAGCATGAAGTCAAACAAGAGAAC264930.13317768707461106No Hit
TAGCAAAGATTGCGTCTGATCCACT263990.13270515838457925No Hit
TGAGACCATGGACATATCCAACTTC258980.13018668100472872No Hit
TCTTCTGTTCTGCATATAAATTAGT254920.1281457592158678No Hit
GGAAGAGCACACGTCGGAACTCCAG251460.126406451484474Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGATTGGTTCTCATGTGACCTCATA249800.1255719859254816No Hit
TTGGATCCACATATCAAACACTTGA247390.12436050279465531No Hit
TAGTCCTGAGTTACCACTGACAAGA247150.12423985717166847No Hit
TGATCTTTCGTCGCGTGGTTGCTCG246580.12395332381707469No Hit
AGGTTTTGGTCTGGCTGCACTGGAA238730.12000720656521308No Hit
AGACTCTGCACGGTGGATCTCTCGG234850.1180567689935923No Hit
TCACGTGTAACTGTGCATTTCCTTG230040.1156388296328975No Hit
AGGTTTTGGTCTGGCTGCACTCGAA228620.11492500969689196No Hit
AAAGGAAAGGTTAACCGCTGAAAAA220330.11075770880288778No Hit
CACCCCTCCCCGAACATTGACCTCA210210.10567048503360886No Hit
TATAACAGACTGTCTGATTGGATCT208130.10462488963438948No Hit
CGGACCTCGGATCAGCCT206540.10382561238210157No Hit
GAGAGACTCTGCACGGTGGATCTCT201270.10117643557734862No Hit
GGTGACCGCCTGGGAATACCCCGTG200880.10098038643999499No Hit
TCTGAACTACCAATGTAAACTTTAC200470.10077428350072579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)