FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-140-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-140-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17551962
Total Bases433.9 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATT7987964.551035377127639No Hit
TCCAAGACCTGAGTTACCACTGACA4747822.7050081352728546No Hit
GAGGTCCGGACTTGGGGAGGGTTAT3755812.1398234567736645No Hit
GCACTGTGGTTCAGTGGTAGAATTC3744462.1333569432294803No Hit
GGAAGAGCACACGTCTGAACTCCAG3070121.74916057817354Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGAGGCGGAA2771901.5792536469712046No Hit
TCTTTTATGTGCGGAACCTGTCTAC2321641.3227239211206132No Hit
GAGACTCTGCACGGTGGATCTCTCG1470170.8376100631940749No Hit
TCCAAGACCTGAGTTACCACTGACG1393310.7938200868939893No Hit
CACCCCTCCCCGCACATTGACCTCT1311010.7469307419877048No Hit
CGGACCTCGGATCAGCCTGGATCAC1277620.7279072276934054No Hit
GGCTGTTTGGTCTAGTGGTATGATT1102300.6280209585686205No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1100540.6270182216666148No Hit
TATTAATGCTGTAGGTTAAAGGCCA899940.5127290043130222No Hit
TGAAAATCTTTCTTCTGAGACGGAA875390.4987419640037963No Hit
GCGGGAGACCGGGGTTCGATTCCCC777820.4431527369988609No Hit
GAGGTCCGGACCTGGGGAGGGTTAT777220.4428108948731771No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC762530.4344414601626872No Hit
TCACATCGAAAATCGTACATGGATT698360.39788144482081267No Hit
TAGTAACAATGACCTGATCTGGAGA670730.38213961493307697No Hit
TTGACATGATTTCCGGACTGTTGAC528360.30102617587708996No Hit
TCCAAGGCCTGAGTTACTACTGACG516180.2940867807257103No Hit
TCTAAGGCCTGAGTTACCACTGACA514170.2929416096046698No Hit
AGGTTTTGGTCTAAGTGCTCTTGAA508270.2895801620354465No Hit
TCTGAACTACCAATGTAAACTTTAC464570.26468266054814843No Hit
TTGGTTGAATATAATAACAGCGAGA458020.2609508840094344No Hit
CACCCCTCCCCGAACATTGACCTCA457530.26067171294012603No Hit
TCTTTTATGAGTGGAACCTCTCGAC436180.2485078306345467No Hit
TCCAAGGCCTGAGTTACCACTGACG432900.246639093680809No Hit
TCCCGTAGATCCGAACTTGT381800.2175255393100783No Hit
TTTTATGATGAATCTTTTAGACTAG378270.21551436813730568No Hit
TCCCGTAGATCCGAACTTGTGG361200.20578895966160363No Hit
AGTGATTGATAGGCGGGATGGATTC354700.2020856699666966No Hit
TCCAAGGCCTGAGTTACCACTGACA346600.1974708012699663No Hit
TCTTTTATGTGCGGATCCTGTCAAC339780.1935851957746946No Hit
TAAGACTCTTCCCGAAACCAGTCGG328810.18733518224344378No Hit
AGGGGTCAACCGTGAGGATACAAAT315200.17958106335918458No Hit
TCTTTTATGTGCGGAACCTGTCAAC313160.1784188001318599No Hit
TGACTGTAGTCTTTTACAATTGAAC302930.17259039188895234No Hit
TTAATTGTTTACTCGCGATGGAAAA298170.1698784443585281No Hit
TTATGGAATTTAAGGGACATACGAC295960.16861932586225972No Hit
ATAGCATGAAGTCAAACAAGAGAAC285730.1627909176193522No Hit
ACCCCTCCCCGCACATTGACCTCTT284790.16225536495578102No Hit
AAAGGAAAGGTTAACCGCTGAAAAA272960.1555153777110502No Hit
AGACTCTGCACGGTGGATCTCTCGG268620.15304271966860458No Hit
TGTTGCTGGCAAAAGTAGAAATCTG262490.14955023261786915No Hit
CCACCCCTCCCCGCACATTGACCTC253920.14466758758935325No Hit
TTGCAAGTTCCCTGAAATCTCCGAA249210.141984126902736No Hit
TTAACGAGTAGATAAATGAAGAGT225730.1286067050509795No Hit
TTTCAAAGGGTACTGTGCATGGAAA221860.12640182334031946No Hit
AGGTTTTGGTCTGGCTGCACTGGAA218390.12442483638011523No Hit
TGATCTTTCGTCGCGTGGTTGCTCG216650.12343349421563242No Hit
TTACGTATCTGTTGTCTCAATCTGG209670.11945673082017838No Hit
CGGCCTGAAAAAAAAAAAAAAAAAA207580.11826598074904675No Hit
CACAAGTCCATAATAAGTGGGCTCC206200.11747974385997419No Hit
TTGGATCCACATATCAAACACTTGA198180.11291045411333503No Hit
TATGGTCTCTTGTTATGGACAAAGC191960.10936669074374705No Hit
TACCACCCCTCCCCGCACATTGACC191070.10885962492398285No Hit
TGCTTACAATGTCGTCTGCGACCGA190700.10864882227981122No Hit
GGCCGCGATCGTCTAGTGGTTAGGA188630.10746946694620238No Hit
TATGAGGAGAATGTTTGGGCTCTGG187080.10658637478818608No Hit
TGATTGGTTCTCATGTGACCTCATA184040.10485437468472185No Hit
TGAAAAGTATCGTCTCCTCTTTCAT183040.10428463780858231No Hit
TCTGTCTGGATTTGAGTCAAGTGAA181440.10337305880675904No Hit
GCACTTGTGGTTCAGTGGTAGAATT178370.10162396659701063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)