FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POC-53-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-53-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18481608
Sequences flagged as poor quality0
Sequence length18-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG16677449.023803556487076No Hit
TGAAAATCTTTGCTCTGAAGTGGAA9531545.157310987225787No Hit
TGAAAATCTTTGCTCTGAGGTGGAA5595633.027674864654634No Hit
CTCGGGCTGAGACTTGAAGCA4929172.6670677140214205No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCG4200332.2727080890364086No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATTCCT2349921.2714910953635636TruSeq Adapter, Index 27 (100% over 35bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1708460.9244109062371629No Hit
GTTGGATCCGGCCCGGACTGGCC1377880.7455411888402784No Hit
CTCGGGCTGAGACTTGAAGC1077580.5830553272204453No Hit
CTCGGGCTGAGACTTGAAGCGGTT1036850.5610172015335462No Hit
CGGACTGGCCGTGGTCGAGGC1035900.5605031769962874No Hit
TCTCGGGCTGAGACTTGAAGCG1031650.5582035935401292No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1009960.5464676017368186No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC905780.4900980477456291No Hit
CGACTGCTGCCGGTCGTTGGGGCG780980.42257145590362044No Hit
CTCGGGCTGAGACTTGAAGCGG767590.4153264153205717No Hit
TGAAAATCTTTGCTCTGAAGTGGAG741890.4014206988915683No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG651800.3526749404056184No Hit
CGGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTC621980.33653998072029234No Hit
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATC601200.32529637031582964No Hit
TCAAGTCTAGGCTGGTTAGTTT580860.31429083443388695No Hit
CTCGGGCTGAGACTTGAAACG567450.3070349722816326No Hit
ACCCGTAGATCCGAACTTGTGG559250.3025981289073981No Hit
GTTGGATCCGGCCCGGACTGGCA532900.2883407114792176No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA526160.28469384265698094No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC512740.2774325697201239No Hit
TGAAAATCTTTGCTCTGAGGTGGAG503480.27242218317800054No Hit
TCTTGCATCACTTTTAAATAATTCTGTGGA502560.27192439099454985No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA495350.26802321529598505No Hit
ACCCGTAGATCCGAACTTGT447280.24201357371068577No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA434690.23520139589585493No Hit
CTTTCCGAGGTCGCGGTC410920.22233996089517755No Hit
GGAAGGAATCGGCCGTGGATTGGCC400060.21646384881661812No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCT393490.21290896333262777No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCG382590.20701120811565749No Hit
CAGCTGTGACCCTCGGGT355340.19226682007323173No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC318950.17257697490391527No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC311790.16870285312836417No Hit
TCTCGGGCTGAGACTTGAAGCA307580.16642491281061692No Hit
GCACTGTGGTTCAGTGGTAGAATTCT300850.16278345477298295No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA300130.16239387828158675No Hit
GTTGGATCCGGCCCGGACTGGCT291340.15763779861579144No Hit
CGACTGCTGCCGGTCGTTGGGGCA285470.15446166805399184No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT283270.1532712954413923No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC281040.15206469047498464No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG278810.15085808550857696No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC275370.14899677560523955No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG268230.14513347539889387No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC258310.13976597707299063No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT243990.13201773352188836No Hit
CCCTTTCCGAGGTCGCGGTCGGACCG240740.13025922852600272No Hit
TGAAAATCTTTGCTCTGAAGTGG239100.12937185985115582No Hit
TGAAAATCTTTGCTCTGAGGTGG233150.12615244301253442No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT231250.12512439393801666No Hit
GTTGGATCCGGCCCGGACTGGC230010.12445345664727873No Hit
GGATCCGGCCCGGACTGGC223030.12067672899457667No Hit
CCCTTTCCGAGGTCGCGGTC214400.11600722188242496No Hit
TGAAAATCTTTGCTCTGAAGTGGAT213730.11564469931404237No Hit
CTCGGGCTGAGACTTGAAGCGGTC212800.11514149634598894No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA212270.11485472476204452No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT211850.11462747180873006No Hit
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCT211600.11449220219366195No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA207840.11245774718303733No Hit
TGAAAATCTTTGCTCTGAAGTGGA207530.11229001286035284No Hit
GGATCCGGCCCGGACTGGCC205940.11142969810851956No Hit
TGAAAATCTTTGCTCTGAGGTGGA203140.10991467841975655No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC198630.10747441456392755No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCG197230.10671690471954604No Hit
TCCCTTTCCGAGGTCGCGGTC196700.10643013313560161No Hit
GGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTCT193050.104455196755607No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCT192320.10406020947960806No Hit
ACCCGTAGATCCGAACTTGGGG191770.10376261632645818No Hit
GGACTGGCCGTGGTCGAGGC187740.10158207013155997No Hit
CAGCTGTGACCCTCGGGTC186770.10105722402509565No Hit
CGGACTGGCCGTGGTCGAGGT186190.1007433985181376No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)