Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10714287 total bases: 1082142987 Q20 bases: 1057606642(97.7326%) Q30 bases: 1024453719(94.669%) Read2 before filtering: total reads: 10714287 total bases: 1082142987 Q20 bases: 1019174291(94.1811%) Q30 bases: 975661583(90.1601%) Read1 after filtering: total reads: 9977161 total bases: 814669609 Q20 bases: 801251433(98.3529%) Q30 bases: 777980893(95.4965%) Read2 after filtering: total reads: 9977161 total bases: 813926528 Q20 bases: 797362294(97.9649%) Q30 bases: 770214415(94.6295%) Filtering result: reads passed filter: 19954322 reads failed due to low quality: 1357312 reads failed due to too many N: 790 reads failed due to too short: 116150 reads with adapter trimmed: 5632589 bases trimmed due to adapters: 89240316 reads with polyX in 3' end: 149266 bases trimmed in polyX tail: 2999071 Duplication rate: 21.0772% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L001.fastp-trim.report.html fastp --in1 lib_212394_sample_1703733_S114_L001_R1_001.fastq.gz --in2 lib_212394_sample_1703733_S114_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 24 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10703360 total bases: 1081039360 Q20 bases: 1055843489(97.6693%) Q30 bases: 1022448667(94.5802%) Read2 before filtering: total reads: 10703360 total bases: 1081039360 Q20 bases: 1018297552(94.1962%) Q30 bases: 974360828(90.1319%) Read1 after filtering: total reads: 9963756 total bases: 813747652 Q20 bases: 800153849(98.3295%) Q30 bases: 776691415(95.4462%) Read2 after filtering: total reads: 9963756 total bases: 812991348 Q20 bases: 796233993(97.9388%) Q30 bases: 768851147(94.5706%) Filtering result: reads passed filter: 19927512 reads failed due to low quality: 1347964 reads failed due to too many N: 932 reads failed due to too short: 130312 reads with adapter trimmed: 5600308 bases trimmed due to adapters: 88739837 reads with polyX in 3' end: 154130 bases trimmed in polyX tail: 3139182 Duplication rate: 21.0272% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L002.fastp-trim.report.html fastp --in1 lib_212394_sample_1703733_S114_L002_R1_001.fastq.gz --in2 lib_212394_sample_1703733_S114_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212394_sample_1703733_S114_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 24 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 8939299 total bases: 902869199 Q20 bases: 883499279(97.8546%) Q30 bases: 856490305(94.8632%) Read2 before filtering: total reads: 8939299 total bases: 902869199 Q20 bases: 871733294(96.5514%) Q30 bases: 842104897(93.2699%) Read1 after filtering: total reads: 8628266 total bases: 700582314 Q20 bases: 689129054(98.3652%) Q30 bases: 668753468(95.4568%) Read2 after filtering: total reads: 8628266 total bases: 700097655 Q20 bases: 687504533(98.2012%) Q30 bases: 666266675(95.1677%) Filtering result: reads passed filter: 17256532 reads failed due to low quality: 547666 reads failed due to too many N: 828 reads failed due to too short: 73572 reads with adapter trimmed: 5518475 bases trimmed due to adapters: 85403331 reads with polyX in 3' end: 97350 bases trimmed in polyX tail: 1701974 Duplication rate: 22.7697% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L001.fastp-trim.report.html fastp --in1 lib_212395_sample_1703674_S98_L001_R1_001.fastq.gz --in2 lib_212395_sample_1703674_S98_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 21 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 8923648 total bases: 901288448 Q20 bases: 881266550(97.7785%) Q30 bases: 853968767(94.7498%) Read2 before filtering: total reads: 8923648 total bases: 901288448 Q20 bases: 869929725(96.5207%) Q30 bases: 839972335(93.1968%) Read1 after filtering: total reads: 8609252 total bases: 699008402 Q20 bases: 687301789(98.3253%) Q30 bases: 666634543(95.3686%) Read2 after filtering: total reads: 8609252 total bases: 698495550 Q20 bases: 685722873(98.1714%) Q30 bases: 664281933(95.1018%) Filtering result: reads passed filter: 17218504 reads failed due to low quality: 549618 reads failed due to too many N: 682 reads failed due to too short: 78492 reads with adapter trimmed: 5499444 bases trimmed due to adapters: 85301771 reads with polyX in 3' end: 99444 bases trimmed in polyX tail: 1751360 Duplication rate: 22.6627% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L002.fastp-trim.report.html fastp --in1 lib_212395_sample_1703674_S98_L002_R1_001.fastq.gz --in2 lib_212395_sample_1703674_S98_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212395_sample_1703674_S98_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 22 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10603958 total bases: 1070999758 Q20 bases: 1047404341(97.7969%) Q30 bases: 1016739870(94.9337%) Read2 before filtering: total reads: 10603958 total bases: 1070999758 Q20 bases: 1037050961(96.8302%) Q30 bases: 1002014483(93.5588%) Read1 after filtering: total reads: 10279252 total bases: 834514217 Q20 bases: 821267383(98.4126%) Q30 bases: 797656562(95.5833%) Read2 after filtering: total reads: 10279252 total bases: 833843817 Q20 bases: 819185322(98.2421%) Q30 bases: 794189298(95.2444%) Filtering result: reads passed filter: 20558504 reads failed due to low quality: 565740 reads failed due to too many N: 898 reads failed due to too short: 82774 reads with adapter trimmed: 6405338 bases trimmed due to adapters: 102171841 reads with polyX in 3' end: 107426 bases trimmed in polyX tail: 1761853 Duplication rate: 24.7503% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L001.fastp-trim.report.html fastp --in1 lib_212396_sample_1703690_S106_L001_R1_001.fastq.gz --in2 lib_212396_sample_1703690_S106_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 25 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10583607 total bases: 1068944307 Q20 bases: 1044872750(97.7481%) Q30 bases: 1013887379(94.8494%) Read2 before filtering: total reads: 10583607 total bases: 1068944307 Q20 bases: 1034795601(96.8054%) Q30 bases: 999469433(93.5006%) Read1 after filtering: total reads: 10255260 total bases: 832594497 Q20 bases: 819069355(98.3755%) Q30 bases: 795127194(95.4999%) Read2 after filtering: total reads: 10255260 total bases: 831921113 Q20 bases: 817094715(98.2178%) Q30 bases: 791909527(95.1905%) Filtering result: reads passed filter: 20510520 reads failed due to low quality: 568162 reads failed due to too many N: 950 reads failed due to too short: 87582 reads with adapter trimmed: 6380444 bases trimmed due to adapters: 101902194 reads with polyX in 3' end: 109217 bases trimmed in polyX tail: 1832117 Duplication rate: 24.6266% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L002.fastp-trim.report.html fastp --in1 lib_212396_sample_1703690_S106_L002_R1_001.fastq.gz --in2 lib_212396_sample_1703690_S106_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212396_sample_1703690_S106_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 27 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10007582 total bases: 1010765782 Q20 bases: 