FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-178-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-178-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17687362
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT6844333.8696160569337583Illumina RNA PCR Primer (100% over 50bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC5191982.9354179554870874No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3735202.1117903280319585No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3097381.751182567530421Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT2060681.1650578531722255No Hit
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1957361.1066432631389578Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1553920.8785481972947691No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT1514080.8560236399300246Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT1404620.7941376447205637No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1355980.7666377835202333No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT1216670.6878753315502899Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT827590.4678990569650805Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT609300.3444832530707519Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT578790.3272336485226005No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA560790.3170568906770835No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC545420.30836707022788357No Hit
CCAGGTCAACAGTCCGGAAATCATGTCAAGATCGTCGGACTGTAGAACTC448030.2533051565292778Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT419940.2374237605359126Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC336350.19016402785220315No Hit
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC284190.16067404511763822Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT269050.1521142610186867Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT266080.15043509597417637Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT265210.14994321934497637No Hit
GCTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT263490.14897077359529362No Hit
AAGCACAAAGAACTCAAAGAAGGAAGTAGATCGTCGGACTGTAGAACTCT254560.14392197095304546Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT247880.14014526304148692No Hit
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC237360.13419751345621805Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG234720.13270492230554223No Hit
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG227960.12888298435911472Illumina RNA PCR Primer (100% over 33bp)
GGCAGTCTGCTCAGAATTTCCACCAGAAGATCGTCGGACTGTAGAACTCT227570.12866248793912852Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT226450.12802926745096302Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC219580.12414513820659066No Hit
AATCATACCACTCTTAGCCCGTCATTTAAGATCGTCGGACTGTAGAACTC216000.12212109414620449Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT213200.12053804292579075Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCAGTCTGCTCAGAATTTCCACCAGAAGATCGTCGGACTGTAGAACTCT212510.12014793387504592Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACACGCTTTGTCCATAACAAGAGACCATAGATCGTCGGACTGTAGAACTC201620.11399099537850813Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAA200440.11332385236419089No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT194420.1099202922403013No Hit
ACTTTTTATTTACGGAATCCACAATCTTAGATCGTCGGACTGTAGAACTC188570.10661284594050825Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCCGGAGATCCTAACCACCTAGACTACGTTGGAGATCGTCGGACTGTAG185520.10488845086112897No Hit
ATTGTAAGCTTATATATACGAGTTAGATCAGATCGTCGGACTGTAGAACT182300.10306794195765316No Hit
AGTTTTGGCTTAACAAGTCTGGAGGCTCTAGATCGTCGGACTGTAGAACT181030.10234991515410835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)