FastQCFastQC Report
Wed 1 Nov 2023
sRNA-ACR-140-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-140-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18506972
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC2670481.4429589021910227Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2544321.3747899980612712No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC1595070.86187518952317No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT1414720.7644254284277299No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG1397600.7551748605876748Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG1168320.6312864146549744No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1155190.6241917910720349No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1097170.5928414437542782No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC1059770.572632843449485Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC842490.45522844039532784Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA838880.4532778241626993Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG821860.44408129001329877No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA712860.3851845671998639Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA706630.38181826827208687Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT643750.34784188358852003No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTC621160.3356356728696623RNA PCR Primer, Index 29 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATG555020.29989778987075794TruSeq Adapter, Index 23 (97% over 38bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC524500.2834067074829961No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC520590.2812939901784041No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC478430.2585133861984554No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT472360.25523354117572555No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG464880.2511918211147669Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAAGACCTGAGTTACCACTGACAAGTAGATCGGAAGAGCACACGTCTG425420.22987012678249039Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC422420.22824911606285458No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG400580.2164481580239058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA396280.2141247093257611Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT385780.20845117180703573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT383570.2072570272435707No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC371770.20088105174633644No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT368990.19937891514614062Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG366830.1982117874280028Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCAAGACCTGAGTTACCACTGACGAGCAGTAGATCGGAAGAGCACACGT347430.18772925144102448No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC344300.18603799692353779Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA332020.17940265971116182No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC318060.1718595564957898No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCGCGTATGCCGTC312590.16890391361698717TruSeq Adapter, Index 23 (97% over 34bp)
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAC305000.16480275649630852TruSeq Adapter, Index 23 (97% over 37bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT277030.14968953322023723No Hit
GAGACTCTGCACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC264710.14303258253159945Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGTCCGGACTTGGGGAGGGTTATCAGATCGGAAGAGCACACGTCTGAA257610.13919619049512802Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC247510.13373878773902073No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACG244640.1321880208172358No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCGCGTATG243930.13180438161358865TruSeq Adapter, Index 23 (97% over 38bp)
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT243600.13162607043442873Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTTTATGTGCGGAACCTGTCTACCAAGCAGATCGGAAGAGCACACGTC240010.12968626093993119No Hit
TTGGTTGAATATAATAACAGCGAGAACAGTAGATCGGAAGAGCACACGTC234900.12692513934748484No Hit
TTGACATGATTTCCGGACTGTTGACCTGGAGATCGGAAGAGCACACGTCT228410.123418352824006Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTTTTGGTCTAAGTGCTCTTGAAGCTTAGATCGGAAGAGCACACGTCT223970.121019256958945Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCCCTCCCCGAACATTGACCTCATAGATCGGAAGAGCACACGTCTGAA218660.11815006798518958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA218650.11814466461612413Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT217560.11755569738798978Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAGC216490.11697753689798635No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGCAGATCGGAAGAGCACACGT215630.11651284715835739No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTTTG210090.11351938069609657TruSeq Adapter, Index 23 (97% over 38bp)
TCCAAGGCCTGAGTTACTACTGACGAGCAGTAGATCGGAAGAGCACACGT208600.11271427870534413No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTAGATCGGAAGAGCACACG198310.10715421193699326No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)