989778126(97.9236%) Q30 bases: 960375573(95.0147%) Read2 before filtering: total reads: 10007582 total bases: 1010765782 Q20 bases: 977877189(96.7462%) Q30 bases: 945457838(93.5388%) Read1 after filtering: total reads: 9699248 total bases: 796310390 Q20 bases: 783099376(98.341%) Q30 bases: 760093283(95.4519%) Read2 after filtering: total reads: 9699248 total bases: 795622772 Q20 bases: 781498115(98.2247%) Q30 bases: 757545909(95.2142%) Filtering result: reads passed filter: 19398496 reads failed due to low quality: 541444 reads failed due to too many N: 832 reads failed due to too short: 74392 reads with adapter trimmed: 5424610 bases trimmed due to adapters: 76956646 reads with polyX in 3' end: 110098 bases trimmed in polyX tail: 2076413 Duplication rate: 19.9998% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L001.fastp-trim.report.html fastp --in1 lib_212397_sample_1703745_S115_L001_R1_001.fastq.gz --in2 lib_212397_sample_1703745_S115_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 22 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10007700 total bases: 1010777700 Q20 bases: 989320476(97.8772%) Q30 bases: 959783149(94.9549%) Read2 before filtering: total reads: 10007700 total bases: 1010777700 Q20 bases: 977644301(96.722%) Q30 bases: 944930172(93.4855%) Read1 after filtering: total reads: 9694655 total bases: 796000913 Q20 bases: 782622712(98.3193%) Q30 bases: 759439418(95.4069%) Read2 after filtering: total reads: 9694655 total bases: 795308208 Q20 bases: 781006938(98.2018%) Q30 bases: 756860960(95.1657%) Filtering result: reads passed filter: 19389310 reads failed due to low quality: 541578 reads failed due to too many N: 930 reads failed due to too short: 83582 reads with adapter trimmed: 5405689 bases trimmed due to adapters: 76836536 reads with polyX in 3' end: 113658 bases trimmed in polyX tail: 2148193 Duplication rate: 19.9264% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L002.fastp-trim.report.html fastp --in1 lib_212397_sample_1703745_S115_L002_R1_001.fastq.gz --in2 lib_212397_sample_1703745_S115_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212397_sample_1703745_S115_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 23 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 9020057 total bases: 911025757 Q20 bases: 895941043(98.3442%) Q30 bases: 872144959(95.7322%) Read2 before filtering: total reads: 9020057 total bases: 911025757 Q20 bases: 890633049(97.7616%) Q30 bases: 863314818(94.7629%) Read1 after filtering: total reads: 8851159 total bases: 716593848 Q20 bases: 707032147(98.6657%) Q30 bases: 688301116(96.0518%) Read2 after filtering: total reads: 8851159 total bases: 716077106 Q20 bases: 706500191(98.6626%) Q30 bases: 686281875(95.8391%) Filtering result: reads passed filter: 17702318 reads failed due to low quality: 295880 reads failed due to too many N: 776 reads failed due to too short: 41140 reads with adapter trimmed: 6004885 bases trimmed due to adapters: 90026818 reads with polyX in 3' end: 176339 bases trimmed in polyX tail: 2450294 Duplication rate: 27.3023% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L001.fastp-trim.report.html fastp --in1 lib_212398_sample_1703677_S99_L001_R1_001.fastq.gz --in2 lib_212398_sample_1703677_S99_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 21 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 9004200 total bases: 909424200 Q20 bases: 893945090(98.2979%) Q30 bases: 869949458(95.6594%) Read2 before filtering: total reads: 9004200 total bases: 909424200 Q20 bases: 888958563(97.7496%) Q30 bases: 861477551(94.7278%) Read1 after filtering: total reads: 8832861 total bases: 715346235 Q20 bases: 705567240(98.633%) Q30 bases: 686584142(95.9793%) Read2 after filtering: total reads: 8832861 total bases: 714828179 Q20 bases: 705179715(98.6502%) Q30 bases: 684844722(95.8055%) Filtering result: reads passed filter: 17665722 reads failed due to low quality: 299904 reads failed due to too many N: 788 reads failed due to too short: 41986 reads with adapter trimmed: 5968700 bases trimmed due to adapters: 89369229 reads with polyX in 3' end: 177686 bases trimmed in polyX tail: 2492128 Duplication rate: 27.2183% Insert size peak (evaluated by paired-end reads): 117 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L002.fastp-trim.report.html fastp --in1 lib_212398_sample_1703677_S99_L002_R1_001.fastq.gz --in2 lib_212398_sample_1703677_S99_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212398_sample_1703677_S99_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 20 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 8315237 total bases: 839838937 Q20 bases: 826252997(98.3823%) Q30 bases: 804617797(95.8062%) Read2 before filtering: total reads: 8315237 total bases: 839838937 Q20 bases: 818339964(97.4401%) Q30 bases: 794514201(94.6032%) Read1 after filtering: total reads: 8104035 total bases: 652014684 Q20 bases: 643243128(98.6547%) Q30 bases: 626732129(96.1224%) Read2 after filtering: total reads: 8104035 total bases: 651516619 Q20 bases: 643605575(98.7857%) Q30 bases: 627367209(96.2934%) Filtering result: reads passed filter: 16208070 reads failed due to low quality: 369926 reads failed due to too many N: 656 reads failed due to too short: 51822 reads with adapter trimmed: 5619657 bases trimmed due to adapters: 91515838 reads with polyX in 3' end: 167540 bases trimmed in polyX tail: 2329064 Duplication rate: 24.9278% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L001.fastp-trim.report.html fastp --in1 lib_212399_sample_1703691_S107_L001_R1_001.fastq.gz --in2 lib_212399_sample_1703691_S107_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 16 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 8294940 total bases: 837788940 Q20 bases: 823665759(98.3142%) Q30 bases: 801723666(95.6952%) Read2 before filtering: total reads: 8294940 total bases: 837788940 Q20 bases: 816200012(97.4231%) Q30 bases: 792266730(94.5664%) Read1 after filtering: total reads: 8080702 total bases: 650423950 Q20 bases: 641398519(98.6124%) Q30 bases: 624607037(96.0308%) Read2 after filtering: total reads: 8080702 total bases: 649891248 Q20 bases: 641922709(98.7739%) Q30 bases: 625619117(96.2652%) Filtering result: reads passed filter: 16161404 reads failed due to low quality: 376092 reads failed due to too many N: 764 reads failed due to too short: 51620 reads with adapter trimmed: 5574370 bases trimmed due to adapters: 90717706 reads with polyX in 3' end: 167596 bases trimmed in polyX tail: 2339395 Duplication rate: 24.8697% Insert size peak (evaluated by paired-end reads): 117 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L002.fastp-trim.report.html fastp --in1 lib_212399_sample_1703691_S107_L002_R1_001.fastq.gz --in2 lib_212399_sample_1703691_S107_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212399_sample_1703691_S107_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 18 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10046393 total bases: 1014685693 Q20 bases: 996790626(98.2364%) Q30 bases: 