FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POC-57-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-57-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574698
Sequences flagged as poor quality0
Sequence length18-35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAG9751665.883461647385672No Hit
TTCCACTTCAGAGCAAAGATTTTCA7106034.2872757017955925No Hit
TGCTTCAAGTCTCAGCCCGAG4726352.851545168424788No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACC3121011.8829966012050416No Hit
GGCCAGTCCGGGCCGGATCCAAC2447431.4766060895951167No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC2045201.2339289681175487No Hit
AGAATTCTACCACTGAACCACCAGTGC1842501.111634130528351No Hit
GAGAATTCTACCACTGAACCACCAGTGC1692541.0211588772235851No Hit
CGCCCCAACGACCGGCAGCAGTCG1660931.0020876398471936No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT1629190.982937969669191Illumina RNA PCR Primer (100% over 35bp)
ACCCGAGGGTCACAGCTG1477240.8912620911705299No Hit
GCTTCAAGTCTCAGCCCGAG1368800.8258370680419035No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAG1274840.7691482523542812No Hit
AACCGCTTCAAGTCTCAGCCCGAG1175270.709074759612513No Hit
TGCCCCAACGACCGGCAGCAGTCG969420.584879434907351No Hit
TGCCAGTCCGGGCCGGATCCAAC953700.5753950992048241No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCG761360.459350752574798No Hit
AGCTGCTCCCACTTATTCTACACCTC702290.42371209418114286No Hit
AGCACTGCAAGCAGTCCACCACG663800.400489951611788No Hit
CCGCTTCAAGTCTCAGCCCGAG637890.38485769092142735No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC631230.3808395181619599No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC607830.366721613871939No Hit
AGCCAGTCCGGGCCGGATCCAAC595070.3590231327291755No Hit
AGCCTTAACTCCAGAAAGTG583320.3519340141220069No Hit
AGAATTCTACCACTGAACCACAGTGC568500.34299267473832706No Hit
GAGAATTCTACCACTGAACCACAGTGC537650.32437996758673976No Hit
GGCCAATCCACGGCCGATTCCTTCC514790.31058786108802705No Hit
GCCTCGACCACGGCCAGTCCG487970.29440657078638777No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCG477190.2879026815450876No Hit
CGGTCCGACCGCGACCTCGGAA474890.28651502428581205No Hit
ACCTCGACCACGGCCAGTCCG471500.2844697381514885No Hit
GACCGCGACCTCGGAAAG470990.2841620402374752No Hit
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAG454530.27423124089500756No Hit
ATCCAACCGCTTCAAGTCTCAGCCCGAG452600.27306681545570244No Hit
GCCCCAACGACCGGCAGCAGTCG400890.24186866029172902No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC398310.24031207084436773No Hit
GGTCTCGGCGAGGAAGAACAGCTCGG389770.23515963910775328No Hit
AAACTAACCAGCCTAGACTTGA363760.21946704549307625No Hit
CGAGAATTCTACCACTGAACCACCAGTGC360980.21778979019708233No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGC353090.213029522468524No Hit
GACCCGAGGGTCACAGCTG347640.2097413780932841No Hit
ATCCAACCGCTTCAAGTCT334000.2015119672165369No Hit
GCCCGAGGGTCACAGCTG332530.20062507322908688No Hit
GGAATTCTACCACTGAACCACCAGTGC332200.200425974578843No Hit
AGTCCGGGCCGGATCCAAC324200.19559934063353673No Hit
AGCCTTAACTCCAGAAAGTGA321020.19368075364027748No Hit
TGGTCCGACCGCGACCTCGGAA314470.189728947097558No Hit
GGCCAATCCACGGCCGATTCCTTCCCGG309770.18689329965469054No Hit
CGCTTCAAGTCTCAGCCCGAGA309650.18682090014551095No Hit
GCCAGTCCGGGCCGGATCCAAC303440.18307422554546696No Hit
CGCCCCAACGACCGGCAGCAGTC276550.16685070219680626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG267580.1614388388856316No Hit
TGCTGCTCCCACTTATTCTACACCTC264470.15956248493939376No Hit
TCATGCTCTACCGACTGAGCTAGCCGGG256540.1547780840411089No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGG250820.15132704077021494No Hit
GGTCTCGGCGAGGAAGAACAGCTCGGC250260.1509891763940435No Hit
CAACCGCTTCAAGTCTCAGCCCGAG249610.15059701238598736No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC245740.14826212821494544No Hit
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAG245070.14785789762202606No Hit
CGGTCTCGGCGAGGAAGAACAGCTCGG241900.14594534392119843No Hit
AGCTTCAAGTCTCAGCCCGAG240320.14499208371700045No Hit
TGCCTTAACTCCAGAAAGTG232740.14041884805382276No Hit
TGCCAATCCACGGCCGATTCCTTCC230590.1391216901810217No Hit
TGCACTGCAAGCAGTCCACCACG230070.1388079589745768No Hit
CAGTTAAGAGCACACTCG227750.13740823513043796No Hit
GTAGACACTCAGACAGACATGCTCCTGGGAGAACC227660.13735393549855326No Hit
ACAAGTTCGGATCTACGGGT227260.13711260380128795No Hit
GTCTCGGCGAGGAAGAACAGCTCGG217720.13135684282151022No Hit
GACCGCTTCAAGTCTCAGCCCGAG216140.13040358261731225No Hit
ACCGCTTCAAGTCTCAGCCCGAG214950.12968562081794793No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCGG210360.12691633959182846No Hit
ATCCACTTCAGAGCAAAGATTTTCA210350.1269103062993968No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC207200.12500981918343249No Hit
CGGAAACGTTCGAGTTGGCTATCGGTAGTG207120.12496155284397942No Hit
TCGCTTCAAGTCTCAGCCCGAG204480.12336876364202834No Hit
AGCCTTAACTCCAGAAAG203030.12249393623944159No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGG201930.12183027407196198No Hit
GACAGGGTCGAGTTCGACTATCTTGGTC199160.12015905206839968No Hit
CCTCGACCACGGCCAGTCCG196280.11842146384808944No Hit
TGACAGGGTCGAGTTCGA196130.11833096446161494No Hit
GTCGAGTTCGACTATCTTGGTC195610.11801723325517001No Hit
ACTTCAAGTCTCAGCCCGAG193010.11644857722294549No Hit
ATCCAACCGCTTCAAGTC191970.11582111481005566No Hit
TGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAG189800.11451189035239133No Hit
GCGAGCTGCTCCCACTTATTCTACACCTC188800.11390856110922805No Hit
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAG186230.11235800495429842No Hit
GCCAATCCACGGCCGATTC184060.11104878049663408No Hit
GGTCCGACCGCGACCTCGGAA182590.11016188650918406No Hit
TCACCTCGACCACGGCCAGTCCG182580.11015585321675243No Hit
TGAGAATTCTACCACTGAACCACCAGTGC177270.1069521749355554No Hit
GTCTCGGCGAGGAAGAACAGCTCGGC174350.1051904535455186No Hit
CCACAAGTTCGGATCTACGGGT172060.10380882957867468No Hit
TGGTCTCGGCGAGGAAGAACAGCTCGG171840.10367609714517875No Hit
GGCTGCTCCCACTTATTCTACACCTC171030.10318740045821648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)