FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POC-50-S1-TP2.flexbar_trim.20230621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-50-S1-TP2.flexbar_trim.20230621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14858812
Sequences flagged as poor quality0
Sequence length18-35
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA312128321.006275602652487No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACC6677194.493757643612423No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT5471673.682441099598003Illumina RNA PCR Primer (100% over 35bp)
GGCGAGAATTCTACCACTGAACCACCAGTGC3376022.2720658959814553No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC1257230.8461174419596937No Hit
GAGAATTCTACCACTGAACCACCAGTGC1243080.8365944733670497No Hit
CGCTTCAAGTCTCAGCCCGAG1195220.8043846304805525No Hit
AAACTAACCAGCCTAGACTTGA994840.6695286271876918No Hit
TGCTTCAAGTCTCAGCCCGAG878200.5910297539264916No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC859290.5783032990793611No Hit
ACAAGTTCGGATCTACGGGT751430.5057133773547979No Hit
TCCACTTCAGAGCAAAGATTTTCA654490.44047262997876274No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC641590.4317909130285786No Hit
ATCCACTTCAGAGCAAAGATTTTCA638400.4296440388370214No Hit
GTAGACACTCAGACAGACATGCTCCTGGGAGAACC593140.39918399936684035No Hit
CCACAAGTTCGGATCTACGGGT568190.3823926165833446No Hit
GCCTCGACCACGGCCAGTCCG533560.3590865810806409No Hit
GCCAGAATTGCCTGAAGCCGTGCAGGAA484890.3263316071298298No Hit
AACCGCTTCAAGTCTCAGCCCGAG447260.30100656768522277No Hit
CTCCACTTCAGAGCAAAGATTTTCA445150.2995865349127508No Hit
AGAATTCTACCACTGAACCACCAGTGC421590.2837306239556702No Hit
GGCCAGTCCGGGCCGGATCCAAC379660.2555116788610018No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAG375680.25283313363141013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG373170.251143900333351No Hit
CCACTTCAGAGCAAAGATTTTCA370270.24919219652284447No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC347760.2340429369454301No Hit
GAGAATTCTACCACTGAACCACAGTGC340780.2293453877739351No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC333610.2245199683527862No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGAT309530.20831409671244241No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATA307680.20706904428160205No Hit
CCAGAATTGCCTGAAGCCGTGCAGGAA296240.19936990924981085No Hit
TCCCACTTCAGAGCAAAGATTTTCA275540.18543878205067807No Hit
TTCCACTTCAGAGTAAAGATTTTCA223760.1505907740134272No Hit
TCACAAGTTCGGATCTACGGGT218840.14727960754870578No Hit
TGCCAGTCCGGGCCGGATCCAAC216350.14560383427692605No Hit
TTCCACTTCAGAGCAAAGATTTTCG205580.13835561012549322No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGC192650.12965370313589No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGC186610.1255887751995247No Hit
GAACTAACCAGCCTAGACTTGA176900.1190539324408977No Hit
GCAAGTTCGGATCTACGGGT176370.11869724174449478No Hit
TGTCTTTCACCTACAGCATTAATA168930.11369011196857462No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC168060.11310460082542265No Hit
TCACTTCAGAGCAAAGATTTTCA167750.11289597041809264No Hit
AATGAGGTCAAAGTAGCGGATGAGGGGTG161150.1084541617459054No Hit
ACTCATCCTAAGTAGACTTTTCACATTGAA160300.10788211062903279No Hit
GTCGTCAGCGTTCAAATATGGAAACAGA158370.106583218093075No Hit
ACAGACCGAAAAACACAGGATGAATCTCAGTGGAT156380.10524394547827916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)