969264719(95.5236%) Read2 before filtering: total reads: 10046393 total bases: 1014685693 Q20 bases: 979079989(96.491%) Q30 bases: 948544232(93.4816%) Read1 after filtering: total reads: 9649058 total bases: 785691120 Q20 bases: 774711295(98.6025%) Q30 bases: 753997322(95.9661%) Read2 after filtering: total reads: 9649058 total bases: 785001444 Q20 bases: 774764583(98.6959%) Q30 bases: 754023903(96.0538%) Filtering result: reads passed filter: 19298116 reads failed due to low quality: 736742 reads failed due to too many N: 746 reads failed due to too short: 57182 reads with adapter trimmed: 5997838 bases trimmed due to adapters: 88636986 reads with polyX in 3' end: 222957 bases trimmed in polyX tail: 3580929 Duplication rate: 25.3068% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L001.fastp-trim.report.html fastp --in1 lib_212400_sample_1703748_S116_L001_R1_001.fastq.gz --in2 lib_212400_sample_1703748_S116_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 20 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10024330 total bases: 1012457330 Q20 bases: 993967325(98.1737%) Q30 bases: 966226407(95.4338%) Read2 before filtering: total reads: 10024330 total bases: 1012457330 Q20 bases: 976977160(96.4956%) Q30 bases: 946261034(93.4618%) Read1 after filtering: total reads: 9625639 total bases: 783969835 Q20 bases: 772761479(98.5703%) Q30 bases: 751810256(95.8979%) Read2 after filtering: total reads: 9625639 total bases: 783284368 Q20 bases: 772981999(98.6847%) Q30 bases: 752100986(96.0189%) Filtering result: reads passed filter: 19251278 reads failed due to low quality: 736954 reads failed due to too many N: 842 reads failed due to too short: 59586 reads with adapter trimmed: 5952438 bases trimmed due to adapters: 88067346 reads with polyX in 3' end: 224211 bases trimmed in polyX tail: 3634543 Duplication rate: 25.2474% Insert size peak (evaluated by paired-end reads): 117 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L002.fastp-trim.report.html fastp --in1 lib_212400_sample_1703748_S116_L002_R1_001.fastq.gz --in2 lib_212400_sample_1703748_S116_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212400_sample_1703748_S116_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 19 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 9992083 total bases: 1009200383 Q20 bases: 984966909(97.5987%) Q30 bases: 953948341(94.5252%) Read2 before filtering: total reads: 9992083 total bases: 1009200383 Q20 bases: 953479561(94.4787%) Q30 bases: 914801096(90.6461%) Read1 after filtering: total reads: 9325389 total bases: 745112305 Q20 bases: 733197179(98.4009%) Q30 bases: 712418545(95.6122%) Read2 after filtering: total reads: 9325389 total bases: 744508470 Q20 bases: 730947278(98.1785%) Q30 bases: 708091761(95.1086%) Filtering result: reads passed filter: 18650778 reads failed due to low quality: 1229120 reads failed due to too many N: 838 reads failed due to too short: 103430 reads with adapter trimmed: 6510053 bases trimmed due to adapters: 117867317 reads with polyX in 3' end: 130327 bases trimmed in polyX tail: 2408475 Duplication rate: 24.2952% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L001.fastp-trim.report.html fastp --in1 lib_212401_sample_1703678_S100_L001_R1_001.fastq.gz --in2 lib_212401_sample_1703678_S100_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 23 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 9970182 total bases: 1006988382 Q20 bases: 981814402(97.5001%) Q30 bases: 950571943(94.3975%) Read2 before filtering: total reads: 9970182 total bases: 1006988382 Q20 bases: 951481266(94.4878%) Q30 bases: 912433325(90.6101%) Read1 after filtering: total reads: 9302911 total bases: 743578004 Q20 bases: 731409042(98.3635%) Q30 bases: 710344560(95.5306%) Read2 after filtering: total reads: 9302911 total bases: 742961506 Q20 bases: 729221802(98.1507%) Q30 bases: 706145311(95.0447%) Filtering result: reads passed filter: 18605822 reads failed due to low quality: 1226848 reads failed due to too many N: 840 reads failed due to too short: 106854 reads with adapter trimmed: 6476949 bases trimmed due to adapters: 117106357 reads with polyX in 3' end: 132480 bases trimmed in polyX tail: 2464673 Duplication rate: 24.1705% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L002.fastp-trim.report.html fastp --in1 lib_212401_sample_1703678_S100_L002_R1_001.fastq.gz --in2 lib_212401_sample_1703678_S100_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212401_sample_1703678_S100_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 23 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 9906870 total bases: 1000593870 Q20 bases: 978752947(97.8172%) Q30 bases: 949299559(94.8736%) Read2 before filtering: total reads: 9906870 total bases: 1000593870 Q20 bases: 965502838(96.493%) Q30 bases: 933582911(93.3029%) Read1 after filtering: total reads: 9545361 total bases: 776732619 Q20 bases: 763993480(98.3599%) Q30 bases: 741781557(95.5002%) Read2 after filtering: total reads: 9545361 total bases: 776105719 Q20 bases: 762642735(98.2653%) Q30 bases: 740052985(95.3547%) Filtering result: reads passed filter: 19090722 reads failed due to low quality: 648128 reads failed due to too many N: 804 reads failed due to too short: 74086 reads with adapter trimmed: 5907886 bases trimmed due to adapters: 90637976 reads with polyX in 3' end: 113027 bases trimmed in polyX tail: 1999480 Duplication rate: 23.7294% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L001.fastp-trim.report.html fastp --in1 lib_212402_sample_1703697_S108_L001_R1_001.fastq.gz --in2 lib_212402_sample_1703697_S108_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 25 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 9886439 total bases: 998530339 Q20 bases: 976110171(97.7547%) Q30 bases: 946419918(94.7813%) Read2 before filtering: total reads: 9886439 total bases: 998530339 Q20 bases: 963268030(96.4686%) Q30 bases: 931078079(93.2448%) Read1 after filtering: total reads: 9521462 total bases: 774927543 Q20 bases: 761967436(98.3276%) Q30 bases: 739489815(95.427%) Read2 after filtering: total reads: 9521462 total bases: 774281797 Q20 bases: 760680236(98.2433%) Q30 bases: 737912017(95.3028%) Filtering result: reads passed filter: 19042924 reads failed due to low quality: 648332 reads failed due to too many N: 852 reads failed due to too short: 80770 reads with adapter trimmed: 5879668 bases trimmed due to adapters: 90125816 reads with polyX in 3' end: 115192 bases trimmed in polyX tail: 2066713 Duplication rate: 23.6346% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L002.fastp-trim.report.html fastp --in1 lib_212402_sample_1703697_S108_L002_R1_001.fastq.gz --in2 lib_212402_sample_1703697_S108_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212402_sample_1703697_S108_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 24 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 11291366 total bases: 1140427966 Q20 bases: 1103005077(96.7185%) Q30 bases: 1067946596(93.6444%) Read2 before filtering: total reads: 11291366 total