FastQCFastQC Report
Wed 21 Jun 2023
sRNA-POC-50-S1-TP2.flexbar_trim.20230621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-50-S1-TP2.flexbar_trim.20230621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14858812
Sequences flagged as poor quality0
Sequence length18-35
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA313611921.106122077592744No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCG6372694.288828743509239No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGAGC5365693.611116420343699TruSeq Adapter, Index 1 (96% over 33bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3455612.3256300705601496No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1263020.8500141195675671No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1253270.8434523567563813No Hit
CTCGGGCTGAGACTTGAAGCG1210600.8147353906893768No Hit
TCAAGTCTAGGCTGGTTAGTTT998670.6721062222201882No Hit
CAACTTTTGACGGTGGATCTCTAGGCTCACGCATC970260.652986254890364No Hit
CTCGGGCTGAGACTTGAAGCA879460.5918777355820909No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC879130.5916556451484816No Hit
ACCCGTAGATCCGAACTTGT761400.5124232004550566No Hit
TGAAAATCTTTGCTCTGAAGTGGA679060.4570082722629508No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT644180.4335339864317551No Hit
TGAAAATCTTTGCTCTGAAGTGGAT623060.4193201986807559No Hit
ACCCGTAGATCCGAACTTGTGG591130.39783126672576513No Hit
CGGACTGGCCGTGGTCGAGGC543370.36568872397066465No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC497690.33494602394861717No Hit
CTCGGGCTGAGACTTGAAGCGGTT449500.30251409062851053No Hit
TGAAAATCTTTGCTCTGAAGTGGAG447730.3013228783027876No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT426410.28697449028899485No Hit
GTTGGATCCGGCCCGGACTGGCC385770.2596237168893449No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA377300.25392339576003786No Hit
TGAAAATCTTTGCTCTGAAGTGG373190.2511573603596304No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA346340.23308727507959587No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC345640.23261617415981842No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT336880.2267206826494608No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA309060.20799778609487757No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG298540.20091781227193667No Hit
TGAAAATCTTTGCTCTGAAGTGGGA277730.18691265492826747No Hit
ACCCGTAGATCCGAACTTGTGA226920.1527174581655653No Hit
TGAAAATCTTTACTCTGAAGTGGAA225080.15147913574786465No Hit
GTTGGATCCGGCCCGGACTGGCA216290.14556345419808797No Hit
CGAAAATCTTTGCTCTGAAGTGGAA204420.13757492860129059No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTCG202030.13596645546090763No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCA194770.13108046592150166No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTA186410.12545417493673117No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA183760.12367072145471657No Hit
ACCCGTAGATCCGAACTTGC181420.12209589838003199No Hit
TCAAGTCTAGGCTGGTTAGTTC179420.12074989575209646No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCT175200.11790983020715248No Hit
TATTAATGCTGTAGGTGAAAGACA169770.11425543307230752No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT168170.11317863096995912No Hit
TGAAAATCTTTGCTCTGAAGTGA168080.11311806085170201No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAAAAA166280.11190665848656003No Hit
CACCCCTCATCCGCTACTTTGACCTCATT164930.11099810671270353No Hit
TCTGTTTCCATATTTGAACGCTGACGAC162360.10926849333580638No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT161100.108420511680207No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA149410.10055312631992383No Hit
CTTTCCGAGGTCGCGGTC148850.10017624558410188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)