Version   MEGAN Community Edition (version 6.24.20, built 5 Feb 2023)
Author(s) Daniel H. Huson
Copyright (C) 2023 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY.
Functional classifications to use: EC, EGGNOG, GTDB, INTERPRO2GO, SEED
Loading ncbi.map: 2,396,736
Loading ncbi.tre: 2,396,740
Loading ec.map:     8,200
Loading ec.tre:     8,204
Loading eggnog.map:    30,875
Loading eggnog.tre:    30,986
Loading gtdb.map:   240,103
Loading gtdb.tre:   240,107
Loading interpro2go.map:    14,242
Loading interpro2go.tre:    28,907
Loading seed.map:       961
Loading seed.tre:       962
In DAA file:  ./PSC-0519_unmapped_reads.1.blastx.meganized.daa
Output file:  ./PSC-0519_unmapped_reads.1.blastx.meganized.rma6
Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO
Generating RMA6 file Parsing matches
Annotating RMA6 file using FAST mode (accession database and first accession per line)
Parsing file PSC-0519_unmapped_reads.1.blastx.meganized.daa
Parsing file: ./PSC-0519_unmapped_reads.1.blastx.meganized.daa
10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (2,391.1s)
Total reads:         3,198,101
Alignments:         52,160,038
100% (0.0s)
Binning reads: Initializing...
Initializing binning...
Using 'Naive LCA' algorithm for binning: Taxonomy
Using Best-Hit algorithm for binning: SEED
Using Best-Hit algorithm for binning: EGGNOG
Using 'Naive LCA' algorithm for binning: GTDB
Using Best-Hit algorithm for binning: EC
Using Best-Hit algorithm for binning: INTERPRO2GO
Binning reads...
Binning reads: Analyzing alignments
Total reads:        3,198,101
With hits:           3,198,101 
Alignments:         52,160,038
Assig. Taxonomy:     3,053,052
Assig. SEED:            73,902
Assig. EGGNOG:           5,310
Assig. GTDB:           125,208
Assig. EC:             235,605
Assig. INTERPRO2GO:    809,747
MinSupport set to: 319
Binning reads: Applying min-support & disabled filter to Taxonomy...
Min-supp. changes:       1,951
Binning reads: Applying min-support & disabled filter to GTDB...
Min-supp. changes:         832
Binning reads: Writing classification tables
Numb. Tax. classes:        308
Numb. SEED classes:        214
Numb. EGG. classes:        295
Numb. GTDB classes:         58
Numb.   EC classes:      1,115
Numb. INT. classes:      4,204
Binning reads: Syncing
Class. Taxonomy:           308
Class. SEED:               214
Class. EGGNOG:             295
Class. GTDB:                58
Class. EC:               1,115
Class. INTERPRO2GO:      4,204
100% (1,311.6s)
In DAA file:  ./PSC-0519_unmapped_reads.2.blastx.meganized.daa
Output file:  ./PSC-0519_unmapped_reads.2.blastx.meganized.rma6
Classifications: Taxonomy, SEED, EGGNOG, GTDB, EC, INTERPRO2GO
Generating RMA6 file Parsing matches
Annotating RMA6 file using FAST mode (accession database and first accession per line)
Parsing file PSC-0519_unmapped_reads.2.blastx.meganized.daa
Parsing file: ./PSC-0519_unmapped_reads.2.blastx.meganized.daa
10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (1,502.5s)
Total reads:         3,048,532
Alignments:         49,997,819
100% (0.0s)
Binning reads: Initializing...
Initializing binning...
Using 'Naive LCA' algorithm for binning: Taxonomy
Using Best-Hit algorithm for binning: SEED
Using Best-Hit algorithm for binning: EGGNOG
Using 'Naive LCA' algorithm for binning: GTDB
Using Best-Hit algorithm for binning: EC
Using Best-Hit algorithm for binning: INTERPRO2GO
Binning reads...
Binning reads: Analyzing alignments
Total reads:        3,048,532
With hits:           3,048,532 
Alignments:         49,997,819
Assig. Taxonomy:     2,894,357
Assig. SEED:            73,284
Assig. EGGNOG:           5,252
Assig. GTDB:           124,372
Assig. EC:             228,613
Assig. INTERPRO2GO:    779,663
MinSupport set to: 304
Binning reads: Applying min-support & disabled filter to Taxonomy...
Min-supp. changes:       1,995
Binning reads: Applying min-support & disabled filter to GTDB...
Min-supp. changes:         878
Binning reads: Writing classification tables
Numb. Tax. classes:        318
Numb. SEED classes:        217
Numb. EGG. classes:        280
Numb. GTDB classes:         53
Numb.   EC classes:      1,121
Numb. INT. classes:      4,291
Binning reads: Syncing
Class. Taxonomy:           318
Class. SEED:               217
Class. EGGNOG:             280
Class. GTDB:                53
Class. EC:               1,121
Class. INTERPRO2GO:      4,291
100% (1,169.7s)
Total time:  6,386.1s
Peak memory: 86.4 of 488.3G