bases: 1140427966 Q20 bases: 1065123181(93.3968%) Q30 bases: 1018970176(89.3498%) Read1 after filtering: total reads: 10373068 total bases: 838758504 Q20 bases: 824627807(98.3153%) Q30 bases: 800487881(95.4372%) Read2 after filtering: total reads: 10373068 total bases: 838017496 Q20 bases: 821043089(97.9745%) Q30 bases: 793838467(94.7281%) Filtering result: reads passed filter: 20746136 reads failed due to low quality: 1716030 reads failed due to too many N: 916 reads failed due to too short: 119650 reads with adapter trimmed: 6000642 bases trimmed due to adapters: 110247214 reads with polyX in 3' end: 169573 bases trimmed in polyX tail: 3281221 Duplication rate: 20.1547% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L001.fastp-trim.report.html fastp --in1 lib_212403_sample_1703752_S117_L001_R1_001.fastq.gz --in2 lib_212403_sample_1703752_S117_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 24 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 11267830 total bases: 1138050830 Q20 bases: 1100237656(96.6774%) Q30 bases: 1065089831(93.589%) Read2 before filtering: total reads: 11267830 total bases: 1138050830 Q20 bases: 1063019003(93.407%) Q30 bases: 1016560983(89.3247%) Read1 after filtering: total reads: 10348479 total bases: 836888822 Q20 bases: 822623388(98.2954%) Q30 bases: 798336287(95.3934%) Read2 after filtering: total reads: 10348479 total bases: 836156177 Q20 bases: 818958148(97.9432%) Q30 bases: 791530550(94.663%) Filtering result: reads passed filter: 20696958 reads failed due to low quality: 1705790 reads failed due to too many N: 980 reads failed due to too short: 131932 reads with adapter trimmed: 5966315 bases trimmed due to adapters: 109729837 reads with polyX in 3' end: 176413 bases trimmed in polyX tail: 3492647 Duplication rate: 20.1158% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L002.fastp-trim.report.html fastp --in1 lib_212403_sample_1703752_S117_L002_R1_001.fastq.gz --in2 lib_212403_sample_1703752_S117_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212403_sample_1703752_S117_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 23 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 7904342 total bases: 798338542 Q20 bases: 785441302(98.3845%) Q30 bases: 764125906(95.7145%) Read2 before filtering: total reads: 7904342 total bases: 798338542 Q20 bases: 778703656(97.5405%) Q30 bases: 755594675(94.6459%) Read1 after filtering: total reads: 7711911 total bases: 623014018 Q20 bases: 614444020(98.6244%) Q30 bases: 597916981(95.9717%) Read2 after filtering: total reads: 7711911 total bases: 622554628 Q20 bases: 614283643(98.6714%) Q30 bases: 597607538(95.9928%) Filtering result: reads passed filter: 15423822 reads failed due to low quality: 294132 reads failed due to too many N: 660 reads failed due to too short: 90070 reads with adapter trimmed: 5186655 bases trimmed due to adapters: 81619255 reads with polyX in 3' end: 162386 bases trimmed in polyX tail: 2390085 Duplication rate: 25.5287% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L001.fastp-trim.report.html fastp --in1 lib_212404_sample_1703681_S101_L001_R1_001.fastq.gz --in2 lib_212404_sample_1703681_S101_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 16 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 7887897 total bases: 796677597 Q20 bases: 783323854(98.3238%) Q30 bases: 761752203(95.6161%) Read2 before filtering: total reads: 7887897 total bases: 796677597 Q20 bases: 776961082(97.5252%) Q30 bases: 753697602(94.6051%) Read1 after filtering: total reads: 7691939 total bases: 621531902 Q20 bases: 612748546(98.5868%) Q30 bases: 595986324(95.8899%) Read2 after filtering: total reads: 7691939 total bases: 621075815 Q20 bases: 612709220(98.6529%) Q30 bases: 595941672(95.9531%) Filtering result: reads passed filter: 15383878 reads failed due to low quality: 286382 reads failed due to too many N: 664 reads failed due to too short: 104870 reads with adapter trimmed: 5154436 bases trimmed due to adapters: 81190804 reads with polyX in 3' end: 163782 bases trimmed in polyX tail: 2436969 Duplication rate: 25.4001% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L002.fastp-trim.report.html fastp --in1 lib_212404_sample_1703681_S101_L002_R1_001.fastq.gz --in2 lib_212404_sample_1703681_S101_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212404_sample_1703681_S101_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 16 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 8229686 total bases: 831198286 Q20 bases: 816830276(98.2714%) Q30 bases: 794543281(95.5901%) Read2 before filtering: total reads: 8229686 total bases: 831198286 Q20 bases: 808938499(97.322%) Q30 bases: 784725750(94.409%) Read1 after filtering: total reads: 7997748 total bases: 646812264 Q20 bases: 637855623(98.6153%) Q30 bases: 620797344(95.978%) Read2 after filtering: total reads: 7997748 total bases: 646278969 Q20 bases: 638125476(98.7384%) Q30 bases: 621318257(96.1378%) Filtering result: reads passed filter: 15995496 reads failed due to low quality: 404172 reads failed due to too many N: 638 reads failed due to too short: 59066 reads with adapter trimmed: 5301263 bases trimmed due to adapters: 83122097 reads with polyX in 3' end: 169892 bases trimmed in polyX tail: 2424437 Duplication rate: 25.0671% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L001.fastp-trim.report.html fastp --in1 lib_212405_sample_1703708_S109_L001_R1_001.fastq.gz --in2 lib_212405_sample_1703708_S109_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 18 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 8200566 total bases: 828257166 Q20 bases: 813388903(98.2049%) Q30 bases: 790952736(95.496%) Read2 before filtering: total reads: 8200566 total bases: 828257166 Q20 bases: 805945197(97.3062%) Q30 bases: 781604977(94.3674%) Read1 after filtering: total reads: 7965535 total bases: 644478779 Q20 bases: 635305224(98.5766%) Q30 bases: 618054870(95.9%) Read2 after filtering: total reads: 7965535 total bases: 643953274 Q20 bases: 635741256(98.7247%) Q30 bases: 618858412(96.103%) Filtering result: reads passed filter: 15931070 reads failed due to low quality: 402454 reads failed due to too many N: 754 reads failed due to too short: 66854 reads with adapter trimmed: 5251587 bases trimmed due to adapters: 82224969 reads with polyX in 3' end: 173031 bases trimmed in polyX tail: 2491993 Duplication rate: 24.975% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L002.fastp-trim.report.html fastp --in1 lib_212405_sample_1703708_S109_L002_R1_001.fastq.gz --in2 lib_212405_sample_1703708_S109_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212405_sample_1703708_S109_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 18 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 11800392 total bases: 1191839592 Q20 bases: 1162384503(97.5286%) Q30 bases: 1127573561(94.6078%) Read2 before filtering: total reads: 11800392 total bases: 1191839592 Q20 bases: 1133641748(95.117%) Q30 bases: 1089693333(91.4295%) Read1 after filtering: total reads: 