FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POC-53-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-53-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18481608
Sequences flagged as poor quality0
Sequence length18-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAG16575998.968911146692431No Hit
TTCCACTTCAGAGCAAAGATTTTCA9511215.146310862128447No Hit
TTCCACCTCAGAGCAAAGATTTTCA5577813.0180328464925776No Hit
TGCTTCAAGTCTCAGCCCGAG4922722.663577757952663No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACC4285552.3188187954208312No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT2386271.2911592973944692Illumina RNA PCR Primer (100% over 35bp)
GGCGAGAATTCTACCACTGAACCACCAGTGC1670410.9038228708237941No Hit
GGCCAGTCCGGGCCGGATCCAAC1347110.7288922046176934No Hit
AACCGCTTCAAGTCTCAGCCCGAG1047420.5667364008586265No Hit
GCTTCAAGTCTCAGCCCGAG1036970.5610821309487789No Hit
CGCTTCAAGTCTCAGCCCGAGA1025860.5550707492551513No Hit
GCCTCGACCACGGCCAGTCCG1018720.5512074490488057No Hit
AGAATTCTACCACTGAACCACCAGTGC1002510.5424365672077884No Hit
GAGAATTCTACCACTGAACCACCAGTGC891310.4822686424254859No Hit
CTCCACTTCAGAGCAAAGATTTTCA847010.45829886663541397No Hit
CGCCCCAACGACCGGCAGCAGTCG807480.43691003510084186No Hit
CCGCTTCAAGTCTCAGCCCGAG763750.41324867403312526No Hit
CCACAAGTTCGGATCTACGGGT725800.3927147464657837No Hit
CGAGAATTCTACCACTGAACCACCAGTGC647900.35056473441055563No Hit
AAACTAACCAGCCTAGACTTGA584460.31623871689086797No Hit
CGTTTCAAGTCTCAGCCCGAG565920.30620712223741575No Hit
CTCCACCTCAGAGCAAAGATTTTCA549880.2975282237346447No Hit
TGCCAGTCCGGGCCGGATCCAAC534070.28897377327773643No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCG517630.28007844339085647No Hit
TCCACAGAATTATTTAAAAGTGATGCAAGA501800.27151317136474273No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC501630.2714211880264964No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC497610.26924605261620094No Hit
ACAAGTTCGGATCTACGGGT456510.24700772789900102No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAG433300.23444929683607615No Hit
GACCGCGACCTCGGAAAG401740.21737286062987593No Hit
GGCCAATCCACGGCCGATTCCTTCC388770.21035507300014153No Hit
CGGTCTCGGCGAGGAAGAACAGCTCGG376660.20380261284624152No Hit
CCAGAATTGCCTGAAGCCGTGCAGGAA356950.19313795639427045No Hit
GTAGACACTCAGACAGACATGCTCCTGGGAGAACC350270.18952355227965012No Hit
ACCCGAGGGTCACAGCTG347540.1880464080831062No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCG308430.16688482950184855No Hit
TGCTTCAAGTCTCAGCCCGAGA307550.16640868045680873No Hit
GCCAGAATTGCCTGAAGCCGTGCAGGAA305490.16529405882864737No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGC302750.1638115038475007No Hit
TGCCCCAACGACCGGCAGCAGTCG299750.1621882684666832No Hit
CAACCGCTTCAAGTCTCAGCCCGAG299510.1620584096362178No Hit
AGAATTCTACCACTGAACCACAGTGC299200.16189067531353332No Hit
AGCCAGTCCGGGCCGGATCCAAC290530.15719952506297072No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC283150.1532063660261596No Hit
CCACTTCAGAGCAAAGATTTTCA274840.1487100040212951No Hit
GGTCTCGGCGAGGAAGAACAGCTCGG273000.1477144196543937No Hit
CGGAAACGTTCGAGTTGGCTATCGGTAGTG272220.14729237845538115No Hit
CCACCTCAGAGCAAAGATTTTCA272110.14723285982475118No Hit
GAGAATTCTACCACTGAACCACAGTGC271770.14704889314825853No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG263800.14273649781988668No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC242270.13108707857021965No Hit
GGCCAATCCACGGCCGATTCCTTCCCGG240970.1303836765718654No Hit
CGGTCCGACCGCGACCTCGGAAAGGG236270.1278406078085846No Hit
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAG230570.12475646058503136No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGG230310.12461578018536049No Hit
AGCTGCTCCCACTTATTCTACACCTC229110.1239664860330335No Hit
GCCAGTCCGGGCCGGATCCAAC222850.12057933487172762No Hit
ATCCACTTCAGAGCAAAGATTTTCA220280.11918876322882727No Hit
GCCAGTCCGGGCCGGATCC218420.11818235729272042No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAG214130.11586113069815138No Hit
ATCCAACCGCTTCAAGTCTCAGCCCGAG212200.11481684926982544No Hit
GACCGCGACCTCGGAAAGGG210170.11371845999547225No Hit
GACCGCTTCAAGTCTCAGCCCGAG209930.11358860116500685No Hit
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAG209680.11345333154993874No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATA206920.11195995499958661No Hit
GGCCAGTCCGGGCCGGATCC201720.1091463470061696No Hit
TCCACTTCAGAGCAAAGATTTTCA200590.10853492834606167No Hit
TCCACCTCAGAGCAAAGATTTTCA198070.10717141062617495No Hit
CGAGAATTCTACCACTGAACCACAGTGC196070.10608925370562995No Hit
GGAATTCTACCACTGAACCACCAGTGC195000.10551029975313837No Hit
GACCGCGACCTCGGAAAGGGA192410.10410890654103258No Hit
CCTTCCTTAGGGAGTTACACAA191830.10379508103407452No Hit
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCG187500.10145221130109458No Hit
ACCTCGACCACGGCCAGTCCG187350.10137104953205371No Hit
GCCTCGACCACGGCCAGTCC185290.10025642790389236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)