FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-173-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-173-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19686271
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2810291.4275380035152416No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG2238671.13717321071116Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA2195031.1150054776752794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC2184911.109864839308572Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1798460.9135605214415671Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1344690.6830597831351606No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1220960.6202088755153273No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT1218440.618928795605831No Hit
GCACTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA1005650.5108382384861003Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG939000.4769821567527949Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC938630.47679420851211485No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG890810.45250316832476806No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG881200.4476215937492682Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC800430.40659300077703897Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCAAGACCTGAGTTACCACTGACAAGTAGATCGGAAGAGCACACGTCTG739540.3756628159797252Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT665430.3380172913397362No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC628480.3192478656826374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT612760.311262605294827No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC607730.3087075251580149No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG601610.30559875966352384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT526270.26732843411532836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC522410.2653676767936396Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT512590.2603794288923484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC488370.2480764386510782No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG469750.23861807043091096No Hit
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACT428780.2178066125372347TruSeq Adapter, Index 7 (97% over 35bp)
TTGACATGATTTCCGGACTGTTGACCTGGAGATCGGAAGAGCACACGTCT427460.2171360944894033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATG404580.20551378166032563TruSeq Adapter, Index 7 (97% over 35bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA401240.2038171678120249Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGA390480.19835142978576287Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT387280.19672593148798975No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA361610.18368638733054116Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC343750.17461407495609504No Hit
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA343580.17452772035902583Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTTTG341630.17353718233382034TruSeq Adapter, Index 7 (97% over 35bp)
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC332770.16903658392186108No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC316020.16052811626945498No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC303610.15422423068340368No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCCGTC290550.1475901657556172RNA PCR Primer, Index 32 (100% over 34bp)
TCCAAGACCTGAGTTACCACTGACGAGCAGTAGATCGGAAGAGCACACGT284560.14454743612947318No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT282430.14346546382501796No Hit
CACAACTTTCAGCGACGGATATCTCGGCTAGATCGGAAGAGCACACGTCT280910.14269335213357573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG266210.13522621932818055Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC245780.12484842863333537No Hit
TACGAACATACTTTCGGCACAATCTGAAATAGATCGGAAGAGCACACGTC244840.12437093850836453No Hit
TCCAAGACCTGAGTTACCACTGACGAGTAGATCGGAAGAGCACACGTCTG242770.12331944429699257Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAAGACCTGAGTTACCACTGACAAGCAAGATCGGAAGAGCACACGTCT237320.12055101750859776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCGCGTATG235340.11954524043685064TruSeq Adapter, Index 7 (97% over 35bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACG229850.11675649491973364No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGCAGATCGGAAGAGCACACGT222130.11283498027635605No Hit
AGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAGC221880.11270798822184253No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGTAGATCGGAAGAGCACACGT220400.11195619525912247No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG219940.11172252987881758No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACTAGATCGGAAGAGCACACGTC215130.10927920274997739No Hit
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT211530.10745051716498265Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTAACTCGTATATATAAGCTTACAATAGATCGGAAGAGCACACGTC208270.10579454077412628No Hit
TCCCGTAGATCCGAACTTGCAGATCGGAAGAGCACACGTCTGAACTCCAG202250.10273657210144065Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCATATGGTCTAGTGGCTAGGATCCGAGATCGGAAGAGCACACGTCTG202170.10269593464399632Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTCC200240.1017155559831519Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCAAGACCTGAGTTACCACTGACAAGATCGGAAGAGCACACGTCTGAAC199730.10145649219194433Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTAACAATGACCTGATCTGGAGACTCTAGATCGGAAGAGCACACGTCT198670.10091804588080698Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCGCGTTTG197710.10043039639147505TruSeq Adapter, Index 7 (97% over 35bp)
TCTTTTATGTGCGGAACCTGTCAACCAAGTAGATCGGAAGAGCACACGTC197030.10008497800319827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)