11163585 total bases: 911847709 Q20 bases: 897185835(98.3921%) Q30 bases: 871711339(95.5983%) Read2 after filtering: total reads: 11163585 total bases: 911038024 Q20 bases: 893471061(98.0718%) Q30 bases: 864385504(94.8792%) Filtering result: reads passed filter: 22327170 reads failed due to low quality: 1185328 reads failed due to too many N: 992 reads failed due to too short: 87294 reads with adapter trimmed: 6431309 bases trimmed due to adapters: 98678156 reads with polyX in 3' end: 150633 bases trimmed in polyX tail: 3029845 Duplication rate: 21.8963% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L001.fastp-trim.report.html fastp --in1 lib_212406_sample_1703759_S118_L001_R1_001.fastq.gz --in2 lib_212406_sample_1703759_S118_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 28 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 11772690 total bases: 1189041690 Q20 bases: 1158919983(97.4667%) Q30 bases: 1124099181(94.5382%) Read2 before filtering: total reads: 11772690 total bases: 1189041690 Q20 bases: 1130765518(95.0989%) Q30 bases: 1086515625(91.3774%) Read1 after filtering: total reads: 11129955 total bases: 909228568 Q20 bases: 894430136(98.3724%) Q30 bases: 868811721(95.5548%) Read2 after filtering: total reads: 11129955 total bases: 908394343 Q20 bases: 890702770(98.0524%) Q30 bases: 861458954(94.8331%) Filtering result: reads passed filter: 22259910 reads failed due to low quality: 1192462 reads failed due to too many N: 972 reads failed due to too short: 92036 reads with adapter trimmed: 6401907 bases trimmed due to adapters: 98131985 reads with polyX in 3' end: 152449 bases trimmed in polyX tail: 3100798 Duplication rate: 21.8082% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L002.fastp-trim.report.html fastp --in1 lib_212406_sample_1703759_S118_L002_R1_001.fastq.gz --in2 lib_212406_sample_1703759_S118_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212406_sample_1703759_S118_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 28 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 9125392 total bases: 921664592 Q20 bases: 902052678(97.8721%) Q30 bases: 874868280(94.9226%) Read2 before filtering: total reads: 9125392 total bases: 921664592 Q20 bases: 882577208(95.759%) Q30 bases: 851495659(92.3867%) Read1 after filtering: total reads: 8708180 total bases: 704516676 Q20 bases: 692993706(98.3644%) Q30 bases: 672964363(95.5214%) Read2 after filtering: total reads: 8708180 total bases: 703976152 Q20 bases: 691442505(98.2196%) Q30 bases: 670488461(95.2431%) Filtering result: reads passed filter: 17416360 reads failed due to low quality: 766256 reads failed due to too many N: 746 reads failed due to too short: 67422 reads with adapter trimmed: 5621266 bases trimmed due to adapters: 91401298 reads with polyX in 3' end: 104396 bases trimmed in polyX tail: 1924262 Duplication rate: 23.0365% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L001.fastp-trim.report.html fastp --in1 lib_212407_sample_1703682_S102_L001_R1_001.fastq.gz --in2 lib_212407_sample_1703682_S102_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 23 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 9108127 total bases: 919920827 Q20 bases: 899754901(97.8079%) Q30 bases: 872217887(94.8145%) Read2 before filtering: total reads: 9108127 total bases: 919920827 Q20 bases: 880822735(95.7498%) Q30 bases: 849456373(92.3402%) Read1 after filtering: total reads: 8688504 total bases: 703058631 Q20 bases: 691276692(98.3242%) Q30 bases: 670938143(95.4313%) Read2 after filtering: total reads: 8688504 total bases: 702525566 Q20 bases: 689862395(98.1975%) Q30 bases: 668764495(95.1943%) Filtering result: reads passed filter: 17377008 reads failed due to low quality: 767980 reads failed due to too many N: 880 reads failed due to too short: 70386 reads with adapter trimmed: 5586502 bases trimmed due to adapters: 90900185 reads with polyX in 3' end: 104703 bases trimmed in polyX tail: 1948888 Duplication rate: 22.9816% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L002.fastp-trim.report.html fastp --in1 lib_212407_sample_1703682_S102_L002_R1_001.fastq.gz --in2 lib_212407_sample_1703682_S102_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212407_sample_1703682_S102_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 22 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 9094247 total bases: 918518947 Q20 bases: 896320875(97.5833%) Q30 bases: 867912235(94.4904%) Read2 before filtering: total reads: 9094247 total bases: 918518947 Q20 bases: 875709319(95.3393%) Q30 bases: 843217674(91.8019%) Read1 after filtering: total reads: 8613300 total bases: 689215008 Q20 bases: 677794595(98.343%) Q30 bases: 657856151(95.4501%) Read2 after filtering: total reads: 8613300 total bases: 688739503 Q20 bases: 675865197(98.1307%) Q30 bases: 654546194(95.0354%) Filtering result: reads passed filter: 17226600 reads failed due to low quality: 868490 reads failed due to too many N: 746 reads failed due to too short: 92658 reads with adapter trimmed: 5838719 bases trimmed due to adapters: 107319796 reads with polyX in 3' end: 101852 bases trimmed in polyX tail: 1748989 Duplication rate: 21.4034% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L001.fastp-trim.report.html fastp --in1 lib_212408_sample_1703712_S110_L001_R1_001.fastq.gz --in2 lib_212408_sample_1703712_S110_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 22 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 9084462 total bases: 917530662 Q20 bases: 894492567(97.4891%) Q30 bases: 865859831(94.3685%) Read2 before filtering: total reads: 9084462 total bases: 917530662 Q20 bases: 874610660(95.3222%) Q30 bases: 841810570(91.7474%) Read1 after filtering: total reads: 8600318 total bases: 688343194 Q20 bases: 676672485(98.3045%) Q30 bases: 656428194(95.3635%) Read2 after filtering: total reads: 8600318 total bases: 687837358 Q20 bases: 674842228(98.1107%) Q30 bases: 653353821(94.9867%) Filtering result: reads passed filter: 17200636 reads failed due to low quality: 872350 reads failed due to too many N: 782 reads failed due to too short: 95156 reads with adapter trimmed: 5821072 bases trimmed due to adapters: 106851919 reads with polyX in 3' end: 103001 bases trimmed in polyX tail: 1800787 Duplication rate: 21.3153% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L002.fastp-trim.report.html fastp --in1 lib_212408_sample_1703712_S110_L002_R1_001.fastq.gz --in2 lib_212408_sample_1703712_S110_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212408_sample_1703712_S110_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 21 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10561106 total bases: 1066671706 Q20 bases: 1034435573(96.9779%) Q30 bases: 1002611202(93.9944%) Read2 before filtering: total reads: 10561106 total bases: 1066671706 Q20 bases: 1006401716(94.3497%) Q30 bases: 966515993(90.6104%) Read1 after filtering: total reads: 9830891 total bases: 801223012 Q20 bases: 787784792(98.3228%) Q30 bases: 764920308(95.4691%) Read2 after filtering: