FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-140-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-140-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17551962
Total Bases434 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCAC3937852.2435383577061074No Hit
TCGGACTGTAGAACTCTGAACGTGT3167091.8044079630527916Illumina RNA PCR Primer (100% over 25bp)
TTCCGCCTCAGAAGAAAGATTTTCA2756221.5703201727533367No Hit
AGCGAGAATTCTACCACTGAACCAC1746110.9948232567960208No Hit
ACTTGGTAGACAGGTTCCGCACATA1667860.9502413462381015No Hit
ACTGCTTGTCAGTGGTAACTCAGGT1487190.8473069848259698No Hit
GCTTGTCAGTGGTAACTCAGGTCTT1454770.8288361153015258No Hit
AAGAGGTCAATGTGCGGGGAGGGGT1343400.7653845194058647No Hit
AGGCGAGAATTCTACCACTGAACCA1290770.7353992676146405No Hit
TTCCGCCCCAGAAGAAAGATTTTCA1093400.6229503003709785No Hit
TGCGAGAATTCTACCACTGAACCAC1031480.5876721930004178No Hit
AGAATTCTACCACTGAACCACCAGT881350.5021375957855879No Hit
TTCCGTCTCAGAAGAAAGATTTTCA872100.49686752968129716No Hit
TGGCTCCCCGGCGGGGAATCGAACC802360.4571340799393253No Hit
AGAATCATACCACTAGACCAAACAG757880.43179218368863836No Hit
CTGCTTGTCAGTGGTAACTCAGGTC504160.287238543474513No Hit
TTTTTTTGGCCTTTAACCTACAGCA501410.2856717670651292No Hit
AGGGACTCTCGGACAGCCTTGCTCG496830.28306237217241015No Hit
GAGAATTCTACCACTGAACCACCAG495540.2823274116021901No Hit
GAGGGACTCTCGGACAGCCTTGCTC458180.2610420419096167No Hit
AGTGGATAACCCTCCCCAAGTCCGG453780.2585351996546027No Hit
ACTTGTCAGTGGTAACTCAGGTCTT444520.25325943618155056No Hit
AGAATTCTACCACTGAACCACAGTG415710.23684531677997026No Hit
GCTCGTCAGTGGTAACTCAGGTCTT415380.2366573036108442No Hit
AGTGAATCCATGTACGATTTTCGAT409890.23352944816083807No Hit
GGAGTCTCCAGATCAGGTCATTGTT393520.22420285549843372No Hit
ATGAGGTCAATGTTCGGGGAGGGGT391850.22325139491528068No Hit
ACTTTGTAAAGTTTACATTGGTAGT388770.22149660533677093No Hit
TGGCGAGAATTCTACCACTGAACCA387040.2205109605410495No Hit
ACAAGTTCGGATCTACGGGA381850.21755402615388522No Hit
AATCAGAATCAAGTTTTCTTCAAGA367880.2095948019942158No Hit
ACTGCTCGTCAGTGGTAACTCAGGT363760.20724748606452087No Hit
CCACAAGTTCGGATCTACGGGA359860.20502551224757665No Hit
AAAGAGGTCAATGTGCGGGGAGGGG335550.19117520878862432No Hit
GAGTGGATAACCCTCCCCAAGTCCG291960.16634037835770155No Hit
GGGCGAGAATTCTACCACTGAACCA276960.15779432521560838No Hit
GATAACCCTCCCCAAGTCCGGACCT269540.15356687759465296No Hit
GCGAGAATTCTACCACTGAACCACC260210.148251232540271No Hit
TTTCATTTGTATCCTCACGGTTGAC257590.1467585219247854No Hit
AAGCTTCAAGAGCACTTAGACCAAA251140.14308371907368533No Hit
GCTTGGTAGACAGGTTCCGCACATA249050.14189296900255366No Hit
TAAGAGGTCAATGTGCGGGGAGGGG248300.14146566634544902No Hit
CCAGGTCAACAGTCCGGAAATCATG247280.1408845347317867No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG245910.1401039952114755No Hit
ACTGTTCTCGCTGTTATTATATTCA245300.13975645571703038No Hit
AGAGGTCAATGTGCGGGGAGGGGTG242350.1380757319324187No Hit
GAGAATTCTACCACTGAACCACAGT232880.13268032371537725No Hit
ACTCTTCATTTATCTACTCGTTAA225850.12867507347611623No Hit
GCTGCTTGTCAGTGGTAACTCAGGT224700.12801987606855575No Hit
ACTGCTCGTCAGTAGTAACTCAGGC218380.12441913901135382No Hit
AGAGTGGATAACCCTCCCCAAGTCC211360.12041958614085423No Hit
GCTTGTCAGTGGTAACTCAGGCCTT201020.11452850684157131No Hit
ACTCGTCGTATGTCCCTTAAATTCC192460.10965155918181682No Hit
AAGAGTGGATAACCCTCCCCAAGTC187000.1065407958380949No Hit
TGGATAACCCTCCCCAAGTCCGGAC184520.10512784838526883No Hit
ACTTGGTCGAGAGGTTCCACTCATA183630.10462078256550464No Hit
AGTTCGGCGGGTGATCCAGGCTGAT183340.10445555887142419No Hit
ACTTGGTTGACAGGATCCGCACATA182250.10383454567643208No Hit
AGGGACGCTCGGACAGCCTTGCTCG180880.1030540061561209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)