FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POC-57-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-57-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574698
Sequences flagged as poor quality0
Sequence length18-35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG9835165.933839639189806No Hit
TGAAAATCTTTGCTCTGAAGTGGAA7140454.308042294345273No Hit
CTCGGGCTGAGACTTGAAGCA4734462.8564381685868425No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCG2948291.7787895743258793No Hit
GTTGGATCCGGCCCGGACTGGCC2500911.5088721375194891No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2100581.2673413416039316No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1853891.118506050607981No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1719611.0374909998360151No Hit
CGACTGCTGCCGGTCGTTGGGGCG1658701.0007422156349395No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAAAAG1585660.9566750477142931TruSeq Adapter, Index 7 (96% over 33bp)
CAGCTGTGACCCTCGGGT1511020.9116425530045857No Hit
CTCGGGCTGAGACTTGAAGC1415000.853710879076047No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA1280060.7722976310035936No Hit
CTCGGGCTGAGACTTGAAGCGGTT1179520.7116389088959569No Hit
GTTGGATCCGGCCCGGACTGGCA952400.5746107711887118No Hit
CGACTGCTGCCGGTCGTTGGGGCA949100.572619784686273No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA775580.46793009441257993No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT708090.4272114037914899No Hit
CGTGGTGGACTGCTTGCAGTGCT669710.404055627438883No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC647020.39036608691150815No Hit
CTCGGGCTGAGACTTGAAGCGG641630.38711414229085805No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA605720.3654485891688645No Hit
GTTGGATCCGGCCCGGACTGGCT596830.3600849921971429No Hit
CACTTTCTGGAGTTAAGGCT588090.3548118946118958No Hit
GCACTGTGGTTCAGTGGTAGAATTCT571200.3446216636948679No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC545510.3291221354380031No Hit
GGAAGGAATCGGCCGTGGATTGGCC527350.3181656763821579No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC495510.29895567327983896No Hit
CGGACTGGCCGTGGTCGAGGC495490.29894360669497566No Hit
TTCCGAGGTCGCGGTCGGACCG485290.29278964841471017No Hit
CTTTCCGAGGTCGCGGTC480870.29012293315992843No Hit
CGGACTGGCCGTGGTCGAGGT472740.285217866413011No Hit
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCT461750.2785872780306465No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT454300.27409247516908No Hit
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATC409090.24681596008566792No Hit
CGACTGCTGCCGGTCGTTGGGGC405800.24483100687566073No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC403380.24337095010720558No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT398940.2406921682675606No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG365220.22034790618809466No Hit
TCAAGTCTAGGCTGGTTAGTTT364480.2199014425481538No Hit
CAGCTGTGACCCTCGGGTC360490.21749415886793233No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA349430.2108213374385464No Hit
CAGCTGTGACCCTCGGGC344600.20790725719406775No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC339180.20463721269612273No Hit
AGACTTGAAGCGGTTGGAT337130.20340038774763802No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC332900.20084830504905732No Hit
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTC331160.19979851216595318No Hit
GTTGGATCCGGCCCGGACT327370.19751189433436434No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCC325460.19635953547992246No Hit
TCACTTTCTGGAGTTAAGGCT323630.19525544296493366No Hit
TTCCGAGGTCGCGGTCGGACCA318710.1922870630885703No Hit
GTTGGATCCGGCCCGGACTGGC314800.18992804574780184No Hit
TCTCGGGCTGAGACTTGAAGCG311740.1880818582637222No Hit
GACTGCTGCCGGTCGTTGGGGCG276640.16690500182869095No Hit
GAGGTGTAGAATAAGTGGGAGCAGCA264680.15968918408045804No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGA259130.15634070678090184No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC258740.15610540837606815No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCC252060.1520751690317374No Hit
CTCGGGCTGAGACTTGAAGCGGTTG250640.15121844150644553No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT248330.14982475095473835No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCG245230.1479544303009322No Hit
CTCGGGCTGAGACTTGAAGCT239500.14449735373760655No Hit
CACTTTCTGGAGTTAAGGCA232760.140430914638686No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGC230580.13911565688859007No Hit
CGTGGTGGACTGCTTGCAGTGCA230280.1389346581156411No Hit
ACCCGTAGATCCGAACTTGT229760.1386209269091962No Hit
CGAGTGTGCTCTTAACTG229440.13842786155138392No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC224340.13535088241125118No Hit
CTCGGGCTGAGACTTGAAGCGGTC219590.13248506850622557No Hit
GGAAGGAATCGGCCGTGGATTGGCA219380.1323583693651613No Hit
CTCGGGCTGAGACTTGAAGCGGT216350.13053028175837653No Hit
CTCGGGCTGAGACTTGAAGCGA213300.1286901275667285No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG213270.12867202768943362No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTCA212310.12809283161599685No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC208600.12585448012386108No Hit
TGAAAATCTTTGCTCTGAAGTGGAT206430.12454525566619676No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA206270.12444872298729064No Hit
GACCAAGATAGTCGAACTCGACCCTGTC205060.12371869460306305No Hit
CTTTCTGGAGTTAAGGCT204470.12336273034959672No Hit
GACCAAGATAGTCGAACTCGAC201590.12162514212928645No Hit
CGGACTGGCCGTGGTCGAGG198980.1200504528046303No Hit
TCGAACTCGACCCTGTCA198380.11968845525873231No Hit
GACTTGAAGCGGTTGGAT193400.11668387562777915No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGC192290.11601418016786791No Hit
TTCCGAGGTCGCGGTCGGACC190660.11503075350151176No Hit
CTCGGGCTGAGACTTGAAGT190380.11486182131342604No Hit
GAATCGGCCGTGGATTGGC185590.11197187423867391No Hit
CGGACTGGCCGTGGTCGAGGTGA183710.11083761526152693No Hit
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCA182430.11006535383027793No Hit
GCCGAGCTGTTCTTCCTCGCCGAGAC179160.10809246720513399No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCA179060.10803213428081766No Hit
ACCCGTAGATCCGAACTTGTGG178890.10792956830947992No Hit
GAGGTGTAGAATAAGTGGGAGCAGCC174630.10535938573360432No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCA173590.10473192332071452No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCT166150.10024315375157966No Hit
TGAAAATCTTTGCTCTGAAGTGGA166090.10020695399698987No Hit
GGAAGGAATCGGCCGTGGATTGGCCCA165870.10007422156349394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)