total reads: 9830891 total bases: 800518149 Q20 bases: 785506030(98.1247%) Q30 bases: 760941046(95.0561%) Filtering result: reads passed filter: 19661782 reads failed due to low quality: 1329336 reads failed due to too many N: 840 reads failed due to too short: 130254 reads with adapter trimmed: 5457308 bases trimmed due to adapters: 90937911 reads with polyX in 3' end: 165490 bases trimmed in polyX tail: 3274193 Duplication rate: 19.4433% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L001.fastp-trim.report.html fastp --in1 lib_212409_sample_1703780_S119_L001_R1_001.fastq.gz --in2 lib_212409_sample_1703780_S119_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 23 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10533407 total bases: 1063874107 Q20 bases: 1031593332(96.9657%) Q30 bases: 999570056(93.9557%) Read2 before filtering: total reads: 10533407 total bases: 1063874107 Q20 bases: 1003842093(94.3572%) Q30 bases: 963723743(90.5863%) Read1 after filtering: total reads: 9802162 total bases: 798898694 Q20 bases: 785346569(98.3036%) Q30 bases: 762358756(95.4262%) Read2 after filtering: total reads: 9802162 total bases: 798151707 Q20 bases: 782976998(98.0988%) Q30 bases: 758248062(95.0005%) Filtering result: reads passed filter: 19604324 reads failed due to low quality: 1310008 reads failed due to too many N: 924 reads failed due to too short: 151558 reads with adapter trimmed: 5428750 bases trimmed due to adapters: 90588127 reads with polyX in 3' end: 172347 bases trimmed in polyX tail: 3491473 Duplication rate: 19.4261% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L002.fastp-trim.report.html fastp --in1 lib_212409_sample_1703780_S119_L002_R1_001.fastq.gz --in2 lib_212409_sample_1703780_S119_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212409_sample_1703780_S119_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 23 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10232495 total bases: 1033481995 Q20 bases: 1009154260(97.646%) Q30 bases: 977614032(94.5942%) Read2 before filtering: total reads: 10232495 total bases: 1033481995 Q20 bases: 989305666(95.7255%) Q30 bases: 952141198(92.1294%) Read1 after filtering: total reads: 9772482 total bases: 786121896 Q20 bases: 772914947(98.32%) Q30 bases: 749982871(95.4029%) Read2 after filtering: total reads: 9772482 total bases: 785535621 Q20 bases: 770366491(98.0689%) Q30 bases: 745188938(94.8638%) Filtering result: reads passed filter: 19544964 reads failed due to low quality: 841348 reads failed due to too many N: 832 reads failed due to too short: 77846 reads with adapter trimmed: 6640913 bases trimmed due to adapters: 112110079 reads with polyX in 3' end: 117382 bases trimmed in polyX tail: 2091031 Duplication rate: 22.5447% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L001.fastp-trim.report.html fastp --in1 lib_212410_sample_1703683_S103_L001_R1_001.fastq.gz --in2 lib_212410_sample_1703683_S103_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 24 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10217470 total bases: 1031964470 Q20 bases: 1006849606(97.5663%) Q30 bases: 975050178(94.4849%) Read2 before filtering: total reads: 10217470 total bases: 1031964470 Q20 bases: 987578345(95.6989%) Q30 bases: 950057359(92.063%) Read1 after filtering: total reads: 9752063 total bases: 784603194 Q20 bases: 771158669(98.2865%) Q30 bases: 747936658(95.3267%) Read2 after filtering: total reads: 9752063 total bases: 784022372 Q20 bases: 768661378(98.0407%) Q30 bases: 743240921(94.7984%) Filtering result: reads passed filter: 19504126 reads failed due to low quality: 849610 reads failed due to too many N: 868 reads failed due to too short: 80336 reads with adapter trimmed: 6610031 bases trimmed due to adapters: 111721421 reads with polyX in 3' end: 118177 bases trimmed in polyX tail: 2133382 Duplication rate: 22.4632% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L002.fastp-trim.report.html fastp --in1 lib_212410_sample_1703683_S103_L002_R1_001.fastq.gz --in2 lib_212410_sample_1703683_S103_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212410_sample_1703683_S103_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 24 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10145533 total bases: 1024698833 Q20 bases: 999594693(97.5501%) Q30 bases: 968146353(94.4811%) Read2 before filtering: total reads: 10145533 total bases: 1024698833 Q20 bases: 968384768(94.5043%) Q30 bases: 931881449(90.942%) Read1 after filtering: total reads: 9489154 total bases: 766037922 Q20 bases: 753624739(98.3796%) Q30 bases: 732229639(95.5866%) Read2 after filtering: total reads: 9489154 total bases: 765415910 Q20 bases: 752240029(98.2786%) Q30 bases: 730519646(95.4409%) Filtering result: reads passed filter: 18978308 reads failed due to low quality: 1224976 reads failed due to too many N: 882 reads failed due to too short: 86900 reads with adapter trimmed: 6103126 bases trimmed due to adapters: 103469392 reads with polyX in 3' end: 135388 bases trimmed in polyX tail: 2495834 Duplication rate: 22.8043% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L001.fastp-trim.report.html fastp --in1 lib_212411_sample_1703714_S111_L001_R1_001.fastq.gz --in2 lib_212411_sample_1703714_S111_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 24 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10121538 total bases: 1022275338 Q20 bases: 996124320(97.4419%) Q30 bases: 964408351(94.3394%) Read2 before filtering: total reads: 10121538 total bases: 1022275338 Q20 bases: 966017524(94.4968%) Q30 bases: 929177227(90.893%) Read1 after filtering: total reads: 9462591 total bases: 764042115 Q20 bases: 751344964(98.3382%) Q30 bases: 729607258(95.4931%) Read2 after filtering: total reads: 9462591 total bases: 763407182 Q20 bases: 750049330(98.2502%) Q30 bases: 728147807(95.3813%) Filtering result: reads passed filter: 18925182 reads failed due to low quality: 1228864 reads failed due to too many N: 762 reads failed due to too short: 88268 reads with adapter trimmed: 6074111 bases trimmed due to adapters: 102925638 reads with polyX in 3' end: 130976 bases trimmed in polyX tail: 2470895 Duplication rate: 22.6934% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L002.fastp-trim.report.html fastp --in1 lib_212411_sample_1703714_S111_L002_R1_001.fastq.gz --in2 lib_212411_sample_1703714_S111_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212411_sample_1703714_S111_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 24 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 11581466 total bases: 1169728066 Q20 bases: 1142344146(97.6589%) Q30 bases: 1110086038(94.9012%) Read2 before filtering: total reads: 11581466 total bases: 1169728066 Q20 bases: 1117237798(95.5126%) Q30 bases: 1077103947(92.0816%) Read1 after filtering: total reads: 10947330 total bases: 888503361 Q20 bases: 876269792(98.6231%) Q30 bases: 852856872(95.988%) Read2 after filtering: total reads: 10947330 total bases: 887778077 Q20 bases: 875827940(98.6539%) Q30 bases: 851398084(95.9021%) Filtering