FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-145-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-145-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21295979
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG4746752.228941904948347Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4514172.1197287995071745No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC3541191.6628444271099254Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1732960.8137498632957892No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA1682370.789994205009312Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1504930.7066733114265373No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1405680.6600682692258477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG1286430.6040717827529789Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG1267470.5951686935829529No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1031990.4845938287223142No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC961030.45127298444462216Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG955080.4484790297736488Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC938570.4407263925269648Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG893040.4193467696413487No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT732900.3441494753540093No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC614560.2885802995955246No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT587880.27605211293643744No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC492300.2311704007596927No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGGTTG488900.22957385523342225TruSeq Adapter, Index 7 (97% over 35bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC449290.21097409985237117No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT433120.20338111715831425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC407410.1913084155464278No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC379390.17815100212110463No Hit
TCCAAGACCTGAGTTACCACTGACAAGTAGATCGGAAGAGCACACGTCTG373860.17555426777984706Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC372520.1749250410136111No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG353870.1661675192298039Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT341100.16017108206201744No Hit
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACC340510.15989403445598813TruSeq Adapter, Index 7 (97% over 35bp)
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA326400.15326837052196568Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCGCGGTTG322870.15161078060792602TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCGCGGGTG302080.14184837428699568TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGGGTG301360.14151028229319723TruSeq Adapter, Index 7 (97% over 35bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT293890.13800257785753828No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACG281830.13233953696141418No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC277520.13031568072075955No Hit
TCCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTCC277090.13011376466890767Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAAAGACGCTGTATTTCAAAGAAAGGGTTAGATCGGAAGAGCACACGTC267370.12554952275262854No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGCAGATCGGAAGAGCACACGTC263870.1239060200049972No Hit
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT257850.12107919527907124Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT252540.11858576682480762Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGGTTGGCGGG245390.11522832549750354TruSeq Adapter, Index 7 (96% over 31bp)
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT231800.10884683911455773Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA221360.10394450520448016Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)