FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-140-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-140-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18506972
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT2991971.616671814276263Illumina RNA PCR Primer (100% over 50bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2579471.3937828403263375Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2440161.3185085058755155No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT1555210.8403373604282752No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1421470.7680727025469104Illumina RNA PCR Primer (100% over 45bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC1375700.7433414823343332No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT1354660.7319727938206207Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1111860.6007789929114281No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1055600.570379638549191No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1024360.5534995135887167Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG810970.4381970211010207Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA810860.4381375840413007Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA792600.42827103212778406No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG696280.3762257812893433Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA670670.3623877531127188Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC616110.3329069714916087No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT506980.27394000488032294No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG506170.27350233198602125No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT469300.253580110241697No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC461760.24950596996634566No Hit
AGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG454470.2455669139176306No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT451460.24394049982892932Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTT427440.23096160733371185No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT412630.22295921774777636Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT409810.22143546767131866No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT386120.20863488635526115Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTGA385740.20842955833077392Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC372860.2014700189744708Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC370950.20043797548296932No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT359750.19438620212966226No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT357110.19295971269638273Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC355660.1921762241818921Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT337570.1824015295424881Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTGCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC335670.18137488942005206No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT308310.16659127165697338No Hit
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT305150.16488380703229033No Hit
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG296770.16035578375544093Illumina RNA PCR Primer (100% over 33bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC267310.14443745848861717No Hit
ACCGTGCAGAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC259240.14007693965279677Illumina RNA PCR Primer (100% over 30bp)
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA249370.13474381438519495Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT240190.12978352158310932No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA234210.12655230688196858No Hit
GCTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT233110.12595793628476878No Hit
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC232900.12584446553439427Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTGTTCTCGCTGTTATTATATTCAACCAAGATCGTCGGACTGTAGAACT228380.12340214271680963No Hit
CCAGGTCAACAGTCCGGAAATCATGTCAAGATCGTCGGACTGTAGAACTC223200.12060319754090512Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGCTTCAAGAGCACTTAGACCAAAACCTGATCGTCGGACTGTAGAACTC217350.11744222663761528Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG213290.11524845879704146Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC211460.1142596422580636Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC207010.11185514302393715No Hit
ATGAGGTCAATGTTCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA204600.11055293107916303Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGAGATCGTCGGACTGTAGAAC202550.10944524042074522No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAA191600.10352855129407447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)