result: reads passed filter: 21894660 reads failed due to low quality: 1168814 reads failed due to too many N: 890 reads failed due to too short: 98568 reads with adapter trimmed: 6025734 bases trimmed due to adapters: 108681301 reads with polyX in 3' end: 257108 bases trimmed in polyX tail: 3862670 Duplication rate: 25.0265% Insert size peak (evaluated by paired-end reads): 117 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L001.fastp-trim.report.html fastp --in1 lib_212412_sample_1703783_S120_L001_R1_001.fastq.gz --in2 lib_212412_sample_1703783_S120_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 22 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 11573464 total bases: 1168919864 Q20 bases: 1140693820(97.5853%) Q30 bases: 1108315571(94.8154%) Read2 before filtering: total reads: 11573464 total bases: 1168919864 Q20 bases: 1116730558(95.5353%) Q30 bases: 1076263125(92.0733%) Read1 after filtering: total reads: 10938434 total bases: 888076956 Q20 bases: 875556676(98.5902%) Q30 bases: 851824034(95.9178%) Read2 after filtering: total reads: 10938434 total bases: 887347323 Q20 bases: 875259653(98.6378%) Q30 bases: 850618553(95.8608%) Filtering result: reads passed filter: 21876868 reads failed due to low quality: 1168612 reads failed due to too many N: 990 reads failed due to too short: 100458 reads with adapter trimmed: 5990740 bases trimmed due to adapters: 107947338 reads with polyX in 3' end: 256355 bases trimmed in polyX tail: 3881005 Duplication rate: 24.9486% Insert size peak (evaluated by paired-end reads): 117 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L002.fastp-trim.report.html fastp --in1 lib_212412_sample_1703783_S120_L002_R1_001.fastq.gz --in2 lib_212412_sample_1703783_S120_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212412_sample_1703783_S120_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 23 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 8358319 total bases: 844190219 Q20 bases: 829172560(98.2211%) Q30 bases: 807441410(95.6469%) Read2 before filtering: total reads: 8358319 total bases: 844190219 Q20 bases: 822053756(97.3778%) Q30 bases: 797316516(94.4475%) Read1 after filtering: total reads: 8140126 total bases: 654899324 Q20 bases: 646201815(98.6719%) Q30 bases: 629544013(96.1284%) Read2 after filtering: total reads: 8140126 total bases: 654405041 Q20 bases: 646253277(98.7543%) Q30 bases: 629363884(96.1734%) Filtering result: reads passed filter: 16280252 reads failed due to low quality: 380372 reads failed due to too many N: 720 reads failed due to too short: 55294 reads with adapter trimmed: 5649423 bases trimmed due to adapters: 91756629 reads with polyX in 3' end: 169181 bases trimmed in polyX tail: 2430096 Duplication rate: 26.5813% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L001.fastp-trim.report.html fastp --in1 lib_212413_sample_1703687_S104_L001_R1_001.fastq.gz --in2 lib_212413_sample_1703687_S104_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 17 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 8328633 total bases: 841191933 Q20 bases: 825643719(98.1516%) Q30 bases: 803745541(95.5484%) Read2 before filtering: total reads: 8328633 total bases: 841191933 Q20 bases: 818993083(97.361%) Q30 bases: 794135344(94.406%) Read1 after filtering: total reads: 8107640 total bases: 652496797 Q20 bases: 643558366(98.6301%) Q30 bases: 626661935(96.0406%) Read2 after filtering: total reads: 8107640 total bases: 651993097 Q20 bases: 643775063(98.7396%) Q30 bases: 626817706(96.1387%) Filtering result: reads passed filter: 16215280 reads failed due to low quality: 385336 reads failed due to too many N: 776 reads failed due to too short: 55874 reads with adapter trimmed: 5604185 bases trimmed due to adapters: 90990193 reads with polyX in 3' end: 167888 bases trimmed in polyX tail: 2411900 Duplication rate: 26.4045% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L002.fastp-trim.report.html fastp --in1 lib_212413_sample_1703687_S104_L002_R1_001.fastq.gz --in2 lib_212413_sample_1703687_S104_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212413_sample_1703687_S104_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 18 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10514200 total bases: 1061934200 Q20 bases: 1045189455(98.4232%) Q30 bases: 1017975391(95.8605%) Read2 before filtering: total reads: 10514200 total bases: 1061934200 Q20 bases: 1035673205(97.5271%) Q30 bases: 1004143906(94.558%) Read1 after filtering: total reads: 10266463 total bases: 832816900 Q20 bases: 821323358(98.6199%) Q30 bases: 800044209(96.0648%) Read2 after filtering: total reads: 10266463 total bases: 832195951 Q20 bases: 821116153(98.6686%) Q30 bases: 798570764(95.9595%) Filtering result: reads passed filter: 20532926 reads failed due to low quality: 419290 reads failed due to too many N: 848 reads failed due to too short: 75336 reads with adapter trimmed: 6714458 bases trimmed due to adapters: 101308407 reads with polyX in 3' end: 214576 bases trimmed in polyX tail: 3106472 Duplication rate: 29.9906% Insert size peak (evaluated by paired-end reads): 117 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L001.fastp-trim.report.html fastp --in1 lib_212414_sample_1703716_S112_L001_R1_001.fastq.gz --in2 lib_212414_sample_1703716_S112_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 21 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10501548 total bases: 1060656348 Q20 bases: 1043376911(98.3709%) Q30 bases: 1015795542(95.7705%) Read2 before filtering: total reads: 10501548 total bases: 1060656348 Q20 bases: 1034382131(97.5228%) Q30 bases: 1002629782(94.5292%) Read1 after filtering: total reads: 10250840 total bases: 831948122 Q20 bases: 820166179(98.5838%) Q30 bases: 798548944(95.9854%) Read2 after filtering: total reads: 10250840 total bases: 831316588 Q20 bases: 820130570(98.6544%) Q30 bases: 797426847(95.9234%) Filtering result: reads passed filter: 20501680 reads failed due to low quality: 418328 reads failed due to too many N: 954 reads failed due to too short: 82134 reads with adapter trimmed: 6669498 bases trimmed due to adapters: 100400936 reads with polyX in 3' end: 215481 bases trimmed in polyX tail: 3159989 Duplication rate: 29.9193% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L002.fastp-trim.report.html fastp --in1 lib_212414_sample_1703716_S112_L002_R1_001.fastq.gz --in2 lib_212414_sample_1703716_S112_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212414_sample_1703716_S112_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 21 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 11357936 total bases: 1147151536 Q20 bases: 1125889175(98.1465%) Q30 bases: 1096069265(95.547%) Read2 before filtering: total reads: 11357936 total bases: 1147151536 Q20 bases: 1113937770(97.1047%) Q30 bases: 1080479583(94.188%) Read1 after filtering: total reads: 11013253 total bases: 896912525 Q20 bases: 884607120(98.628%) Q30 bases: 861416031(96.0424%) Read2 after filtering: total reads: 11013253 total bases: 896201226 Q20 bases: 884399534(98.6831%) Q30 bases: 860350434(95.9997%) Filtering result: reads passed filter: 22026506 reads failed due to low quality: 615034 reads failed due to too many N: 996 reads failed due to too short: 73336 reads with adapter trimmed: 6528039 bases trimmed due to adapters: 101464266 reads with polyX in 3' end: 243733 bases trimmed in polyX tail: 3763037 Duplication rate: 25.8785% Insert size peak (evaluated by paired-end reads): 117 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L001.fastp-trim.report.html fastp --in1 lib_212415_sample_1703795_S121_L001_R1_001.fastq.gz --in2 lib_212415_sample_1703795_S121_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 22 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 11348838 total bases: 1146232638 Q20 bases: 1124387791(98.0942%) Q30 bases: 1094307908(95.47%) Read2 before filtering: total reads: 11348838 total bases: 1146232638 Q20 bases: 1113051407(97.1052%) Q30 bases: 1079263666(94.1575%) Read1 after filtering: total reads: 11000994 total bases: 896190722 Q20 bases: 883614605(98.5967%) Q30 bases: 860092421(95.972%) Read2 after filtering: total reads: 11000994 total bases: 895448892 Q20 bases: 883527763(98.6687%) Q30 bases: 859268969(95.9596%) Filtering result: reads passed filter: 22001988 reads failed due to low quality: 616388 reads failed due to too many N: 1042 reads failed due to too short: 78258 reads with adapter trimmed: 6488517 bases trimmed due to adapters: 100864994 reads with polyX in 3' end: 246487 bases trimmed in polyX tail: 3850440 Duplication rate: 25.8178% Insert size peak (evaluated by paired-end reads): 117 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L002.fastp-trim.report.html fastp --in1 lib_212415_sample_1703795_S121_L002_R1_001.fastq.gz --in2 lib_212415_sample_1703795_S121_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212415_sample_1703795_S121_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 22 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10456168 total bases: 1056072968 Q20 bases: 1033676875(97.8793%) Q30 bases: 1002152849(94.8943%) Read2 before filtering: total reads: 10456168 total bases: 1056072968 Q20 bases: 1017649960(96.3617%) Q30 bases: 980926181(92.8843%) Read1 after filtering: total reads: 10065812 total bases: 817990584 Q20 bases: 804998782(98.4117%) Q30 bases: 781250680(95.5085%) Read2 after filtering: total reads: 10065812 total bases: 817345161 Q20 bases: 802605703(98.1967%) Q30 bases: 776925394(95.0547%) Filtering result: reads passed filter: 20131624 reads failed due to low quality: 653676 reads failed due to too many N: 834 reads failed due to too short: 126202 reads with adapter trimmed: 6412754 bases trimmed due to adapters: 97661413 reads with polyX in 3' end: 123371 bases trimmed in polyX tail: 2346031 Duplication rate: 24.1434% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L001.fastp-trim.report.html fastp --in1 lib_212416_sample_1703688_S105_L001_R1_001.fastq.gz --in2 lib_212416_sample_1703688_S105_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 24 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10440609 total bases: 1054501509 Q20 bases: 1031370024(97.8064%) Q30 bases: 999529698(94.7869%) Read2 before filtering: total reads: 10440609 total bases: 1054501509 Q20 bases: 1015917002(96.341%) Q30 bases: 978825276(92.8235%) Read1 after filtering: total reads: 10045940 total bases: 816468757 Q20 bases: 803177065(98.3721%) Q30 bases: 779079232(95.4206%) Read2 after filtering: total reads: 10045940 total bases: 815828592 Q20 bases: 800860173(98.1652%) Q30 bases: 774948904(94.9892%) Filtering result: reads passed filter: 20091880 reads failed due to low quality: 642726 reads failed due to too many N: 910 reads failed due to too short: 145702 reads with adapter trimmed: 6392750 bases trimmed due to adapters: 97303674 reads with polyX in 3' end: 124781 bases trimmed in polyX tail: 2414312 Duplication rate: 24.0427% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L002.fastp-trim.report.html fastp --in1 lib_212416_sample_1703688_S105_L002_R1_001.fastq.gz --in2 lib_212416_sample_1703688_S105_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212416_sample_1703688_S105_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 24 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10424922 total bases: 1052917122 Q20 bases: 1030153812(97.8381%) Q30 bases: 1000768753(95.0472%) Read2 before filtering: total reads: 10424922 total bases: 1052917122 Q20 bases: 1016580816(96.549%) Q30 bases: 981713899(93.2375%) Read1 after filtering: total reads: 10080642 total bases: 815996138 Q20 bases: 803350847(98.4503%) Q30 bases: 780920875(95.7015%) Read2 after filtering: total reads: 10080642 total bases: 815365581 Q20 bases: 801075945(98.2475%) Q30 bases: 776549442(95.2394%) Filtering result: reads passed filter: 20161284 reads failed due to low quality: 623284 reads failed due to too many N: 862 reads failed due to too short: 64414 reads with adapter trimmed: 6853123 bases trimmed due to adapters: 104116343 reads with polyX in 3' end: 110162 bases trimmed in polyX tail: 1958631 Duplication rate: 23.0799% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L001.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L001.fastp-trim.report.html fastp --in1 lib_212417_sample_1703718_S113_L001_R1_001.fastq.gz --in2 lib_212417_sample_1703718_S113_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L001_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 25 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT WARNING: fastp uses up to 16 threads although you specified 50 Read1 before filtering: total reads: 10398379 total bases: 1050236279 Q20 bases: 1027080918(97.7952%) Q30 bases: 997447812(94.9737%) Read2 before filtering: total reads: 10398379 total bases: 1050236279 Q20 bases: 1013705521(96.5217%) Q30 bases: 978535506(93.1729%) Read1 after filtering: total reads: 10050829 total bases: 813598381 Q20 bases: 800735855(98.4191%) Q30 bases: 778051615(95.6309%) Read2 after filtering: total reads: 10050829 total bases: 812942236 Q20 bases: 798484097(98.2215%) Q30 bases: 773753558(95.1794%) Filtering result: reads passed filter: 20101658 reads failed due to low quality: 629022 reads failed due to too many N: 924 reads failed due to too short: 65154 reads with adapter trimmed: 6824467 bases trimmed due to adapters: 103839773 reads with polyX in 3' end: 110279 bases trimmed in polyX tail: 1970660 Duplication rate: 22.9754% Insert size peak (evaluated by paired-end reads): 116 JSON report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L002.fastp-trim.report.json HTML report: /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L002.fastp-trim.report.html fastp --in1 lib_212417_sample_1703718_S113_L002_R1_001.fastq.gz --in2 lib_212417_sample_1703718_S113_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260520-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_212417_sample_1703718_S113_L002_R2_001.fastp-trim.fq.gz fastp v0.23.4, time used: 24 seconds